<i>Staphylococcus haemolyticus</i>
 as a potential producer of biosurfactants with antimicrobial, anti-adhesive and synergistic properties

Rossi, Ciro César; Santos-Gandelman, Juliana F.; Barros, Elaine M.; Alvarez, Vanessa Marques; Laport, Marinella Silva; Giambiagi-deMarval, Márcia

doi:10.1111/lam.12611

Cited by 20 publications

(19 citation statements)

References 21 publications

(44 reference statements)

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“…Because S. haemolyticus is one of the most important CoNS and no CRISPRs have been described to the species so far, the multiplex PCR reaction was employed in 59 clinical strains of this species previously shown by our group to be phenotypic and genetically diverse ( Rossi et al, 2016b ). The cas1 gene was amplified in only 3% of the strains (2/59), namely MD03 and MD25.…”

Section: Resultsmentioning

confidence: 99%

“…Both cas1IIF/R and cas1IIIF/R primer pairs were used in a multiplex polymerase chain reaction (PCR) reaction containing 2 U of the GoTaq G2 DNA Polymerase (Promega, United States) in the enzyme buffer, containing 0.2 mM of each dNTP, 0.2 mM of each primer and 0.1 mM of the primer pair 16S rRNAF/R ( Chen et al, 2013 ) as an endogenous control. Screening was performed in 59 clinical strains of Staphylococcus haemolyticus previously analyzed by our group and shown to be phenotypic and genetically diverse ( Barros et al, 2012 ; Rossi et al, 2016b ). Amplification was performed in a T100 Thermal Cycler (BioRad, United States) with the following cycle parameters: initial denaturation at 95°C for 3 min, followed by 30 cycles (95°C for 30 s, 50°C for 1 min, and 72°C for 1 min) and 72°C for 3 min.…”

Section: Methodsmentioning

confidence: 99%

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CRISPR-Cas Systems Features and the Gene-Reservoir Role of Coagulase-Negative Staphylococci

et al. 2017

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The claimed role of gene reservoir of coagulase-negative staphylococci (CoNS) could be contradicted by estimates that CRISPR/Cas systems are found in the genomes of 40–50% of bacteria, as these systems interfere with plasmid uptake in staphylococci. To further correlate this role with presence of CRISPR, we analyzed, by computational methods, 122 genomes from 15 species of CoNS. Only 15% of them harbored CRISPR/Cas systems, and this proportion was much lower for S. epidermidis and S. haemolyticus, the CoNS most frequently associated with opportunistic infections in humans. These systems are of type II or III, and at least two of them are located within SCCmec, a mobile genetic element of Staphylococcus bacterial species. An analysis of the spacers of these CRISPRs, which come from exogenous origin, allowed us to track the transference of the SCCmec, which was exchanged between different strains, species and hosts. Some of the spacers are derived from plasmids described in Staphylococcus species that are different from those in which the CRISPR are found, evidencing the attempt (and failure) of plasmid transference between them. Based on the polymorphisms of the cas1 gene in CRISPRs of types II and III, we developed a multiplex polymerase chain reaction (PCR) suitable to screen and type CRISPR systems in CoNS. The PCR was tested in 59 S. haemolyticus strains, of which only two contained a type III cas1. This gene was shown to be expressed in the exponential growth, stationary phase and during biofilm formation. The low abundance of CRISPRs in CoNS is in accordance with their role as gene reservoirs, but when present, their spacers sequence evidence and give an insight on the dynamics of horizontal genetic transfer among staphylococci.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

CRISPR-Cas Systems Features and the Gene-Reservoir Role of Coagulase-Negative Staphylococci

et al. 2017

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“…Rossi et al . () showed that some strains of biosurfactant‐producing Staphylococcus haemolyticus had antimicrobial activity against a range of Gram‐positive and Gram‐negative bacteria and subinhibitory concentrations of the biosurfactant were able to decrease biofilm formation and showed synergistic effects with tetracycline.…”

Section: Antimicrobial and Antifungal Properties Of Biosurfactantsmentioning

confidence: 99%

“…Ghribi and Ellouze-Chaabouni (2011) isolated a biosurfactant-producing strain B. subtilis SPB1 (HQ392822) and identified antimicrobial activity against micro-organisms with multidrug-resistant profiles (Ghribi et al 2012). Rossi et al (2016) showed that some strains of biosurfactant-producing Staphylococcus haemolyticus had antimicrobial activity against a range of Gram-positive and Gram-negative bacteria and subinhibitory concentrations of the biosurfactant were able to decrease biofilm formation and showed synergistic effects with tetracycline.…”

Section: Antimicrobial and Antifungal Properties Of Biosurfactantsmentioning

confidence: 99%

Microbial biosurfactants: current trends and applications in agricultural and biomedical industries

et al. 2019

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Synthetic surfactants are becoming increasingly unpopular in many applications due to previously disregarded effects on biological systems and this has led to a new focus on replacing such products with biosurfactants that are biodegradable and produced from renewal resources. Microbially derived biosurfactants have been investigated in numerous studies in areas including: increasing feed digestibility in an agricultural context, improving seed protection and fertility, plant pathogen control, antimicrobial activity, antibiofilm activity, wound healing and dermatological care, improved oral cavity care, drug delivery systems and anticancer treatments. The development of the potential of biosurfactants has been hindered somewhat by the myriad of approaches taken in their investigations, the focus on pathogens as source species and the costs associated with large‐scale production. Here, we focus on various microbial sources of biosurfactants and the current trends in terms of agricultural and biomedical applications.

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“…38 Soil contaminations with hydrocarbons are widely proven to be persistent in nature, poorly soluble in water, and often associated with toxic and carcinogenic effects. 39 Current soil remediation techniques require basically heavy, expensive equipments, and even display several environmental risks. 40 The low water solubility of hydrocarbons constitutes one of the main problems affecting their biodegradation efficiency.…”

Section: Discussionmentioning

confidence: 99%

Promising abilities of mercapto‐degrading Staphylococcus capitis strain SH6 in both crude oil and waste motor oil as sole carbon and energy sources: its biosurfactant production and preliminary characterization

Chebbi

Hentati

Cheffi

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND It was shown previously that a Staphylococcus capitis strain SH6 was able to degrade several malodorous mercaptans and simultaneously reduce the surface tension. RESULTS This present work revealed the capacity of strain SH6 to grow on various hydrocarbons, used as the only carbon and energy sources. Based on GC–MS analyses, the substantial ability to degrade up to 45% and 64% of aliphatic hydrocarbons (n‐alkanes) of crude oil and waste motor oil, respectively, after 30 days of incubation at 37 °C and 180 rpm, was shown. The properties of biosurfactant produced by strain SH6 grown on different oil substrates (diesel oil and waste motor oil) were studied. Biosurfactants exhibited enhanced emulsification capacities and significant stabilities over a wide range of salinity (20–150 g L‐1), temperature (–20–100 °C), and pH , and also the ability to remove crude oil from contaminated soils. Their critical micelle concentrations (CMC) were of 800 mg L‐1. FTIR analyses suggested the lipopetide nature of biosurfactants. CONCLUSION The stabilities of biosurfactants over a wide pH range, high temperatures and variable concentrations of salt, as well as emulsifying properties, suggest potential applications in bioremediation processes. © 2017 Society of Chemical Industry

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Staphylococcus haemolyticus as a potential producer of biosurfactants with antimicrobial, anti-adhesive and synergistic properties

Cited by 20 publications

References 21 publications

CRISPR-Cas Systems Features and the Gene-Reservoir Role of Coagulase-Negative Staphylococci

CRISPR-Cas Systems Features and the Gene-Reservoir Role of Coagulase-Negative Staphylococci

Microbial biosurfactants: current trends and applications in agricultural and biomedical industries

Promising abilities of mercapto‐degrading Staphylococcus capitis strain SH6 in both crude oil and waste motor oil as sole carbon and energy sources: its biosurfactant production and preliminary characterization

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