<i>Selenomonas</i>: A marker of asthma severity with the potential therapeutic effect

Kim, Young-Chan; Choi, Sung-Mi; Sohn, Kyung Hee; Bang, Ji‐Young; Kim, Yuldam; Jung, Jae-Woo; Kim, Hye-Young; Park, Jaehyun; Kim, Kangjin; Kang, Min‐Gyu; Yang, Min‐Suk; Kim, Sujeong; Kim, Sae‐Hoon; Choi, Jeong‐Hee; Park, Hye Jung; Lee, Sang Min; Kim, Sang Heon; Park, Jung-Won; Lee, Jong Myung; Cho, Sang Heon; Won, Sungho; Yi, Hana; Kang, Hye–Ryun

doi:10.1111/all.15114

Cited by 5 publications

(6 citation statements)

References 6 publications

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“…Fibrobacter was the most active cellulolytic bacterium known, producing succinic acid as its main fermentation product [18]. Thus, reduction in Selenomonas is a marker of asthma severity [19]. Butyrivibrio spp.…”

Section: Discussionmentioning

confidence: 99%

Respiratory syncytial virus infection disrupts pulmonary microbiota and immune barriers to induce microglia phenotype shift

Tan

Bajinka

Tang

et al. 2023

Preprint

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Background: The lung-brain-axis is an emerging biological pathway that is being investigated in relation to microbiome medicine. Increasing evidence suggest that pulmonary viral infections can lead to distinct pathological imprints in the brain, thereby the need to explore and understand this mechanism and find possible interventions. Objective: This study used RSV infection in mice as a model to establish the potential lung-brain axis phenomenon. We hypothesized that RSV infection could disrupt the lung microbiota, thereby compromising the immune barriers and thus induces significant shift in microglia phenotype. Methods: Mice were randomized into the Control, Ampicillin, RSV, and RSV+Ampicillin treated groups (n = 6 each). Ampicillin was given intratracheal instillation and seven days after the respective treatments, the mice were anesthetized. Hematoxylin-eosin (HE) staining of lung tissue to detect histopathology. Immunofluorescence label of specific target antigens in both the lung and brain tissues, namely, Malondialdehyde (MDA) and Superoxide dismutase (SOD) were used as markers of cellular damage. RT-qPCR was used to detect viral RNA in both tissues, ELISA to measure IL-1β, iNOS, IL-10 and Arg1 in the supernatant and 16s DNA technology were used to detect the lung microflora. Results: We found out that RSV infection induces elevated oxidative stress, reduced anti-oxidant and caused significant dysbacteriosis in the lungs of the mice. Pulmonary microbes were found affecting Th1-type immunoreactivity induced by RSV infection and eventually, microbiota in lung induced microglia phenotype shift in the brain of the mice. Conclusion. This study was able to establish that RSV infection can disrupt the pulmonary microbiome and immune barriers to induce microglia phenotype shift. Thus, we recommend a large sample size study with robust data analysis for the long-term effects of antibiotics and RSV infection on brain physiology.

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Section: Discussionmentioning

confidence: 99%

Respiratory syncytial virus infection disrupts pulmonary microbiota and immune barriers to induce microglia phenotype shift

Tan

Bajinka

Tang

et al. 2023

Preprint

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show abstract

“…Fibrobacter was the most active cellulolytic bacterium known, producing succinic acid as its main fermentation product 26 . Thus, reduction in Selenomonas is a marker of asthma severity 27 . Butyrivibrio is one of the symbiotic bacteria that can produce short‐chain fatty acids, especially butyrate.…”

Section: Discussionmentioning

confidence: 99%

“…26 Thus, reduction in Selenomonas is a marker of asthma severity. 27 Butyrivibrio is one of the symbiotic bacteria that can produce short-chain fatty acids, especially butyrate. Following Ampicillin administration, the number of Bifidobacterium was further reduced compared to the RSV group.…”

Section: Rsv Infection Induced Pulmonary Dysbacteriosismentioning

confidence: 99%

Respiratory syncytial virus infection disrupts pulmonary microbiota to induce microglia phenotype shift

Bajinka

Tang

Mao

et al. 2023

Journal of Medical Virology

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The lung−brain axis is an emerging biological pathway that is being investigated in relation to microbiome medicine. Increasing evidence suggests that pulmonary viral infections can lead to distinct pathological imprints in the brain, so there is a need to explore and understand this mechanism and find possible interventions. This study used respiratory syncytial virus (RSV) infection in mice as a model to establish the potential lung−brain axis phenomenon. We hypothesized that RSV infection could disrupt the lung microbiota, compromise immune barriers, and induce a significant shift in microglia phenotype. One week old mice were randomized into the control, Ampicillin, RSV, and RSV+Ampicillin treated groups (n = 6 each). Seven days after the respective treatments, the mice were anaesthetized. Immunofluorescence and real‐time qRT‐PCR was used to detect virus. Hematoxylin‐eosin staining was used to detect histopathology. Malondialdehyde and superoxide dismutase were used to determine oxidative stress and antioxidant capacity. Real‐time qRT‐PCR and enzyme‐linked immunosorbent assay (ELISA) were used to measure Th differentiation in the lung. Real‐time qRT‐PCR, ELISA, and confocal immunofluorescence were used to determine the microglia phenotype. 16S DNA technology was used to detect lung microflora. RSV infection induces elevated oxidative stress, reduced antioxidant, and significant dysbacteriosis in the lungs of mice. Pulmonary microbes were found to enhance Th1‐type immunoreactivity induced by RSV infection and eventually induced M1‐type dominant microglia in the brains of mice. This study was able to establish a correlation between the pulmonary microbiome and brain function. Therefore, we recommend a large sample size study with robust data analysis for the long‐term effects of antibiotics and RSV infection on brain physiology.

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“…1 Recent studies have demonstrated a diverse airway microbiome associated with asthma severity and exacerbation. 2,3 There is also growing evidence of the bidirectional immune interactions between the gut microbiome and respiratory mucosa known as the gut-lung axis. 4 While gut microbiome dysbiosis in early life may influence asthma susceptibility, 5,6 little is known about the microbiome enterotypes of asthma in adults.…”

Section: Different Inflammatory Features Of Asthma According To Gut M...mentioning

confidence: 99%

“…Kyoung-Hee Sohn 1,2 Sungmi Choi 3 Jae-Woo Jung 4 Jeong-Hee Choi 5 Sang-Heon Cho 1,6 Hana Yi 3,7 Hye-Ryun Kang…”

Section: Fu N D I N G I N Fo R M Ati O Nunclassified

Different inflammatory features of asthma according to gut microbiome enterotype

Sohn

Choi

Jung

et al. 2023

Allergy

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Selenomonas: A marker of asthma severity with the potential therapeutic effect

Cited by 5 publications

References 6 publications

Respiratory syncytial virus infection disrupts pulmonary microbiota and immune barriers to induce microglia phenotype shift

Respiratory syncytial virus infection disrupts pulmonary microbiota and immune barriers to induce microglia phenotype shift

Respiratory syncytial virus infection disrupts pulmonary microbiota to induce microglia phenotype shift

Different inflammatory features of asthma according to gut microbiome enterotype

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