S‐Adenosyl‐l‐Methionine Salvage Impacts Psilocybin Formation in “Magic” Mushrooms

Abstract

Figure 3. Characterization of P. cubensis SahH.Michaelis-Menten kinetics of SahH A) in hydrolytic direction (SAH as substrate),a nd synthetic direction with B) AdoorC )HCy as substrates.D)Impact of free NAD + on SahH activity.Catalytic activity in samples not supplemented with NAD + is probably due to carryover of enzyme-boundco-substrate from the heterologous protein production host.

Taking Different Roads: l‐Tryptophan as the Origin of Psilocybe Natural Products

“…Methanolic Psilocybe crude extracts (2 to 23 μg ml À 1 ) have shown a lethal effect on brine shrimp (Arthropoda), along with ambiguous effects on Caenorhabditis elegans (Nematoda). [30] These data, along with alkaloid production that is triggered in the fungus upon formation of the ephemeral fruiting bodies, [31] suggest that nematodes are not psilocybin's primary target. In addition, toxicity of psilocybin in mammals is near negligible in terms of acute physiological damage, with a LD 50 /ED 50 ratio > 1000 in mice.…”

Chemoenzymatic Synthesis of 5-Methylpsilocybin: A Tryptamine with Potential Psychedelic Activity

“…UHPLC/ESIMS was performed with 0.1% (v/v) FA in water (solvent A) and ACN (solvent B) under described conditions and settings . For LC/HRESIMS, ionization was in positive mode.…”

A draft reference assembly of the Psilocybe cubensis genome

“…This compound has been classified as a “breakthrough therapy” for depression by the FDA ( Johnson and Griffiths 2017 ). The psilocybin pathway was identified by Fricke et al, but to date no public references exist in NCBI with N50s longer than 50kb ( Fricke et al 2017 ; Blei et al 2018 ; Fricke et al 2019a ; Fricke et al 2019b ; Blei et al 2020 ; Demmler et al 2020 ; Fricke et al 2020 ). A more contiguous genome assembly can assist in further resolution of the genetic diversity in the fungi’s secondary metabolite production.…”