Abstract:Lysophosphatidylcholine acyltransferase‐1 (LPCAT1) plays a critical role in the remodeling of phosphatidylcholines (PCs) in cellular lipidome. However, evidence is scarce regarding its sn‐selectivity, viz. the preference of assembling acyl‐Coenzyme A (CoA) at the C1 or C2‐hydroxyl on a glycerol backbone because of difficulty to quantify the thus‐formed PC sn‐isomers. We have established a multiplexed assay to measure both sn‐ and acyl‐chain selectivity of LPCAT1 toward a mixture of acyl‐CoAs by integrating iso… Show more
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