<i>Rheum palmatum</i> root extract inhibits hepatocellular carcinoma in rats treated with diethylnitrosamine

El-Saied, Mahmoud A; Sobeh, Mansour; Abdo, Walied; Badr, Osama; Youssif, Lamiaa; Elsayed, Ibrahim

doi:10.1111/jphp.12899

Cited by 13 publications

(8 citation statements)

References 29 publications

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“…Oppositely, HCC group showed diffused white nodules on the liver with a pale appearance and liver photomicrograph stained with H&E showed loss of normal architecture of hepatocytes, neoplastic hepatocytes with pleomorphism, increased nuclear to cytoplasmic ratio (N/C), nuclear atypia and hyperchromasia (black arrows). Areas of hemorrhagic necrosis (H) invaded by mononuclear cellular infiltration (K; kupffer cells) are also observed (Figure 7), this was in accordance with previous studies (22,64,65). Figure 8 showed no gross lesions on the liver surface of HCC-bearing rats treated with CM-Ig and its photomicrograph stained with H&E showed diffuse vacuolization of hepatocytes.…”

Section: Cm-igg Treatment Alleviated Dena-induced Hepatic Histo-morphological Alterationssupporting

confidence: 89%

Camel (Camelus Dromedarius) Milk Antibodies Ameliorated Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats

et al. 2021

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Hepatocellular carcinoma (HCC) is an extremely aggressive solid tumor and relates to numerous cases of cancer-associated deaths globally. Current studies reveal that natural compounds retain various therapeutic effects. This study was aimed to investigate the beneficial antitumor effects of camel (Camelus dromedarius) milk antibodies (CM-IgG) on diethylnitrosamine (DENA)-induced hepatocellular carcinoma in male Wistar rats. Hepatocarcinoma was induced in rats using DENA (50 mg/kg, twice/week) for 2 weeks followed by CCl4 (1 ml/kg, trice/week) for 6 weeks. At week 17 th , HCC-bearing rats were orally administrated CM-IgG (100 mg/kg, orally) daily for 4 weeks. Liver enzyme activities and levels of alpha-fetoprotein (AFP) were measured in serum. Lipid peroxidation, nitric oxide, reduced glutathione (GSH) levels and superoxide dismutase (SOD) activity were determined in liver homogenate. Histological analysis using hematoxylin and eosin stain was examined in liver tissues. Hepatic mRNA gene expression of placental glutathione-s-transferase (GST-P) was determined by qRT-PCR. Treatment HCC-bearing rats with CM-IgG significantly reduced liver injury biomarkers and attenuated oxidative stress as well as enhanced antioxidant status. Moreover, CM-IgG significantly alleviated hepatocellular morphology alterations and down-regulated GST-P gene expression levels in liver. Our results revealed the potential role of total immunoglobulin IgG purified from camel milk in amelioration of DENA-induced hepatocellular dysfunction and oxidative stress in hepatocarcinoma-bearing rats.

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Section: Cm-igg Treatment Alleviated Dena-induced Hepatic Histo-morphological Alterationssupporting

confidence: 89%

Camel (Camelus Dromedarius) Milk Antibodies Ameliorated Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats

et al. 2021

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“…The majority of components in the screened fractions were identified as anthraquinones and polyphenols. Compounds 6, 7, 10, 12, 14, 16, 23, and 24 exhibited the neutral loss of CO and CO 2 , which is a characteristic feature of anthraquinones in the negative mode [18,[20][21][22]. Some reports have pointed out that anthraquinones possess inhibitory activity against EGFR [23].…”

Section: Resultsmentioning

confidence: 98%

“…ex Balf., and Rheum officinale Baill. The RRR extracts have been reported to exhibit a wide array of pharmacological properties including laxative, hepatoprotective, antiviral, and antitumor effects [19,20]. In this study, an HPLC peak fractionation approach combined with HTRF analysis was applied for screening EGFR inhibitors in the RRR extract.…”

Section: Introductionmentioning

confidence: 99%

Rapid Screening for EGFR Inhibitor in Rhei Radix et Rhizoma by HTRF Assay Coupled with HPLC Peak Fractionation

Xie

Zhao

et al. 2020

Planta Med

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In this paper, an HPLC peak fractionation approach combined with homogeneous time-resolved fluorescence analysis is proposed for screening epidermal growth factor receptor inhibitors from Rhei Radix et Rhizoma. With this approach, the amount of sample used for a single HPLC run is sufficient for performing a multiple assay due to the miniaturization ability of the homogeneous time-resolved fluorescence technology. This allows for improving the stability and repeatability of the activity assay for each fraction. From a total of 26 fractions collected from the Rhei Radix et Rhizoma extract, 13 fractions exhibit inhibitory activity against the epidermal growth factor receptor. The structures of activity compounds were determined by HPLC-LTQ-Orbitrap MS, revealing the presence of gallic acid, rhein, and emodin with IC50 values of 21.5, 5.29, and 10.2 µM, respectively. The ligand epidermal growth factor receptor interactions were explored by molecular docking simulations, and the inhibitory effects of the three compounds on A549 cell growth were tested in vitro by an MTT assay. This study demonstrates the suitability of the present screening method for drug discovery in natural products.

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“…We attempted to determine the appropriate decoction time for FDY2004 that would result in fewer side effects. Rheum palmatum, 34 Paeonia suffruticosa, 35 and Prunus davidiana, 36 the constituents of FDY2004, are known for their antiproliferative efficacy. Formulations containing combinations of these species may differ in efficacy and side effects depending on the extraction method used.…”

Section: Discussionmentioning

confidence: 99%

Influence of Decoction Duration of FDY2004 on Its Physicochemical Components and Antioxidant and Antiproliferative Activities

Lee¹,

Lee

Kang

et al. 2020

Natural Product Communications

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FDY2004 (Medicinal herbs: Rheum palmatum, Paeonia suffruticosa Andrews, and Prunus davidiana), an herbal drug composition with an antiproliferative effect, is prepared by boiling, which is the most common herbal extraction method in traditional Korean medicine. Several parameters are considered in the process, including herb-to-solvent ratio, extraction temperature and pressure, and total decoction time. The aim of this study was to examine the physicochemical changes, index compound analysis results, antioxidant activity, and antiproliferative activity of FDY2004 according to the decoction duration to establish the conditions that ensure efficacy while minimizing side effects. Different samples of FDY2004 were obtained by decocting for 30, 60, 90, 120, 180, and 240 minutes. Each sample was evaluated for hydrogen ion concentration (pH), total soluble solid content (TSSC), index compound profiles, and antioxidative and antiproliferative activity. pH was found to decrease, while TSSC increased with an increase in decoction duration. Index compound contents for FDY2004 (aloe emodin, emodin, rhein, chrysophanol, physcion, and sennoside A for R. palmatum, paeonol for P. suffruticosa Andrews, and amygdalin for P. davidiana) increased when the decoction duration was 120 minutes or more, while the content of sennoside A did not increase. The total d-glucose amount increased with an increase in boiling duration. Antioxidant activity of FDY2004 increased when the decoction duration was 120 minutes or more, and the antiproliferative activity of FDY2004 was concentration dependent. The decoction duration for FDY2004 needs to be carefully determined so as to maintain efficacy while reducing side effects related to digestive absorption.

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Rheum palmatum root extract inhibits hepatocellular carcinoma in rats treated with diethylnitrosamine

Abstract: Rheum palmatum is a potential candidate to be explored for the treatment of hepatocellular carcinoma.

Cited by 13 publications

References 29 publications

Camel (Camelus Dromedarius) Milk Antibodies Ameliorated Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats

Camel (Camelus Dromedarius) Milk Antibodies Ameliorated Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats

Rapid Screening for EGFR Inhibitor in Rhei Radix et Rhizoma by HTRF Assay Coupled with HPLC Peak Fractionation

Influence of Decoction Duration of FDY2004 on Its Physicochemical Components and Antioxidant and Antiproliferative Activities

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