HLA‐DRB1*15:01:43 and HLA‐DRB1*15:01:44 alleles were identified by next‐generation sequencing

Abstract

submitted to the IPD-IMGT/HLA Database 2 in December 2021. This follows the agreed policy that, subject to conditions stated in the most recent Nomenclature Report, 3 names will be assigned to new sequences as they are identified. Lists of such names will be published in the following WHO Nomenclature Report. ACKNOWLEDGMENTSThe authors would like to offer special gratitude to the volunteer donors for their participation in our bone marrow donor registry. We would also like to thank Dharma Master Cheng Yen, founder of the Buddhist Compassion Relief Tzu Chi Foundation, for continuing support and kind encouragement both spiritually and intellectually. Furthermore, the generosity and camaraderie that our colleagues bestow on us are much appreciated.

Identification of two novel HLA‐DRB1 alleles, HLA‐DRB1*08:03:13 and HLA‐DRB1*08:119

“…HLA‐DRB1*08:03:13 allele was found in the donor from the Cord Blood Bank of Zhejiang Province, China. The sample was genotyped for HLA‐A, ‐B, ‐C, ‐DRB1, ‐DQB1, and ‐DPB1 loci by NGS with AllType™ 11‐loci kit (One Lambda Inc., Canoga Park, CA) as previously reported 2,3 . HLA‐DRB1*12:02 allele and a novel allele, now named as HLA‐DRB1*08:03:13 were identified in the sample.…”

“…The sample was genotyped for HLA-A, -B, -C, -DRB1, -DQB1, and -DPB1 loci by NGS with AllType™ 11-loci kit (One Lambda Inc., Canoga Park, CA) as previously reported. 2,3 HLA-DRB1*12:02 allele and a novel allele, now named as HLA-DRB1*08:03:13 were identified in the sample. The sequence of exons 2 to 6 of the HLA-DRB1*08:03:13 allele was identical to that of the HLA-DRB1*08:03:02:01 allele, except for one nucleotide change at position 309 G>A in exon 2, which is a synonymous change that does not lead to amino acid change (Figure 1 The names HLA-DRB1*08:03:13 and HLA-DRB1*08 :119 have been officially assigned by the WHO Nomenclature Committee for Factors of the HLA System in January 2022 and December 2022 respectively.…”

Description of the novel HLA allele, HLA‐C*01:220 in a Chinese individual

“…The proband comes from the Cord Blood Bank of Zhejiang Province, China. The sample of the proband was firstly genotyped for the HLA‐A, ‐B, ‐C, ‐DRB1, and ‐DQB1 loci at high resolution level using the AllType™ NGS 11‐loci amplification kit (One Lambda Inc., Canoga Park, CA, USA) and the genotype of the sample was assigned with TypeStream Visual Software version 2.0(One Lambda Inc.) as previously reported 2–4 …”

“…The sample of the proband was firstly genotyped for the HLA-A, -B, -C, -DRB1, and -DQB1 loci at high resolution level using the AllType™ NGS 11-loci amplification kit (One Lambda Inc., Canoga Park, CA, USA) and the genotype of the sample was assigned with TypeStream Visual Software version 2.0 (One Lambda Inc.) as previously reported. [2][3][4] The novel allele HLA-C*01:220 was identical to HLA-C*01:02:01:01 except for one single nucleotide substitution A to T at position 559 in exon 3, which resulted in an amino acid change from Threonine to Serine at residue 163 (Figure 1). The accession number OK106260 in the GenBank nucleotide sequence database was assigned for the nucleotide sequence of the HLA-C*01:220 allele.…”

Identification of the novel HLA‐DRB1*11:298 allele in a Chinese cord blood donor

“…The HLA-A, -C, -B, -DRB1, -DQB1 and -DPB1 loci of the blood donor from the Cord Blood Bank of Zhejiang Province, China was genotyped using the AllType™ NGS 11-loci amplification kit (One Lambda Inc., Canoga Park, CA, USA) and the genotype of the donor was assigned with the TypeStream Visual Software version 2.0 (One Lambda Inc.) as previously reported. 2,3 The sequence of HLA-DRB1*11:298 allele differs from the HLA-DRB1*11:01:01:01 allele by one single nucleotide substitution at position 397 T>G in The name HLA-DRB1*11:298 has been officially assigned by the World Health Organization Nomenclature Committee for Factors of the HLA System in January 2022. This follows the agreed policy that, subject to the conditions stated in the most recent Nomenclature report, 4 names will be assigned to new sequences as they are identified.…”