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              <scp>M</scp>
              ethylobacter
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Collins, Dave; Akberdin, Ilya R.; Kalyuzhnaya, Marina G.

doi:10.1002/9781118960608.gbm01179.pub2

Cited by 5 publications

(1 citation statement)

References 32 publications

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“…The genus Methylobacter, belonging to the family Methylomonadaceae (Type Ia), was initially proposed by Bowman and collaborators in 1993 (Bowman et al 1993 ). Currently, the genus now contains 8 validly published species (Collins et al 2015 and 2017 ). All members of the genus Methylobacter are strictly aerobic, rod-shaped, and capable of aerobically oxidizing methane to carbon dioxide.…”

Section: Introductionmentioning

confidence: 99%

Targeting methanotrophs and isolation of a novel psychrophilic Methylobacter species from a terrestrial Arctic alkaline methane seep in Lagoon Pingo, Central Spitsbergen (78° N)

Patil,

Islam,

Tveit

et al. 2024

Antonie van Leeuwenhoek

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The microbial diversity associated with terrestrial groundwater seepage through permafrost soils is tightly coupled to the geochemistry of these fluids. Terrestrial alkaline methane seeps from Lagoon Pingo, Central Spitsbergen (78°N) in Norway, with methane-saturated and oxygen-limited groundwater discharge providing a potential habitat for methanotrophy. Here, we report on the microbial community’s comparative analyses and distribution patterns at two sites close to Lagoon Pingo’s methane emission source. To target methane-oxidizing bacteria from this system, we analysed the microbial community pattern of replicate samples from two sections near the main methane seepage source. DNA extraction, metabarcoding and subsequent sequencing of 16S rRNA genes revealed microbial communities where the major prokaryotic phyla were Pseudomonadota (42–47%), Gemmatimonadota (4–14%) and Actinobacteriota (7–11%). Among the Pseudomonadota, members of the genus Methylobacter were present at relative abundances between 1.6 and 4.7%. Enrichment targeting the methane oxidising bacteria was set up using methane seep sediments as inoculum and methane as the sole carbon and energy source, and this resulted in the isolation of a novel psychrophilic methane oxidizer, LS7-T4AT. The optimum growth temperature for the isolate was 13 °C and the pH optimum was 8.0. The morphology of cells was short rods, and TEM analysis revealed intracytoplasmic membranes arranged in stacks, a distinctive feature for Type I methanotrophs in the family Methylomonadaceae of the class Gammaproteobacteria. The strain belongs to the genus Methylobacter based on high 16S rRNA gene similarity to the psychrophilic species of Methylobacter psychrophilus Z-0021T (98.95%), the psychrophilic strain Methylobacter sp. strain S3L5C (99.00%), and the Arctic mesophilic species of Methylobacter tundripaludum SV96T (99.06%). The genome size of LS7-T4AT was 4,338,157 bp with a G + C content of 47.93%. The average nucleotide identities (ANIb) of strain LS7-T4AT to 10 isolated strains of genus Methylobacter were between 75.54 and 85.51%, lower than the species threshold of 95%. The strain LS7-T4AT represents a novel Arctic species, distinct from other members of the genus Methylobacter, for which the name Methylobacter svalbardensis sp. nov. is proposed. The type of strain is LS7-T4AT (DSMZ:114308, JCM:39463).

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Section: Introductionmentioning

confidence: 99%

Targeting methanotrophs and isolation of a novel psychrophilic Methylobacter species from a terrestrial Arctic alkaline methane seep in Lagoon Pingo, Central Spitsbergen (78° N)

Patil,

Islam,

Tveit

et al. 2024

Antonie van Leeuwenhoek

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Community Structure of Active Aerobic Methanotrophs in Red Mangrove (Kandelia obovata) Soils Under Different Frequency of Tides

et al. 2017

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Methanotrophs are important microbial communities in coastal ecosystems. They reduce CH emission in situ, which is influenced by soil conditions. This study aimed to understand the differences in active aerobic methanotrophic communities in mangrove forest soils experiencing different inundation frequency, i.e., in soils from tidal mangroves, distributed at lower elevations, and from dwarf mangroves, distributed at higher elevations. Labeling of pmoA gene of active methanotrophs using DNA-based stable isotope probing (DNA-SIP) revealed that methanotrophic activity was higher in the dwarf mangrove soils than in the tidal mangrove soils, possibly because of the more aerobic soil conditions. Methanotrophs affiliated with the cluster deep-sea-5 belonging to type Ib methanotrophs were the most dominant methanotrophs in the fresh mangrove soils, whereas type II methanotrophs also appeared in the fresh dwarf mangrove soils. Furthermore, Methylobacter and Methylosarcina were the most important active methanotrophs in the dwarf mangrove soils, whereas Methylomonas and Methylosarcina were more active in the tidal mangrove soils. High-throughput sequencing of the 16S ribosomal RNA (rRNA) gene also confirmed similar differences in methanotrophic communities at the different locations. However, several unclassified methanotrophic bacteria were found by 16S rRNA MiSeq sequencing in both fresh and incubated mangrove soils, implying that methanotrophic communities in mangrove forests may significantly differ from the methanotrophic communities documented in previous studies. Overall, this study showed the feasibility of CH DNA-SIP to study the active methanotrophic communities in mangrove forest soils and revealed differences in the methanotrophic community structure between coastal mangrove forests experiencing different tide frequencies.

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Comparison of the gut microbiota in the groundwater amphipod Crangonyx islandicus Svavarsson & Kristjánsson, 2006 (Amphipoda: Crangonyctidae) to biofilms in its spring-source habitat

Guðmundsdóttir

Björnsdóttir

Marteinsson

et al. 2020

Journal of Crustacean Biology

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Crangonyx islandicus Svavarsson & Kristjánsson, 2006 is a groundwater amphipod endemic to Iceland and restricted to spring sources at edges of lava fields in the volcanically active zone. Genetic analysis indicate that the amphipod has survived in subglacial refugia in the groundwater in Iceland during past Ice Ages. We investigated the gut bacteria of C. islandicus and compared them with the bacterial diversity identified in biofilms from its groundwater habitat. The diversity of both uncultivated and cultivated bacteria was analyzed using partial 16S rRNA gene sequences. The results show that iron-oxidizing bacteria such as Sideroxydans and Gallionella and the methanotroph Methylobacter are common in the biofilm but absent from the gut samples. Thiomonas was common in both the biofilm and the gut samples. The cultivation of gut bacteria revealed that most of the taxa could be linked with terrestrial soil and/or freshwater habitat based on available database entries. This indicates that the food source for the amphipods is partly of terrestrial origin. The presence of primary producers in the subsurface habitat of C. islandicus indicate that microbial chemolithotrophic activity might fuel the system at least partly and thus explain the amphipod survival in the subsurface of Iceland during repeated glaciation for the past 5 mya.

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M ethylobacter

Cited by 5 publications

References 32 publications

Targeting methanotrophs and isolation of a novel psychrophilic Methylobacter species from a terrestrial Arctic alkaline methane seep in Lagoon Pingo, Central Spitsbergen (78° N)

Targeting methanotrophs and isolation of a novel psychrophilic Methylobacter species from a terrestrial Arctic alkaline methane seep in Lagoon Pingo, Central Spitsbergen (78° N)

Community Structure of Active Aerobic Methanotrophs in Red Mangrove (Kandelia obovata) Soils Under Different Frequency of Tides

Comparison of the gut microbiota in the groundwater amphipod Crangonyx islandicus Svavarsson & Kristjánsson, 2006 (Amphipoda: Crangonyctidae) to biofilms in its spring-source habitat

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