<i>
              <scp>L</scp>
              imnohabitans
            </i>

Kasalický, Vojtěch

doi:10.1002/9781118960608.gbm01537

Cited by 3 publications

(1 citation statement)

References 46 publications

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“…Such bio lm-forming bacteria clearly dominate all solid substrates assessed and could potentially serve as the main protective structure for other bio lm colonisers and even organisms that typically have a planktonic lifestyle. For example, the typically planktonic Burkholderiales genus Limnohabitans (Kasalický, 2019) was detected on plastic (LDPE and W-LDPE) and wood (averaging 11, 12 and 13%, respectively), although it was much more abundant in the surrounding water (44%; Fig. 1C).…”

Section: Resultsmentioning

confidence: 99%

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

Zadjelovic,

Wright,

Borsetto

et al. 2023

Preprint

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Background The widespread nature of plastic pollution has given rise to wide scientific and social concern regarding the capacity of these materials to serve as vectors for pathogenic bacteria and reservoirs for Antimicrobial Resistance Genes (ARG). In- and ex-situ were used to characterise the riverine plastisphere taxonomically and functionally in order to determine whether antibiotics within the water influenced the ARG profiles in these microbiomes and how these compared to those on natural surfaces such as wood and their planktonic counterparts. Results We show that plastics support a taxonomically distinct microbiome containing potential pathogens and ARGs. While the plastisphere was similar to those biofilms that grew on wood, they were distinct from the surrounding water microbiome. Hence, whilst potential opportunistic pathogens (i.e. Pseudomonas aeruginosa, Acinetobacter and Aeromonas) and ARG subtypes (i.e. those that confer resistance to macrolides/lincosamides, rifamycin, sulfonamides, disinfecting agents and glycopeptides) were predominant in all surface-related microbiomes, especially on weathered plastics, a completely different set of potential pathogens (i.e. Escherichia, Salmonella, Klebsiella and Streptococcus) and ARGs (i.e. aminoglycosides, tetracycline, aminocoumarin, fluoroquinolones, nitroimidazole, oxazolidinone and fosfomycin) dominated in the planktonic compartment. Our genome-centric analysis allowed the assembly of 215 Metagenome Assembled Genomes (MAGs), linking ARGs and other virulence-related genes to their host. Interestingly, a MAG belonging to Escherichia –that clearly predominated in water– harboured more ARGs and virulence factors than any other MAG, emphasising the potential virulent nature of these pathogenic-related groups. Finally, ex-situ incubations using environmentally-relevant concentrations of antibiotics increased the prevalence of their corresponding ARGs, but different riverine compartments –including plastispheres– were affected differently by each antibiotic. Conclusions Our results provide insights into the capacity of the riverine plastisphere to harbour a distinct set of potentially pathogenic bacteria and function as a reservoir of ARGs. The environmental impact that plastics pose if they act as a reservoir for either pathogenic bacteria or ARGs is aggravated by the persistence of plastics in the environment due to their recalcitrance and buoyancy. Nevertheless, the high similarities with microbiomes growing on natural co-occurring materials and even more worrisome microbiome observed in the surrounding water highlights the urgent need to integrate the analysis of all environmental compartments when assessing risks and exposure to pathogens and ARGs in anthropogenically-impacted ecosystems.

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Section: Resultsmentioning

confidence: 99%

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

Zadjelovic,

Wright,

Borsetto

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

Zadjelovic,

Wright,

Borsetto

et al. 2023

Microbiome

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Background The widespread nature of plastic pollution has given rise to wide scientific and social concern regarding the capacity of these materials to serve as vectors for pathogenic bacteria and reservoirs for Antimicrobial Resistance Genes (ARG). In- and ex-situ incubations were used to characterise the riverine plastisphere taxonomically and functionally in order to determine whether antibiotics within the water influenced the ARG profiles in these microbiomes and how these compared to those on natural surfaces such as wood and their planktonic counterparts. Results We show that plastics support a taxonomically distinct microbiome containing potential pathogens and ARGs. While the plastisphere was similar to those biofilms that grew on wood, they were distinct from the surrounding water microbiome. Hence, whilst potential opportunistic pathogens (i.e. Pseudomonas aeruginosa, Acinetobacter and Aeromonas) and ARG subtypes (i.e. those that confer resistance to macrolides/lincosamides, rifamycin, sulfonamides, disinfecting agents and glycopeptides) were predominant in all surface-related microbiomes, especially on weathered plastics, a completely different set of potential pathogens (i.e. Escherichia, Salmonella, Klebsiella and Streptococcus) and ARGs (i.e. aminoglycosides, tetracycline, aminocoumarin, fluoroquinolones, nitroimidazole, oxazolidinone and fosfomycin) dominated in the planktonic compartment. Our genome-centric analysis allowed the assembly of 215 Metagenome Assembled Genomes (MAGs), linking ARGs and other virulence-related genes to their host. Interestingly, a MAG belonging to Escherichia –that clearly predominated in water– harboured more ARGs and virulence factors than any other MAG, emphasising the potential virulent nature of these pathogenic-related groups. Finally, ex-situ incubations using environmentally-relevant concentrations of antibiotics increased the prevalence of their corresponding ARGs, but different riverine compartments –including plastispheres– were affected differently by each antibiotic. Conclusions Our results provide insights into the capacity of the riverine plastisphere to harbour a distinct set of potentially pathogenic bacteria and function as a reservoir of ARGs. The environmental impact that plastics pose if they act as a reservoir for either pathogenic bacteria or ARGs is aggravated by the persistence of plastics in the environment due to their recalcitrance and buoyancy. Nevertheless, the high similarities with microbiomes growing on natural co-occurring materials and even more worrisome microbiome observed in the surrounding water highlights the urgent need to integrate the analysis of all environmental compartments when assessing risks and exposure to pathogens and ARGs in anthropogenically-impacted ecosystems.

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技術が拓く湖沼微生物生態学の最前線

OKAZAKI

2024

Japanese Journal of Limnology (Rikusuigaku Zasshi)

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In microbial ecology, technical challenges owing to the small cell size of organisms have been a bottleneck; however, new technologies have directly led to discoveries and breakthroughs, paving the way for novel insights. This review examines recent developments in lake microbial ecology, with a particular focus on their technological foundations and practical considerations for application in lakes. Amplicon sequencing and metagenomic analyses, which have emerged with the development of high-throughput DNA sequencing and analysis technologies, have revolutionized our understanding of the diversity and ecology of microbes that are difficult to culture in various environments. The significance of cell counting and microscopy, such as fluorescent in situ hybridization, has increased as nucleotide sequence data have become more comprehensive. Isolating difficult-to-culture microorganisms has also become a means of complementing information and testing the hypotheses obtained from sequencing data. We are now in an era in which we can determine "who is doing what" within complex microbial ecosystems at a high resolution, dispelling the notion that microbial ecosystems are "black boxes." The diverse expertise and approaches offered by the framework of limnology are key to making the best use of this vast amount of data.

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L imnohabitans

Cited by 3 publications

References 46 publications

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

Microbial hitchhikers harbouring antimicrobial-resistance genes in the riverine plastisphere

技術が拓く湖沼微生物生態学の最前線

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