<i>Sporothrix schenckii</i> — a freeze‐fracture study

Svoboda, Augustín; Trujillo‐Gonzalez, Amanda

doi:10.1002/jobm.3620300514

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…Grooves that are almost identical to those observed in the plasma membrane of S. cerevisiae have been found in several other yeast species, such as Candida albicans (Barug and de Groot, 1985) and Schizosaccharomyces pombe (Takeo, 1984), in other fungi, such as Lichinella stipatula (Büdel and Rhiel, 1987) and Spotothrix schenckii (Svoboda and Trujillo-Gonzalez, 1990), in bacteria, such as Micrococcus (Sleytr and Kocur, 1971;Sleytr et al, 1976) and in green algae, including several Chlamydomonas species (Clarke and Leeson, 1985). Similar structures were also observed in higher plants, such as Plumbago zeylanica (Southworth et al, 1997).…”

Section: Discussionsupporting

confidence: 59%

Furrow-like invaginations of the yeast plasma membrane correspond to membrane compartment of Can1

Strádalová

Stahlschmidt

Großmann

et al. 2009

Journal of Cell Science

152

187

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exhibiting elongated MCC patches, there are elongated invaginations of the appropriate size and frequency. Using various approaches of immunoelectron microscopy, the MCC protein Sur7, as well as the eisosome marker Pil1, have been detected at these invaginations. Thus, we identify the MCC patch, which is a lateral membrane domain of specific composition and function, with a specific structure in the yeast plasma membrane -the furrow-like invagination.

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Section: Discussionsupporting

confidence: 59%

Furrow-like invaginations of the yeast plasma membrane correspond to membrane compartment of Can1

Strádalová

Stahlschmidt

Großmann

et al. 2009

Journal of Cell Science

152

187

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“…In addition, the SsDRK1-i mutant displayed abnormalities in the ultrastructure of yeast cells, with variations observed in plasma membrane invaginations. Unlike wild-type yeast cells, invaginations in the SsDRK1-i yeast cells were short and abundant, being more similar to the conidia of the mycelia phase ( 22 ). This abnormality may account for the irregular morphology of spores in the SsDRK1-i strain, as detected by scanning electron microscopy.…”

Section: Discussionmentioning

confidence: 99%

Two‑component histidine kinase DRK1 is required for pathogenesis in Sporothrixï¿½schenckii

Zhang

Hou

et al. 2017

Mol Med Report

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Sporothrix schenckii is a pathogenic dimorphic fungus with a global distribution. It grows in a multicellular hyphal form at 25°C and a unicellular yeast form at 37°C. The morphological switch from mold to yeast form is obligatory for establishing pathogenicity in S. schenckii. Two-component signaling systems are utilized by eukaryotes to sense and respond to external environmental changes. DRK1is a hybrid histidine kinase, which functions as a global regulator of dimorphism and virulence in Blastomyces dermatitidis and Histoplasma capsulatum. An intracellular soluble hybrid histidine kinase, homologous to DRK1 in B. dermatitidis, has previously been identified in S. schenckii and designated as SsDRK1. In the present study, the function of SsDRK1 was investigated using double stranded RNA interference mediated by Agrobacterium tumefaciens. SsDRK1 was demonstrated to be required for normal asexual development, yeast-phase cell formation, cell wall composition and integrity, melanin synthesis, transcription of the morphogenesis-associated gene Ste20 that is involved in the high osmolarity glycerol/mitogen-activated protein kinase pathway, and pathogenicity of S. schenckii in a murine model of cutaneous infection. Further investigations into the signals SsDRK1 responds to, and the interactions of upstream transmembrane hybrid histidine kinases with SsDRK1, are required to uncover novel targets for anti-fungal therapies.

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“…There are characteristic differences in cell wall thickness between conidia, yeast forms, and filaments, as well variations in plasma membrane invaginations among these three morphological forms of the fungus. It has been shown by freeze fracturing studies that in conidia invaginations are short and abundant and in yeast forms they are scarce and longer, while the plasma membrane of the S. schenckii hyphae is smooth, without invaginations (244).…”

Section: Cell Wallmentioning

confidence: 99%

Sporothrix schenckii and Sporotrichosis

Barros

Paes²,

Schubach³

2011

Clin Microbiol Rev

523

677

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SUMMARY Sporotrichosis, which is caused by the dimorphic fungus Sporothrix schenckii , is currently distributed throughout the world, especially in tropical and subtropical zones. Infection generally occurs by traumatic inoculation of soil, plants, and organic matter contaminated with the fungus. Certain leisure and occupational activities, such as floriculture, agriculture, mining, and wood exploitation, are traditionally associated with the mycosis. Zoonotic transmission has been described in isolated cases or in small outbreaks. Since the end of the 1990s there has been an epidemic of sporotrichosis associated with transmission by cats in Rio de Janeiro, Brazil. More than 2,000 human cases and 3,000 animal cases have been reported. In humans, the lesions are usually restricted to the skin, subcutaneous cellular tissue, and adjacent lymphatic vessels. In cats, the disease can evolve with severe clinical manifestations and frequent systemic involvement. The gold standard for sporotrichosis diagnosis is culture. However, serological, histopathological, and molecular approaches have been recently adopted as auxiliary tools for the diagnosis of this mycotic infection. The first-choice treatment for both humans and cats is itraconazole.

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Sporothrix schenckii — a freeze‐fracture study

Cited by 4 publications

References 18 publications

Furrow-like invaginations of the yeast plasma membrane correspond to membrane compartment of Can1

Furrow-like invaginations of the yeast plasma membrane correspond to membrane compartment of Can1

Two‑component histidine kinase DRK1 is required for pathogenesis in Sporothrixï¿½schenckii

Sporothrix schenckii and Sporotrichosis

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