Rothia mucilaginosaPneumonia Diagnosed by Quantitative Cultures and Intracellular Organisms of Bronchoalveolar Lavage in a Lymphoma Patient

Abstract

Rothia mucilaginosa is a gram-positive coccus of the family Micrococcaceae. R. mucilaginosa is considered a part of the normal flora of the human oropharynx and upper respiratory tract and lower respiratory tract infections attributable to R. mucilaginosa are not frequent. We present a case of pneumonia, in which the R. mucilaginosa infection was diagnosed by quantitative cultures of a bronchoalveolar lavage (BAL) specimen. A 46-yr-old woman with B lymphoblastic lymphoma was admitted to the hospital for scheduled chemotherapy. Her chest computed tomography (CT) scan revealed bilateral multifocal nodular and patchy consolidation in both lungs. Investigation of the BAL specimen revealed that 7% of leukocytes had intracellular gram-positive cocci. The quantitative cultures of the BAL specimen grew mucoid, non-hemolytic, and grayish convex colonies on blood agar at a count of approximately 200,000 colony-forming units/mL. The colonies were identified as R. mucilaginosa. The patient was empirically treated with levofloxacin for 7 days, after which findings on the chest radiograph and CT scan improved. She was discharged with improvement on hospital day 46. To our knowledge, this is the first report of R. mucilaginosa pneumonia diagnosed in Korea. Quantitative culture of BAL specimen and examination of intracellular organisms are crucial for assessing the clinical significance of R. mucilaginosa recovered from the lower respiratory tract.

Cloacal Microbiome Structure in a Long-Distance Migratory Bird Assessed Using Deep 16sRNA Pyrosequencing

“…At a lower taxonomic level, many genera dominating in CM, such as Enterobacter , Streptococcus , Enterococcus , Clostridium , Lactobacillus , Lactococcus , Turicibacter and members of the Ruminococcaceae family, are facultative symbionts or commensals inhabiting the digestive tracts of many vertebrate species [ 2 , 56 , 62 ]. We also detected several OTUs, such as Staphylococcus , Janthinobacterium , Corynebacterium , Aerococcus and Brevibacterium , that commonly colonise the skin’s surface [ 63 ] and OTUs associated with other parts of the animal’s body, such as Rothia , Porphyromonas Enhydrobacter and Actinobacillus , see for example [ 64 ]. Finally, barn swallow CM composition may also partly reflect the bird’s diet, which is composed of flying insects and other arthropods present in aerial plankton.…”

Utility of 16S rDNA Sequencing for Identification of Rare Pathogenic Bacteria

“…yeei ) (Table ), which could not be identified using phenotypic identification systems. In the case of Rothia mucilaginosa, however, the API kits we employed were not suitable ; hence, an unknown identification was scored. The latter exemplified the additional cost that would be necessary to procure additional API kits for the identification of rare and uncommon bacteria species.…”

Bacteriemia causada por Rothia mucilaginosa después de neumonía en un paciente con trasplante de células progenitoras hematopoyéticas

“…La especie Rothia mucilaginosa forma parte de la flora normal de la orofaringe y las vías respiratorias altas, y no es un agente patogénico frecuente en los pacientes inmunocompetentes. 2,3 Esta bacteria es un patógeno oportunista, especialmente para los pacientes inmunodeprimidos, y puede causar bacteriemia, meningitis, neumonía, infecciones en los huesos y las articulaciones, infecciones cutáneas y en partes blandas, endocarditis, peritonitis e infecciones en prótesis. La neoplasia hematológica subyacente, la neutropenia grave, la presencia de una sonda permanente y el daño en las mucosas son los En general, esta bacteria es sensible a los glucopéptidos, las cefalosporinas de tercera generación, los fármacos carbapenémicos y la rifampicina.…”