<i>S</i>-Adenosylmethionine (SAM)-Accumulating Sake Yeast Suppresses Acute Alcohol-Induced Liver Injury in Mice

Izu, Hanae; Shobayashi, Megumi; Manabe, Yasuko; Goto, Kuniyasu; Iefuji, Haruyuki

doi:10.1271/bbb.60377

Cited by 26 publications

(6 citation statements)

References 26 publications

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“…In our study, the average amount of ingested ethanol was about 14 g/kg BW every day for each animal, which is an extremely high dosage among recent studies [21][22][23][24]. Chronic alcohol drinking might increase food intake but interrupt absorption of various nutrients [25].…”

Section: Discussionmentioning

confidence: 63%

Taurine supplementation improves the utilization of sulfur-containing amino acids in rats continually administrated alcohol

Yang

Chien

Tsen

et al. 2009

The Journal of Nutritional Biochemistry

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Section: Discussionmentioning

confidence: 63%

Taurine supplementation improves the utilization of sulfur-containing amino acids in rats continually administrated alcohol

Yang

Chien

Tsen

et al. 2009

The Journal of Nutritional Biochemistry

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“…Animal experiments are useful for examining the direct effects of pure alcohol. However, experimental animal models have focused on high toxicological doses with forced and excessive ingestion (e.g., intragastric ethanol infusion and liquid diets) (8,9). Meanwhile, animal studies involving low alcohol intake are limited.…”

Section: Introductionmentioning

confidence: 99%

Effects of low dose of ethanol on the senescence score, brain function and gene expression in senescence-accelerated mice 8 (SAMP8)

Kimoto

Izu

et al. 2017

Experimental and Therapeutic Medicine

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Accumulating epidemiological evidence suggests light to moderate alcohol intake reduces risk of several chronic diseases. However, there is limited information regarding the effects of low alcohol intake in animal studies. This study investigated the effect of low ethanol dosage on senescence-accelerated mouse (SAMP8), an animal model of aging and neurodegenaration. Male SAMP8 mice (11 weeks old) had free access to a commercial stock diet with drinking water containing 0, 1 or 2% (v/v) ethanol for 15 weeks. The total grading score of senescence in the 1%-ethanol group was, in large part, the lowest among the three groups. Analysis using the open-field test revealed a significant elevation (+77%, P<0.05) in the rearing activity (index of seeking behavior) in the 1%-ethanol group, but not in the 2%-ethanol group. In addition, 2% ethanol elevated spontaneous locomotor activity (+75%, P<0.05), whereas 1% ethanol did not. Scrutiny of serum parameters indicated intake of 1% ethanol significantly decreased serum insulin levels (−13%, P<0.05), whereas 2% did not. Intake of 2% ethanol significantly elevated (2.5-fold, P<0.05) S100a8 mRNA (an inflammatory signal) in the brain, but that of 1% ethanol did not. Intriguingly, 1% ethanol intake remarkably elevated (10-fold, P<0.05) mRNA of brain alcohol dehydrogenase 1 (Adh1), which metabolizes lipid-peroxidation products and is involved in the synthesis of retinoic acid, a neuroprotective factor. Of note, 2%-ethanol intake did not exert this effect. Taken together, intake of 1% ethanol is likely to be beneficial for SAMP8 mice.

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“…SAM has been proven as a chemotherapeutic reagent with various clinical effects. It is well acknowledged to lessen alcoholic liver injury [3]. Also, the daily intake of SAM has been shown to reduce the symptoms of osteoarthritis [4], depression [5], and Alzheimer disease [6].…”

Section: Introductionmentioning

confidence: 99%

Increased production of S-adenosyl-L-methionine using recombinant Saccharomyces cerevisiae sake K6

Choi

Park

Lee

et al. 2009

Korean J. Chem. Eng.

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S. cerevisiae sake K6 was the firstly isolated industrial strain to overproduce S-adenosyl-L-methionine (SAM). Although the strain has advantages over other strains, such as GRAS (generally recognized as safe) property, the S. cerevisiae K6 has not been further developed with DNA recombinant technology due to the lack of a proper genetic marker. In this study, UV mutagenesis was conducted with S. cerevisiae sake K6. With the method, a mutant of sake yeast with leucine auxotroph, K6-1, was isolated. The mutant showed comparable growth rate and SAM productivity with its wild type. Using the auxotroph as a genetic marker, a SAM synthase in S. cerevisiae, SAM2, was overexpressed in the mutant strain. This recombinant DNA technology successfully increased SAM productivity in sake yeast.

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S-Adenosylmethionine (SAM)-Accumulating Sake Yeast Suppresses Acute Alcohol-Induced Liver Injury in Mice

Cited by 26 publications

References 26 publications

Taurine supplementation improves the utilization of sulfur-containing amino acids in rats continually administrated alcohol

Taurine supplementation improves the utilization of sulfur-containing amino acids in rats continually administrated alcohol

Effects of low dose of ethanol on the senescence score, brain function and gene expression in senescence-accelerated mice 8 (SAMP8)

Increased production of S-adenosyl-L-methionine using recombinant Saccharomyces cerevisiae sake K6

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