<i><scp>P</scp>lum pox virus</i> and sharka: a model potyvirus and a major disease

Garcı́a, Juan Antonio; Glasa, Miroslav; Cambra, M.; Candresse, Thierry

doi:10.1111/mpp.12083

Cited by 186 publications

(153 citation statements)

References 250 publications

(288 reference statements)

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“…In ELISA the viral CP can be assayed. Most virus specific and strain specific epitopes are located at N-end of CP, which is exposed on the surface of the viral particle being the most variable part of the molecule [9]. Numerous assay kits for PPV diagnostics based on sandwich-ELISA with polyclonal antibodies and conjugated alkaline phosphatase are well represented on the market.…”

Section: Fig 2 Dendrogram Of Plum Pox Virus (Ppv)mentioning

confidence: 99%

“…These are Dideron (D), Marcus (M), Recombinant (Rec), Cherry (С), Cherry Russian (CR), El Amar (EA), Winona (W), Turkish (T) and Ancestral (An) [9,13], clustered into monophylic groups of closely related isolates. Recombination plays important role in the PPV evolution together with spot mutations.…”

Section: Fig 2 Dendrogram Of Plum Pox Virus (Ppv)mentioning

confidence: 99%

“…Recombination plays important role in the PPV evolution together with spot mutations. M and T probably appeared due to independent recombination between isolates of D and An strains [9]. Rec strain considered to be a recombinant of D and M isolates [14].…”

Section: Fig 2 Dendrogram Of Plum Pox Virus (Ppv)mentioning

confidence: 99%

“…For long distances PPV can be mainly transmitted via infected plants. The disease is recorded all around the world, except Australia, New Zeeland, South Africa and California [9][10][11]. In Russia PPV is a limitedly spread quarantine virus.…”

mentioning

confidence: 99%

“…In infected cells RNA is expressed into single precursor polyprotein than processed by virus-specific proteases into 10 functional proteins (P1, HcPro, P3, 6K1, CI, 6K2, VPg, NIa-Pro, NIb and capsid protein CP). Another protein, the PIPO (Pretty Interesting Potiviridae ORF), is expressed due to the fusion of P3N and PIPO resulting from the shift in the P3 gene reading frame [9].…”

mentioning

confidence: 99%

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Genetic Diversity and Population Structure

Chirkov¹,

Prikhod’ko²

2015

S-h. biol.

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A b s t r a c t Plum pox virus (PPV) is the causal agent of Sharka that is considered the most detrimental viral disease of stone fruit crops. Regular monitoring of stone fruit plantings using enzyme-linked immunosorbent assay and reverse-transcription polymerase chain reaction for PPV detection and identification resulted in the findings of numerous focuses of the disease in European Russia. The virus was found in collections, variety test plots, nurseries, fructiferous and abandoned orchards, decorative plantings, private gardens, wild stone fruit trees growing in urban and rural areas. PPV was detected in naturally infected plum (Prunus domestica), peach (P. persica), nectarine (P. persica var. nectarina), myrobalan (P. cerasifera), blackthorn (P. spinosa), downy cherry (P. tomentosa), sour cherry (P. cerasus), sweet cherry (P. avium), apricot (P. armeniaca) and Canadian plum (P. nigra). PPV has been reported from Petersburg, Novgorod, Tver, Moscow, Tula, Voronezh, Tambov, Lipetsk, Belgorod, Rostov, Samara, Saratov, Volgograd, Astrakhan, Stavropol, Krasnodar, Karachay-Cherkessia, Dagestan, and Crimea regions. Six of the nine known PPV strains (D, M, Rec, W, C, CR) have been revealed in European Russia. Most isolates belong to the strains D (38 %), W (25 %), CR (23 %), M (7 %) and C (7 %). Two distinct PPV-Rec isolates have been found on myrobalan and plum in Crimea and Stavropol regions. Population of PPV in European Russia and, probably, all over the European part of the former USSR seems to be the most diverse in the world due to wide spread of PPV isolates belonging to the strains W, C, and CR that were never detected or only sporadically identified in other countries until now. Phylogenetic analysis of their genomes shows that these three strains constitute the supercluster divergent from other PPV strains. This evolutionary branch originated from a common ancestor and apparently developed mainly in Russia. The wide dissemination of PPV in Russia is a potential threat for newly bred stone fruit cultivars and further selection and biotechnological works.

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Section: Fig 2 Dendrogram Of Plum Pox Virus (Ppv)mentioning

confidence: 99%

Section: Fig 2 Dendrogram Of Plum Pox Virus (Ppv)mentioning

confidence: 99%