SalmonellaCholeraesuis outer membrane vesicles: Proteomics and immunogenicity

Abstract

Salmonella enterica serotype Choleraesuis (S. Choleraesuis), Gram-negative facultative intracellular pathogen is capable of inducing the cholera in pigs whose symptoms manifest as fever, depression, septicemia, arthritis, and diarrhea. Infections with S. Choleraesuis has resulted in great economic loss for the swine breeding operations. Bacterial outer membrane vesicles (OMVs) play an important role in pathogenicity and host-pathogen interaction. In this study, we purified OMVs released by S. Choleraesuis strain χ3545 and characterized their lipopolysaccharide (LPS) profile. The OMVs contained intact LPS molecules. By using LC-MS/MS, we identified 192 proteins in the OMVs. In addition, the subcellular location and biological functions of the vesicles was predicted. The proteins were mainly derived from outer membranes and cytoplasm. Several proteins were immunoreactive and associated with the secretion pathway. Some putative multi-drug resistance-associated proteins were also identified. Furthermore, immunization experiment via intranasal or intraperitoneal route in mice demonstrated that S. Choleraesuis OMVs could elicit strong humoral and mucosal immune responses. Although OMVs as vaccine did not provide strong protection against clinical strain of wild-type S. Choleraesuis, immunization of OMVs still prolonged the survival time of vaccinated mice after high dose of S. Choleraesuis infection. Overall, this study provides valuable fundamental information toward elucidating the pathogenicity and functions of OMVs secreted from S. Choleraesuis.

Salmonella enterica Serotype Choleraesuis var Decatur Gastroenteritis Complicated by Severe Acute Kidney Injury and Neurological Dysfunction

“…Salmonella enterica serotype Choleraesuis is a gram-negative host-adapted, facultative intracellular pathogen. 1,2 It is increasingly recognized as a major cause of systemic salmonellosis with a high mortality rate, is a major disease of the pigs, and causes various symptoms and complications like fever, depression, septicemia, arthritis, diarrhea, pneumonia, hepatitis, meningitis, and other chronic wasting diseases. 1,3,4 Serotype Choleraesuis differs from other salmonellae by the frequency with which it invades the bloodstream causing a generalized infection in humans.…”

Secretion and Delivery of Intestinal Pathogenic Escherichia coli Virulence Factors via Outer Membrane Vesicles

“…However, the molecular mechanism of virulence factor delivery via vesicles has been unclear. In addition to their production during infection, the key role of OMVs in bacterial virulence is supported by their ability to mimic in animal models diseases caused by the parental pathogens (Kim et al, 2011;Shah et al, 2012;Svennerholm et al, 2017), and to induce protective immune responses (Roy et al, 2011;Leitner et al, 2015;Roier et al, 2016;Liu et al, 2017).…”

Biofilm formation and extracellular microvesicles—The way of foodborne pathogens toward resistance

“…Cho et al [167] gave proteomic profile of outer membrane proteins in S. enterica Enteritidis. However, the proteome of isolated MVs was not additionally investigated, but Liu et al [168] gave a complete proteomic profile of MVs produced by this dangerous food pathogen. Interestingly, like in other models with Gram negative bacteria, one of the first signs of growth under stress conditions is downregulation of one of the key proteins, flagellin [38,39,168].…”

“…However, the proteome of isolated MVs was not additionally investigated, but Liu et al [168] gave a complete proteomic profile of MVs produced by this dangerous food pathogen. Interestingly, like in other models with Gram negative bacteria, one of the first signs of growth under stress conditions is downregulation of one of the key proteins, flagellin [38,39,168]. This work is one of the first steps in the development of MVs for vaccination against this dangerous foodborne pathogen that causes an estimated 22.2% of foodborne diseases in China [169], mostly after consuming of poultry and eggs [170].…”