I<scp>G</scp>E AND I<scp>G</scp>GA ANTIBODY-MEDIATED RELEASE OF HISTAMINE FROM RAT PERITONEAL CELLS

Bach, Michael K.; Bloch, Kurt J.; Austen, K. Frank

doi:10.1084/jem.133.4.752

Cited by 78 publications

(25 citation statements)

References 23 publications

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“…These effects are quantitatively comparable to the results reported by Levy and Osler on human basophils (14). We did not observe the enhancing effect of Ca ++ which Bach et al's data on rat mast cells implied (16).…”

Section: Discussioncontrasting

confidence: 77%

“…5 No significant effect of pH on kl and k ~ was observed in the range 6-8 (Table III). Levy and Osler (14), using human basophils, and Bach et al (16), using rat mast cells, reported marked optima at pH 7.4 and 6.8 respectively. It is uncertain whether the pH effects on sensitization observed by them were directly related to the reagin-cell interaction or to some other factors.…”

Section: Discussionmentioning

confidence: 99%

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THE INTERACTION OF IgE WITH RAT BASOPHILIC LEUKEMIA CELLS

Kulczycki

Metzger

1974

The Journal of Experimental Medicine

304

106

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W e h a v e d e m o n s t r a t e d t h a t r a t b a s o p h i l i c l e u k e m i a cells ( R B L -1 cells) 1 b i n d rat I g E (1). T h o s e s t u d i e s a n d o t h e r s (2) s h o w e d t h a t t h e I g E is b o u n d to t h e cell s u r f a c e w i t h a s p e c i f i c i t y s i m i l a r to t h a t o b s e r v e d w i t h n o r m a l rat m a s t cells.In t h i s p a p e r we p r e s e n t a q u a n t i t a t i v e a n a l y s i s of t h e b i n d i n g . F o r s o m e of t h e e x p e r i m e n t s larger a m o u n t s of I g E were n e c e s s a r y t h a n c o u l d be o b t a i n e d easily f r o m r e a g i n i c s e r u m . F o r t h i s reason an I g E m y e l o m a p r o t e i n (3) was u s e d a f t e r d e m o n s t r a t i n g t h a t its b i n d i n g p r o p e r t i e s were i n d i s t i n g u i s h a b l e f r o m those o b s e r v e d for t h e r e a g i n i c a n t i b o d i e s u s e d p r e v i o u s l y . Materials and MethodsIgE Preparations. IgE was purified from rat anti-Nippostrongylus brasiliensis serum by the method described previously (4). A rat IgE myeloma protein was purified from serum of rats bearing tumor IR 162 (3) in an analogous fashion: 12 ml serum were dialyzed vs. 0.2 M borate-buffered saline pH 8.0 (BBS) and the protein precipitating between 38 and 48% saturated (NH4)2SO4 at 4°C collected. This fraction was chromatographed on a 4.1 × 105 cm column of Sepharose 6B and the major component in the effluent concentrated by ultrafiltration (Amicon Corp., Lexington, Mass.) and dialyzed against a solution which was 33 mM in Tris base, 25 mM in phosphate, having a conductivity of 2.3 mmho and a pH of 8.0 at room temperature. The protein (~ 100 absorbancy units at 280 nm) was next chromatographed on a 2.6 × 7.2 cm column of "DE-52" diethylaminoethyl cellulose (Whatman Biochemicals Ltd., Maidstone, England). The excluded component was dialyzed against BBS and applied successively to two Sepharose 2B immunoadsorbents containing rabbit antibodies to the normal rat serum fraction precipitating at 50% saturated (NH4)2S04 and rabbit antibodies to normal rat proteins excluded from DE-52 respectively (4). Both antibody preparations had been absorbed with a Sepharose 2B immunoadsorbent containing Fab fragments of rat IgG. The purified IgE preparations were stored at 4°C in BBS and radiolabeled with ~25I {New England Nuclear, Boston, Mass.) by the chloramine-T method (4, 5) as required.RBL-1 Cells. Cells were cultured in Falcon flasks (Falcon Plastics, Div. of BioQuest, Oxnard, Calif.) as described previously (1) or in spinner cultures (footnote 2). In the latter case the medium

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Section: Discussioncontrasting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

THE INTERACTION OF IgE WITH RAT BASOPHILIC LEUKEMIA CELLS

Kulczycki

Metzger

1974

The Journal of Experimental Medicine

304

106

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“…IgGa and IgE are immunochemically distinct antibodies with different functional char acteristics but homocytotropic, and both induce the mediator release from the mast cell (28,29). Furthermore, a common receptor or closely interacting receptors have been suggested as such would interfere with each other in the rat PCA reaction and in the mediator release from the sensitized rat peritoneal mast cell provoked by antigen (30,31).…”

Section: Methodsmentioning

confidence: 99%

Antiallergic action of 6-ethyl-3-(1h-tetrazol-5-yl) chromone (aa-344) on immediate hypersensitivity reaction in rats

Kuriki

Saijo

Maki

et al. 1979

Japanese Journal of Pharmacology

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Abstract-A newly synthesized compound, 6-ethyl-3-(IH-tetrazol-5-yl)chromone (AA-344) given intravenously or orally inhibited considerably the 72-hr passive cutaneous anaphylaxis (72-hr PCA) induced by IgE in rats. The antiallergic action of AA-344 was neither due to the antihistamine or antiserotonin effect nor was it mediated via adrenergic mechanisms.The results obtained in a double sensitization with two IgE antibodies suggest that AA-344 may not impair antigen-antibody combi nation but probably prevents the release of chemical mediators including histamine. This assumption was supported by observation that AA-344 inhibited a reduction in the skin histamine content caused by the 72-hr PCA, without effect on the compound 48/80-induced histamine reduction. AA-344 also partially inhibited the IgGa-mediated 3-hr PCA in rats. These results indicate that the inhibitory action of AA-344 on the immediate hypersensitivity reactions is due to prevention of the release of chemical mediators from the mast cells, by acting on some process in sequential events leading to the mediator release following antigen-antibody combination.

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“…Moreover, the anaphylactic release of histamine from the isolated rat mast cells was mediated not only by IgE but also by IgGa (28). These observations led to the proposal that both rat IgE and IgGa may utilize the same receptor on the mast cell or some common process leading to histamine release (26)(27)(28)(29). However, differences between IgE and IgGa in the period for sensitization and an affinity to the binding site have also been observed (28).…”

Section: Animalmentioning

confidence: 99%

“…These observations led to the proposal that both rat IgE and IgGa may utilize the same receptor on the mast cell or some common process leading to histamine release (26)(27)(28)(29). However, differences between IgE and IgGa in the period for sensitization and an affinity to the binding site have also been observed (28). In fact, a significant histamine release from mast cells was induced by a simultaneous addition of antigen and antiserum containing IgGa, while at least several hours were required for sensitization with IgE in producing a considerable histamine release.…”

Section: Animalmentioning

confidence: 99%

INHIBITORY ACTION OF 6-ETHYL-3-(lH-TETRAZOL-5-YL)CHROMONE (AA-344) ON IgE-, IgGa- OR CHEMICAL AGENT-INDUCED HISTAMINE RELEASE FROM ISOLATED RAT MAST CELLS

Saijo

Ashida

Kuriki

et al. 1979

Japanese Journal of Pharmacology

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Abstract-Theantiallergic action of 6-ethyl-3-(I H-tetrazol-5-yl)chromone (AA-344) was studied on isolated rat peritoneal mast cells. AA-344 clearly inhibited the IgE mediated release of histamine caused by various concentrations of antigen and the 50% inhibitory concentration was 0.1 uM. On the IgGa-mediated release of histamine, a peak inhibition of AA-344 was observed at 10 pM. MATERIALS AND METHODS Animal:Male Sprague-Dawley rats weighing 250-350 g (8-10 weeks old) were used.Antigens: Ascaris extract (asc) was prepared from Ascaris swum obtained from miniature swine according to the method of Tada and Okumura (4). Dinitrophenylated asc (DNP asc) was prepared by the method of Little and Eisen (5). Egg albumin (EA) of hen egg white was purified 3 times with sodium sulfate solution (6).

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IGE AND IGGA ANTIBODY-MEDIATED RELEASE OF HISTAMINE FROM RAT PERITONEAL CELLS

Cited by 78 publications

References 23 publications

THE INTERACTION OF IgE WITH RAT BASOPHILIC LEUKEMIA CELLS

THE INTERACTION OF IgE WITH RAT BASOPHILIC LEUKEMIA CELLS

Antiallergic action of 6-ethyl-3-(1h-tetrazol-5-yl) chromone (aa-344) on immediate hypersensitivity reaction in rats

INHIBITORY ACTION OF 6-ETHYL-3-(lH-TETRAZOL-5-YL)CHROMONE (AA-344) ON IgE-, IgGa- OR CHEMICAL AGENT-INDUCED HISTAMINE RELEASE FROM ISOLATED RAT MAST CELLS

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