<i>Staphylococcus epidermidis</i>
 biofilm on implant material is reduced by a covalently linked thiophenone

Scheie, Anne Aamdal; Chamgordani, Elahe Jafari; Naemi, Ali-Oddin; Hansen, Finn Knut; Benneche, T.

doi:10.1111/jam.13188

Cited by 6 publications

(4 citation statements)

References 21 publications

(36 reference statements)

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“…A wide range of different implant materials are used in orthopedics. Despite the intensive research currently being performed on different technologies for incorporating antimicrobial agents [22][23][24][25][26][27][28][29][30][31][32], only a few studies investigating biofilm formation on different clinically relevant implantation materials have been published [33][34][35][36][37][38]. The dynamic changes associated with biofilm growth [39] make biofilm eradication from clinical materials even more challenging.…”

Section: Introductionmentioning

confidence: 99%

Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

et al. 2019

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Medical device-associated staphylococcal infections are a common and challenging problem. However, detailed knowledge of staphylococcal biofilm dynamics on clinically relevant surfaces is still limited. In the present study, biofilm formation of the Staphylococcus aureus ATCC 25923 strain was studied on clinically relevant materials—borosilicate glass, plexiglass, hydroxyapatite, titanium and polystyrene—at 18, 42 and 66 h. Materials with the highest surface roughness and porosity (hydroxyapatite and plexiglass) did not promote biofilm formation as efficiently as some other selected materials. Matrix-associated poly-N-acetyl-β-(1-6)-glucosamine (PNAG) was considered important in young (18 h) biofilms, whereas proteins appeared to play a more important role at later stages of biofilm development. A total of 460 proteins were identified from biofilm matrices formed on the indicated materials and time points—from which, 66 proteins were proposed to form the core surfaceome. At 18 h, the appearance of several r-proteins and glycolytic adhesive moonlighters, possibly via an autolysin (AtlA)-mediated release, was demonstrated in all materials, whereas classical surface adhesins, resistance- and virulence-associated proteins displayed greater variation in their abundances depending on the used material. Hydroxyapatite-associated biofilms were more susceptible to antibiotics than biofilms formed on titanium, but no clear correlation between the tolerance and biofilm age was observed. Thus, other factors, possibly the adhesive moonlighters, could have contributed to the observed chemotolerant phenotype. In addition, a protein-dependent matrix network was observed to be already well-established at the 18 h time point. To the best of our knowledge, this is among the first studies shedding light into matrix-associated surfaceomes of S. aureus biofilms grown on different clinically relevant materials and at different time points.

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Section: Introductionmentioning

confidence: 99%

Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

et al. 2019

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“…These specific antibiofilm compounds are believed to be less prone to resistance development. They could be used in several applications, one of which is as antibiofilm coatings on the surface of implantable medical devices, such as orthopedic implants or dental implants ( 22 – 25 ).…”

Section: Introductionmentioning

confidence: 99%

Modulation of the Substitution Pattern of 5-Aryl-2-Aminoimidazoles Allows Fine-Tuning of Their Antibiofilm Activity Spectrum and Toxicity

Peeters

Hooyberghs

Robijns

et al. 2016

Antimicrob Agents Chemother

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We previously synthesized several series of compounds, based on the 5-aryl-2-aminoimidazole scaffold, that showed activity preventing the formation of Salmonella enterica serovar Typhimurium and Pseudomonas aeruginosa biofilms. Here, we further studied the activity spectrum of a number of the most active N1- and 2N-substituted 5-aryl-2-aminoimidazoles against a broad panel of biofilms formed by monospecies and mixed species of bacteria and fungi. An N1-substituted compound showed very strong activity against the biofilms formed by Gram-negative and Gram-positive bacteria and the fungus Candida albicans but was previously shown to be toxic against various eukaryotic cell lines. In contrast, 2N-substituted compounds were nontoxic and active against biofilms formed by Gram-negative bacteria and C. albicans but had reduced activity against biofilms formed by Gram-positive bacteria. In an attempt to develop nontoxic compounds with potent activity against biofilms formed by Gram-positive bacteria for application in antibiofilm coatings for medical implants, we synthesized novel compounds with substituents at both the N1 and 2N positions and tested these compounds for antibiofilm activity and toxicity. Interestingly, most of these N1-,2N-disubstituted 5-aryl-2-aminoimidazoles showed very strong activity against biofilms formed by Gram-positive bacteria and C. albicans in various setups with biofilms formed by monospecies and mixed species but lost activity against biofilms formed by Gram-negative bacteria. In light of application of these compounds as anti-infective coatings on orthopedic implants, toxicity against two bone cell lines and the functionality of these cells were tested. The N1-,2N-disubstituted 5-aryl-2-aminoimidazoles in general did not affect the viability of bone cells and even induced calcium deposition. This indicates that modulating the substitution pattern on positions N1 and 2N of the 5-aryl-2-aminoimidazole scaffold allows fine-tuning of both the antibiofilm activity spectrum and toxicity.

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“…), уменьшающая содержание там микроорганизмов, является основным элементом для нехирургической профилактики периимплантитов. В связи с этим постоянно разрабатываются новые методы сниже ния степени адсорбции микроорганизмов для пред отвращения формирования микробных биофиль мов на поверхности изделий [23,[26][27][28][29][30][31][32][33][34].…”

Section: придание антибактериальных свойств имплантируемым материаламunclassified

“…Тиофенон соединяется с нержавеющей сталью ковалентной связью. Нанесение на поверхность ме таллических изделий соединений тиофенона, инги бирует формирование биофильма из S. epidermidis, который является частой причиной инфекций, свя занных с имплантированием инородных тел [26]. Добавление цинка в состав гидроксиапатита на по верхности титанового имплантата может способ ствовать антимикробному действию и улучшить остеоинтеграцию [31].…”

Section: модификация характера поверхности изделий для придания антибunclassified

The features of interaction between dental implants and organism tissues and the modern methods of creation of antibacterial covering on implant surfaces

Maiborodin¹,

Shevela²,

Toder³

et al. 2017

Ross. stomatol.

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Материалы, используемые для изготовления дентальных имплантатов, должны обладать механической прочностью, высокой биосовместимостью, отсутствием биодеградации и в основном должны решать две задачи: улучшение интеграции инородных тел с живыми тканями организма и борьбу с периимплантной инфекцией. Обе неурегулированные проблемы могут стать причиной отторжения искусственных изделий. Для выявления современного положения дел по выбору материалов для изготовления дентальных имплантатов и приданию им антибактериальных свойств была изучена научная литература за последние 2 года. Принимая во внимание большое число разноречивых результатов исследований, посвященных каждой из проблем дентальной имплантации, можно сделать заключение, что ни одна из основных задач окончательно не решена. Продолжаются поиски материалов, которые взаимодействуют с биологическими тканями без развития сопутствующего воспалительного процесса. Также не закончены разработки методов, направленных на профилактику и борьбу с периимплантной инфекцией. Формирование костной ткани на границе имплантата, срастание его поверхности с костью, несомненно, является благоприятным признаком, свидетельствующим о стабильности установки изделия, долгосрочности его службы. Необходима тщательная обработка поверхности имплантатов для исключения попадания в ткани даже мельчайших его частиц, что может поставить под сомнение успешность самой процедуры дентальной имплантации. Целесообразно изготавливать все детали имплантата из одного материала для профилактики электрохимической коррозии изделий в тканях на границе различных металлов. Перспективно нанесение покрытия на дентальные имплантаты, которое не только обладает антимикробным свойством, но и улучшает процессы их остеоинтеграции. Также представляется эффективной активная или пассивная адсорбция бактериофага или смеси нескольких бактериофагов на поверхности имплантируемых материалов. Необходимо отметить, что качество изделий, внедряемых в организм, постоянно улучшается, постепенно совершенствуются их различные характеристики, что способствует повышению эффективности ближайших и отдаленных результатов хирургического вмешательства-дентальной имплантации. Ключевые слова: обзор литературы, дентальная имплантация, имплантируемые материалы, взаимодействие искусственных материалов с тканями организма, антибактериальные свойства имплантатов. The features of interaction between dental implants and organism tissues and the modern methods of creation of antibacterial covering on implant surfaces

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Staphylococcus epidermidis biofilm on implant material is reduced by a covalently linked thiophenone

Abstract: Functionalizing surfaces by covalent linking of thiophenones might open a wide array of applications. Thiophenone coating of medical implants represents a novel and promising approach to prevent implant-associated infections.

Cited by 6 publications

References 21 publications

Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

Modulation of the Substitution Pattern of 5-Aryl-2-Aminoimidazoles Allows Fine-Tuning of Their Antibiofilm Activity Spectrum and Toxicity

The features of interaction between dental implants and organism tissues and the modern methods of creation of antibacterial covering on implant surfaces

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