<i>Special feature: Tutorial</i>. Desorption ionization mass spectrometry

Busch, Kenneth L.

doi:10.1002/jms.1190300202

Cited by 72 publications

(47 citation statements)

References 20 publications

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“…Taking into account the pioneering works of several research groups in the early 1980s [1][2][3][4][5][6][7][8][9], which investigated direct laser desorption/ionization of organic materials, we first tried to analyze our peptide samples without any substrate by depositing the peptide solution directly on the stainless steel MALDI plate. The results were very promising in terms of sensitivity and detection mass range but the low mass part of the spectra was exhibiting a lot of signals ( Figure 3).…”

Section: Preliminary Ldi Analysesmentioning

confidence: 99%

“…The results gathered from synthetic peptide cocktails indicated that LDI mass spectrometry on silica gel or alumina constitutes a promising complementary method to MALDI in proteomics for peptide mass fingerprinting. [1][2][3][4][5][6][7][8][9] was investigated by Siuzdak and collaborators using porous silicon as the substrate [10]. This desorption/ionization on porous silicon (DIOS) mass spectrometry [10] allows the analysis of various compounds, such as small organic molecules [11], amino acids [12], peptides [13], and fatty acids [14].…”

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confidence: 99%

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Laser desorption/ionization mass spectrometry on porous silica and alumina for peptide mass fingerprinting

Shenar

Martinez

Enjalbal

2008

J. Am. Soc. Mass Spectrom.

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We investigated a variant of desorption/ionization on porous silicon (DIOS) mass spectrometry utilizing an aqueous suspension of either porous silica gel or porous alumina (pore size of 60 and 90 Å, respectively). Laser desorption/ionization (LDI) from samples directly deposited on a stainless steel surface without any inorganic substrates was also achieved. Synthetic peptides designed to cover large sequence diversity constituted our model compounds. Sample preparation, including material conditioning, peptide solubilization, and deposition protocol onto standard matrix-assisted laser desorption/ionization (MALDI) probe, as well as ionization source tuning were optimized to perform sensitive reproducible LDI analyses. The addition of either a cationizing agent or an alkali metal scavenger to the sample suspension allowed modification of the ionization output. Comparing hydrophilic silica gel to hydrophobic reversed-phase silica gel as well as increasing material pore size provided further insights into desorption/ionization processes. Furthermore, mixtures of peptides were analyzed to probe the spectral suppression phenomenon when no interfering organic matrix was present. The results gathered from synthetic peptide cocktails indicated that LDI mass spectrometry on silica gel or alumina constitutes a promising complementary method to MALDI in proteomics for peptide mass fingerprinting. [19]. These LDI methods, sometimes referred to as "matrix-free", produce mass spectra that exhibit less matrix ions in the low mass range compared with the chemical noise generated by the organic matrices used in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. Such chemical noise that usually pollutes the low mass range of MALDI mass spectra and that may complicate the detection of small molecules is less pronounced in the DIOS mass spectra.Nevertheless, the drawback of such an approach is in the preparation of the silicon material. To present adequate physical properties [20], silicon surfaces are conditioned via a galvanostatic etching procedure from Si wafer to produce pores with nanometer diameters. Commercially available ready-to-use DIOS-chips can also be mounted on modified MALDI plates [20]. Otherwise, silicon powders (5 to 50 nm particle size) were investigated as a substitute to DIOS chips to make the overall sample preparation simpler and safer [18]. Provided that the appropriate powder preparation was performed (including particle etching by HF, oxidation by HNO 3 , and derivatization of the generated SiOH groups), such silicon nanopowder method was found to exhibit the same characteristics as DIOS mass spectrometry.Porous silicon is a material that presents (1) a large active surface and (2) a strong absorbance in the UV range. Such a form of silicon was thus found particularly suitable for LDI mass spectrometry with irradiation at 337 nm, the pore size and the surface chemical nature being the most important parameters as stated by Siuzdak and coworkers in their early publications on DIOS [20...

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Section: Preliminary Ldi Analysesmentioning

confidence: 99%

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confidence: 99%

Laser desorption/ionization mass spectrometry on porous silica and alumina for peptide mass fingerprinting

Shenar

Martinez

Enjalbal

2008

J. Am. Soc. Mass Spectrom.

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“…The sputter atomization process in the glow discharge is similar to the release of sample species in SIMS and fast atom bombardment mass spectrometry (FABMS). 28 In SIMS and FABMS, the analytical signal carriers are that small fraction of sample species that are sputtered as secondary ions. This is not the case for GDMS because the strong negative electric bias applied to the target cathode rapidly returns secondary ions to the sample surface.…”

Section: Analyte Atomization and Ionizationmentioning

confidence: 99%

“…Promising results have been obtained from ion trap mass spectrometric systems by investigators at Oak Ridge National Laboratory41 and from time-of-Ñight mass spectrometric systems at Indiana University42 and West Virginia University. 28 The use of Fourier transform (FT) MS coupled with a glow discharge ion source was found to permit difficult analyses requiring great mass resolving power.43 At present, the commercial GDMS systems available for trace element analysis employ only double-focusing or quadrupole mass analyzers.…”

Section: Instrumentationmentioning

confidence: 99%

Special feature: Tutorial. Glow discharge mass spectrometry: Trace element determinations in solid samples

1995

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Glow discharge mass spectrometry (GDMS) is a mature, versatile technique for the direct determination of trace elements in a variety of materials. The technique is an extension of the earliest forms of mass spectrometry. Processes inherent to the glow discharge, namely cathodic sputtering coupled with Penning ionization, yield an ion population from which semi-quantitative results can be directly obtained. QuantiÐcation in GDMS is achieved both through standard elemental mass spectrometric procedures and more innovative approaches. The analytical performance of GDMS compares favorably with competing elemental mass spectrometric methods and newer experiments use this ionization method for both molecular and elemental analysis. As with any analytical technique, the future of GDMS lies in improvements with respect to instrumental implementation and extension to new areas of application. If the method is to remain competitive, commercial GDMS systems must incorporate advances in mass spectrometric technology to increase analytical performance while decreasing the size, complexity and cost of the technique. Continued e †orts to develop improved quantitation procedures are needed to provide greater accuracy. The method should continue to mature as sustained e †orts demonstrate its utility in the solution of new and more varied problems.

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“…This technique was called matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). In this technique, the matrix absorbs most of the laser energy and the resulting energisation leads to a phase transfer, ionization and formation of stable molecular ions 7 . Using MALDI-MS, it is possible to detect intact molecules having molecular weights greater than 200,000 u 8 .…”

Section: Introductionmentioning

confidence: 99%

Construction of a matrix assisted laser desorption/ionization time-of-flight mass spectrometer

et al. 2000

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A matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer has been constructed at the Department of Physics, University of Colombo, Sri Lanka. In this mass spectrometer, a solid sample which contains analyte molecules embedded in a suitable matrix compound in excess is exposed to a UV pulsed nitrogen laser source (λ = 337 nm). The desorbed secondary ions from the sample are mass analyzed using the time-of-flight technique. The first MALDI-TOF mass spectra of two peptides namely [tyr 8 ]-bradykinin (1076.2 u) and porcine N-acetyl renin substrate tetradecapeptide (1801.1 u) with the matrix 2,5 di-hydro benzoic acid (154.1 u) have been obtained. Each peptide produced a MH + peak corresponding to its intact molecular ion with m/q values of 1089 ± 6 and 1794 ± 8 respectively.

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Special feature: Tutorial. Desorption ionization mass spectrometry

Cited by 72 publications

References 20 publications

Laser desorption/ionization mass spectrometry on porous silica and alumina for peptide mass fingerprinting

Laser desorption/ionization mass spectrometry on porous silica and alumina for peptide mass fingerprinting

Special feature: Tutorial. Glow discharge mass spectrometry: Trace element determinations in solid samples

Construction of a matrix assisted laser desorption/ionization time-of-flight mass spectrometer

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