RHCE‐D‐CE hybrid genes can cause false‐negative DNA typing of the Rh e antigen

Abstract

The presence of RHCE-D5-CE hybrid alleles may cause false-negative DNA-typing results for the Rh e antigen that are easily overlooked unless appropriate RH hybrid PCR-SSPs are incorporated into conventional DNA-typing protocols. These and previous data strongly caution against an uncritical interpretation of RH DNA-typing results.

RHCE alleles detected after weak and/or discrepant results in automated Rh blood grouping of blood donors in Northern Germany

“…Molecular typing can detect variants that are currently missed by serologic methods. However, even these approaches may miss some antigens of variant alleles, like the e antigen expressed by RHCE-D(5)-CE) 42 and RHce(P221R). The definition of the molecular bases of variant RHCE antigen expression remains important to optimize such genotyping approaches.…”