<i>Schizosaccharomyces pombe</i> possesses an unusual and a conventional hexokinase: biochemical and molecular characterization of both hexokinases

Petit, Thomas; Blázquez, Miguel Á.; Gancedo, Carlos

doi:10.1016/0014-5793(95)01451-9

Cited by 32 publications

(47 citation statements)

References 24 publications

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“…The hxk2 gene, which is required for glucose metabolism (Petit et al, 1996), was analyzed in ird mutants and compared with the wild-type strain. The expression level of the hxk2 gene increased approximately 2-fold in the ird5 mutant (Figure 2) while it decreased in the ird13 and ird14 mutants compared to the wild-type strain.…”

Section: Ability To Escape Glucose Repression In Ird Mutantsmentioning

confidence: 99%

Effects of glucose sensing/signaling on oxidative stress response in glucose repression mutants of Schizosaccharomyces pombe

Palabıyık

Ghods

Uçar³

2013

Genet. Mol. Res.

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ABSTRACT. The resistant to glucose repression mutants of Schizosaccharomyces pombe (ird5, ird13, and ird14) have a high tolerance to oxidative stress induced by H 2 O 2 . In all ird mutants, the increased expression level of the fbp1 gene can be interpreted as a lack of glucose repression in these mutants. To investigate the mechanisms of the oxidative stress response in ird mutants, we analyzed the transcription of stress response-related genes, sod1, ctt1, atf1, pap1, and sty1, under stressed and non-stressed conditions. We then analyzed the phosphorylation state of the Sty1-MAP kinase in ird mutants. Our findings support the concept of an adaptive response to oxidative stress in these mutants. In addition, these results imply that either glucose signaling mechanisms leading to glucose repression and glucose utilization as an energy source are regulated apart from each other or, like Saccharomyces cerevisiae, S. pombe might have additional glucose detection systems.

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Section: Ability To Escape Glucose Repression In Ird Mutantsmentioning

confidence: 99%

Effects of glucose sensing/signaling on oxidative stress response in glucose repression mutants of Schizosaccharomyces pombe

Palabıyık

Ghods

Uçar³

2013

Genet. Mol. Res.

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“…The Y. lipolytica hexokinase needed was obtained from Sac. cerevisiae CJM291 (Petit et al, 1996), a strain lacking all glucose-phosphorylating enzymes carrying plasmid pDB20-YlcHXK1 (Petit & Gancedo, 1999). In some cases, T6P was assayed by HPLC as described by De Virgilio et al (1993).…”

Section: Fig 2 Aggregation Of C Albicans Catps2/catps2 Mutantsmentioning

confidence: 99%

Disruption in Candida albicans of the TPS2 gene encoding trehalose-6-phosphate phosphatase affects cell integrity and decreases infectivity The EMBL accession number for the sequence reported in this paper is AJ242990.

et al. 2002

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The gene CaTPS2 encoding trehalose-6-phosphate (T6P) phosphatase from Candida albicans has been cloned and disrupted in this organism. The Catps2/Catps2 mutant did not accumulate trehalose but accumulated high levels of T6P. Disruption of the two copies of the CaTPS2 gene did not abolish growth even at 42 SC, but decreased the growth rate. In the stationary phase, the Catps2/Catps2 mutant aggregated, more than 50 % of its cells became permeable to propidium iodide and a large amount of protein was found in the culture medium. Aggregation occurred only at pH values higher than 7 and was avoided by osmoprotectants ; it was never observed during the exponential phase of growth. The mutant formed colonies with a smooth border on Spider medium. Mice inoculated with 15i10 6 c.f.u. of wild-type cells died after 8 days, while 80 % of those inoculated with the same number of c.f.u. of the Catps2/Catps2 mutant survived for at least 1 month. Reintroduction of the wild-type CaTPS2 gene in the Catps2/Catps2 mutant abolished the phenotypes described. It is hypothesized that the accumulation of T6P interferes with the assembly of a normal cell wall.

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“…2B), essentially the same analyses as those described above were carried out. It was found that the previously identified hxk2+ gene (Petit et al, 1996) is located within the region crucial for suppression ability, which is located in plasmid pHXK2 (Fig. 2B).…”

Section: Resultsmentioning

confidence: 88%

“…sds23+ and hxk2 genes and phenotype of osmosensitivity The sds23+ gene was identified originally as a multicopy suppressor for mutations of S. pombe protein phosphatase 1 (PP1) and 205 cyclosome/anaphasepromoting complex (APC), both of which are implicated in the progression from metaphase to anaphase (i.e., onset of anaphase) in the mitotic cell cycle (Ishii et al, 1996). The hxk2+ gene was also identified recently as the gene encoding an isozyme of hexokinase (Petit et al, 1996). Until now, no linkage between the sds23+ and hxk2+ genes and the Sty1 MAPK cascade has been reported.…”

Section: Resultsmentioning

confidence: 99%

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Characterization of multicopy suppressor genes that complement a defect in the Wis1-Sty1 MAP kinase cascade involved in stress responses in Schizosaccharomyces pombe.

Yamada

Ohmiya

Yamamoto

et al. 1997

J. Gen. Appl. Microbiol.

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The Wis1-Sty1 mitogen-activated protein (MAP) kinase cascade is one of the major signaling systems involved in a wide range of stress responses in Schizosaccharomyces pombe. It is known that dwisl and dstyl mutants exhibit highly pleiotropic phenotypes, including a phenotype of temperature sensitivity for growth. In this study, we screened multicopy suppressor genes that allow both the dwisl and dstyl mutants to grow simultaneously at a non-permissive temperature, 37°C. Two such multicopy suppressors were cloned and characterized as sds23+ and hxk2+ genes. The former is known to specify a protein that functions as a multicopy suppressor for mutations of the PP1 protein phosphatase and the 20S cyclosomelanaphase-promoting complex (APC), and the latter encodes hexokinase 2. It was revealed that the multicopy sds23+ gene restored a defect in the mating efficiency caused by the dwisl and dstyl mutations, whereas the multicopy hxk2+ gene suppressed a phenotype of heat-shock sensitivity for growth of these mutant cells. These findings are discussed with special reference to the Wis1-Sty1 MAP kinase signaling pathway in S. pombe.

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Schizosaccharomyces pombe possesses an unusual and a conventional hexokinase: biochemical and molecular characterization of both hexokinases

Cited by 32 publications

References 24 publications

Effects of glucose sensing/signaling on oxidative stress response in glucose repression mutants of Schizosaccharomyces pombe

Effects of glucose sensing/signaling on oxidative stress response in glucose repression mutants of Schizosaccharomyces pombe

Disruption in Candida albicans of the TPS2 gene encoding trehalose-6-phosphate phosphatase affects cell integrity and decreases infectivity The EMBL accession number for the sequence reported in this paper is AJ242990.

Characterization of multicopy suppressor genes that complement a defect in the Wis1-Sty1 MAP kinase cascade involved in stress responses in Schizosaccharomyces pombe.

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