Salvia officinalisattenuates bleomycin-induced oxidative stress and lung fibrosis in rats

Cognitive Facilitation and Antioxidant Effects of an Essential Oil Mix on Scopolamine-Induced Amnesia in Rats: Molecular Modeling of In Vitro and In Vivo Approaches

“…Moreover, recently our group reported that the administration of R. officinalis to zebrafish abolished AChE alteration and brain oxidative stress induced by Sco [38]. S. officinalis has been observed to have anti-inflammatory and antioxidant effects on lipopolysaccharide (LPS)-induced inflammation in rats [39] and attenuated bleomycin-induced oxidative stress and lung fibrosis in rats [40]. In line with early results, we assume that the presence of the bioactive compounds within the MO may potentially lead to its increased antioxidant potential.…”

Cymbopogon winterianus Essential Oil Attenuates Bleomycin-Induced Pulmonary Fibrosis in a Murine Model

“…Lipid peroxidation was determined based on the detection of thiobarbituric acid (TBA) reactive products [ 17 ]. A reaction with TBA can detect small amounts of lipid peroxides, and more particularly, the free malondialdehyde (MDA) produced during the oxidative breakdown of lipids and polyunsaturated fatty acids.…”

“…Homogenates were centrifuged at 3000× g for 10 min at 4 °C, and the supernatant was stored at −80 °C for the determination of oxidative stress markers (TBARS and SH). Superoxide dismutase (SOD) activity was based on the inhibition of the auto-oxidation of epinephrine to adenochrome in the presence of SOD (pH 10.2) [ 17 ]. Briefly, 20 mL of epinephrine (5 mg/mL) was added to the assay mixture containing 10 mL of bovine catalase (0.4 U/mL) and 62.5 mM sodium carbonate–sodium bicarbonate buffer.…”

“…In the samples prepared as previously described, CAT activity was assessed using the method previously reported by Bahri et al (2020) [17] at 240 nm. Briefly, the reaction mixture included H 2 O 2 in 0.019 M, 0.05 M phosphate buffer (pH 7), and 0.03 mL of lung sample.…”

“…The multifactorial etiopathogenetic factors involving continuous oxidative stress, unresolved inflammation, and tissue scarring have made the management of the idiopathic PF so challenging that few therapeutics have succeeded in the clinic, and they have failed to improve patient survival [ 15 ]. As most therapeutic agents (corticosteroids, antifibrotic, and immunosuppressant drugs) have focused on one step at a time of idiopathic PF pathogenesis, the results tend to be unpromising [ 16 , 17 ]. Thus, therapeutic approaches based on traditional knowledge, such as plant-derived compounds, able to act in different steps of idiopathic PF pathogenesis, might be a successful strategy to treat the disease [ 18 ].…”