<i>Staphylococcus lugdunensis</i>Infection Sites: Predominance of Abscesses in the Pelvic Girdle Region

Bellamy, Richard; Barkham, Timothy

doi:10.1086/341304

Cited by 54 publications

(45 citation statements)

References 10 publications

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“…It is a commensal of human skin and can occasionally cause serious invasive infections such as osteomyelitis, peritonitis, soft tissue abscesses and infective endocarditis, diseases that are more usually associated with Staphylococcus aureus (Van der Mee-Marquet et al, 2003;Bellamy & Barkham 2002;Kragsbjerg et al, 2000). S. lugdunensis accounts for 18 % of infective endocarditis and 44 % of native valve endocarditis caused by CoNS (Patel et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the fibrinogen-binding surface protein Fbl of Staphylococcus lugdunensis

2004

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The fbl gene of Staphylococcus lugdunensis encodes a protein Fbl that is 58 % identical to the clumping factor A (ClfA) of Staphylococcus aureus. The fbl gene was present in eight clinical isolates of S. lugdunensis. When Fbl was expressed on the surface of Lactococcus lactis it promoted adherence to immobilized fibrinogen and cell clumping in a fibrinogen solution. Purified recombinant Fbl region A bound to immobilized fibrinogen in a dose-dependent manner and inhibited the adherence of both Fbl-expressing and ClfA-expressing strains of L. lactis to fibrinogen. Adherence of S. lugdunensis and L. lactis Fbl + to immobilized fibrinogen was also inhibited by rabbit anti-Fbl region A antibodies and rabbit anti-ClfA region A antibodies, as well as by human immunoglobulin with a high level of anti-ClfA antibodies. Alignment of the A domains of CflA and Fbl revealed that all of the ClfA residues implicated in binding to the c-chain of fibrinogen are conserved in Fbl. Nevertheless Fbl had a tenfold lower affinity for fibrinogen, suggesting that sequence differences that occur elsewhere in the protein, possibly in b-strand E of domain N2, affect ligand binding.

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Section: Introductionmentioning

confidence: 99%

Characterization of the fibrinogen-binding surface protein Fbl of Staphylococcus lugdunensis

2004

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“…Other infections such as osteomyelitis (17,23), peritonitis (28), intravascular catheter infections (5,32), prosthetic joint infections (26), and urinary tract infections have also been reported (10). S. lugdunensis has been reported as an important cause also of skin and soft tissue infections (SSTI), primarily in the groin (1,39) and mammae (13,22,25,37,41).…”

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confidence: 99%

“…lugdunensis is part of the normal human skin flora and commonly colonizes the perineal region (1,37,39); however, carriage rates of different CoNS are not comprehensively accounted for, and only a few have looked for S. lugdunensis carriage (13,16,39).…”

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confidence: 99%

Staphylococcus lugdunensis , a Common Cause of Skin and Soft Tissue Infections in the Community

et al. 2009

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Staphylococcus lugdunensis, a rare cause of severe infections such as native valve endocarditis, often causes superficial skin infections similar to Staphylococcus aureus infections. We initiated a study to optimize the identification methods in the routine laboratory, followed by a population-based epidemiologic analysis of patients infected with S. lugdunensis in Viborg County, Denmark. Recognition of a characteristic Eikenella corrodens-like odor on Columbia sheep blood agar combined with colony pleomorphism and prominent ␤-hemolysis after 2 days of incubation, confirmed by API-ID-32 Staph, led to an 11-fold increase in the detection of S. lugdunensis. By these methods we found 491 S. lugdunensis infections in 4 years, corresponding to an incidence of 53 per 100,000 per year, an increase from 5 infections per 100,000 inhabitants in the preceding years. Seventy-five percent of the cases were found in general practice; these were dominated by skin abscesses (36%), wound infections (25%), and paronychias (13%). Fifty-six percent of the infections occurred below the waist, and toes were the most frequently infected site (21%). Only 3% of the patients suffered from severe invasive infections. The median age was 52 years, and the male/female ratio was 0.69. Our study shows that S. lugdunensis is a common cause of skin and soft-tissue infections (SSTI) and is probably underrated by many laboratories. S. lugdunensis should be accepted as a significant pathogen in SSTI and should be looked for in all routine bacteriological examinations, and clinicians should be acquainted with the name and the pathology of the bacterium.Staphylococcus lugdunensis, one of the coagulase-negative staphylococci (CoNS), was first described by Freney et al. 20 years ago (8). It causes severe infections similar to Staphylococcus aureus infections, especially acute endocarditis in prosthetic and native valves (14,24,29,30,36). Other infections such as osteomyelitis (17, 23), peritonitis (28), intravascular catheter infections (5, 32), prosthetic joint infections (26), and urinary tract infections have also been reported (10). S. lugdunensis has been reported as an important cause also of skin and soft tissue infections (SSTI), primarily in the groin (1, 39) and mammae (13,22,25,37,41).S. lugdunensis is part of the normal human skin flora and commonly colonizes the perineal region (1, 37, 39); however, carriage rates of different CoNS are not comprehensively accounted for, and only a few have looked for S. lugdunensis carriage (13, 16, 39).Our study was undertaken after two serious cases of S. lugdunensis infection, a fatal case of endocarditis with protracted bacterial identification but correct antibiotic treatment and a case of late-diagnosed osteomyelitis, which prompted an optimization of our routine microbiological methods for identification of S. lugdunensis. This subsequently increased the number of S. lugdunensis isolates significantly, especially in samples from patients in general practice, and encouraged us to conduct a prospectiv...

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“…S. xylosus have been identified in high numbers in dried salted cod (Vilhelmsson et al, 1997;Doe and Heruwati, 1988) and are rarely associated with human or animal infections (Kloos and Schleifer, 1986). S. lugdunensis is a common human skin commensal (Bellamy and Barkham, 2002;Vandenesch et al, 1995;Van der Mee-Marquet, 2003). These bacteria display pathogen characteristics, although they do not belong to this group, and exhibit pathogenicity similar to Staphylococcus aureus, with high associated morbidity and mortality (Cercenado, 2009;Frank and Patel, 2008;Poutanen and Baron, 2001).…”

Section: Bacterial Strain Identificationmentioning

confidence: 99%

Microbiota of freshwater catfish species, Filhote (Brachyplatystoma filamentosum) and Dourada (Brachyplatystoma rousseauxii) from the Amazon Region (Belm-Par-Brazil)

Evelyn¹,

Lauana²,

Hamilton³

et al. 2016

Afr. J. Microbiol. Res.

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This paper identified the bacterial flora composition and its influence on the microbiological spoilage process in economically important fish species: Filhote (Brachyplatystoma filamentosum) and Dourada (Brachyplatystoma rousseauxii) in the Amazon region. Microbiological characterization was performed: counts of total mesophilic aerobic bacteria, psychrotrophic bacteria and coliforms at 35 and 45°C, respectively. Bacteria were also isolated through seeding in agar surface using Violet Red Bile Glucose (VRBG) for enterobacteria strains and Baird-Parker Agar with Egg-Yolk Tellurite for Staphylococcus species, both with incubation at 36°C for 48 h. The bacteria isolated were identified using the API 20E kit (Enterobacteria), and Gram-positive bacteria with API Staph (Staphylococci). Finally, the lag phase for strain growth was measured using spectrophotometry readings (620 nm) at different temperatures (10, 15 and 37°C). The mesophilic aerobic bacteria counts for fresh fish samples ranged from 5.21 to 7.64. The count ranges of psychrotrophic aerobic bacteria found were 5.11 -6.91 log CFU/g. They also had an average score above 10 3 MPN/g for total coliforms. The most predominant were Hafnia alvei, Pseudomonas luteola, Staphylococcus xylosus and Staphylococcus lugdunensis. When subjected to temperature of 10°C, the strains achieved growth after 6 h (p<0.05).

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Staphylococcus lugdunensisInfection Sites: Predominance of Abscesses in the Pelvic Girdle Region

Cited by 54 publications

References 10 publications

Characterization of the fibrinogen-binding surface protein Fbl of Staphylococcus lugdunensis

Characterization of the fibrinogen-binding surface protein Fbl of Staphylococcus lugdunensis

Staphylococcus lugdunensis , a Common Cause of Skin and Soft Tissue Infections in the Community

Microbiota of freshwater catfish species, Filhote (Brachyplatystoma filamentosum) and Dourada (Brachyplatystoma rousseauxii) from the Amazon Region (Belm-Par-Brazil)

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