Semecarpus anacardium L.f. leaf extract exhibits activities against breast cancer and prolongs the survival of tumor-bearing mice

Abstract

Semecarpus anacardium L.f. has been commonly used in various traditional medicines from ancient times. The nuts have been described in Ayurveda medication systems to treat numerous clinical ailments. However, isolating phytochemical constituents from nuts remains challenging and exhibits cytotoxic effects on other cells. In this study, we have standardized procedures for isolating phytochemicals from the leaf extract. The ethyl acetate leaf extract selectively affects cancer cells in a dose-dependent manner (IC50: 0.57 µg/ml in MCF-7 cells) in various cancer cell lines and induces apoptosis in cancer cells. However, the non-malignant cells were relatively insensitive to the extract.Next, the incubation of the leaf extract induces cell cycle arrest and suppresses cancer cell migration in the cell culture model. Moreover, oral administration of extract significantly restored tumor growth in mice. Together, these observations suggest the anti-cancer activities of S. anacardium L.f. leaf potential for both in vitro and in vivo models.

Antioxident and Anticancer Potency of Hydro-Alcoholic Ingudi (Balanites aegyptiaca Linn. Delile) Seed Extract

“…2) quanti ed by image J this may be due to the presence of phytochemicals present in the plant extract that inhibit the migratory ability of breast cancer cells. A similar result was observed by Hsu et al,2015 where the breast cancer cells' wound area was signi cantly larger than to control with the increasing dose of betulin [14].…”

“…Then, the effect of plant extract on human peripheral blood mononuclear cells (PBMCs) was evaluated, using human blood, which was obtained from the volunteers with pre-informed consent. The blood was centrifuged to get peripheral blood mononuclear cells (PBMCs), followed by PBMCs isolation from the blood using density gradient centrifugation method and seeded into 96 well plates using RPMI-1640 media, supplemented with 10% FBS and 1% antibiotic-antimycotic solution, whereas MCF-7 cells were cultured in MEM medium containing 10% fetal bovine serum, MDA-MB-231 cells were cultured in Leibovitz-15 (L-15 medium containing 10% FBS, 100 U/mL penicillin, and 100mg/L streptomycin [14,15].…”