<i>RHC</i> and <i>RHc</i> genotyping in different ethnic groups

Tax, Martine G H M; Schoot, C. Ellen van der; Doorn, René van; Douglas-Berger, Lotte; Rhenen, D.J. van; Maaskant-vanWijk, Petra A

doi:10.1046/j.1537-2995.2002.00096.x

Cited by 44 publications

(46 citation statements)

References 32 publications

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“…The results obtained after analysis of sample 1 confirmed that it is not possible to obtain accurate RhC/c results by testing the RHCE nucleotide 48 polymorphism alone. This polymorphism does not correlate with the RhC/c phenotype, particularly in the Dce haplotype [10,11].…”

Section: Methods and Resultsmentioning

confidence: 99%

Report of the First International Workshop on molecular blood group genotyping

2005

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The use of molecular genetic technology for blood group typing is becoming routine procedure in many reference laboratories worldwide. A First International Workshop was organized on behalf of the International Society of Blood Transfusion (ISBT) and the International Council for Standardization in Haematology (ICSH). Thirty laboratories that provide a molecular diagnostic service participated in the workshop. Six samples were distributed: two represented DNA from transfusion-dependent patients for testing for multiple polymorphisms; two represented fetal DNA prepared from amniotic fluid for RhD, Rhc and K-testing; and two represented plasma from RhD-negative pregnant women for fetal RhD testing. Error rates varied from 0 to 11% for different polymorphisms. A consensus arising from discussion on the workshop results between participants at a feedback meeting and by e-mail has resulted in seven recommendations for molecular blood group genotyping. Further international workshops will take place every 2 years, with a more limited exercise being organized in the intervening years.

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Section: Methods and Resultsmentioning

confidence: 99%

Report of the First International Workshop on molecular blood group genotyping

2005

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“…However, because different ethnic groups have different genetic background and especially numerous variant RHCE alleles, many DNA genotyping techniques were found not reliable. Tax et al [23] mentioned that RHCE*C genotyping based on 48C was not reliable because of a 48G>C mutation in the RHCE*ce allele (false-positive prediction of C), and genotyping on intron 2 is unreliable because of r's (Cdes) alleles (false-negative prediction of C). Then, in France, Pham et al [24] and Kappler-Gratias et al [25] reported 572/806 individuals and 14.2% African blood donors, respectively, with RhCE variants or variant RHCE alleles.…”

Section: Discussionmentioning

confidence: 99%

Rh-Matched Transfusion through Molecular Typing for β-Thalassemia Patients Is Required and Feasible in Chinese

Shao

Zhao

et al. 2018

Transfus Med Hemother

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Background: Molecular typing for RHCE blood group alleles has been established in many countries for patients and blood donors. In the Chinese literature nearly 80% of transfused patients with alloimmunization have antibodies specific for antigens of the Rh blood group system. We investigated if it is feasible to match packed red blood cells (RBCs) for Chinese β-thalassemia patients by RHCE genotyping. Methods: In this study, 481 patients with β-thalassemia were enrolled. They were genotyped for RHCE alleles by a simple PCR method with sequence-specific primers (PCR-SSP). Among these patients, 203 continuously received RBCs of the identical Rh subgroups according to the genotyping results for at least 3 months. Subsequently, their phenotypes were tested through a micro-column gel card method. For validation purposes, 400 donors were serologically typed with the same technology, of which 164 were genotyped too. Finally, the C, c, E, and e frequencies and the feasibility of the simple genotyping method were analyzed. Results: All patients showed mixed-field agglutination in the Rh subgroup gel cards before the same Rh subgroups in blood donors were selected for blood transfusion. The results, however, lacked mixed-field agglutination in all 203 cases after transfusion with RBC concentrates selected for the patient's C, c, E, and e antigens for at least 3 months. The genotyping results of 164 donors were all consistent with the serological results. Whole coding regions of RHCE were sequenced in 7 individuals with weak c, E, or e antigens. In only one sample we observed a 1059G>A nucleotide mutation coding for a truncated RhCE polypeptide (GenBank KT957625), in the other 6 samples no sequence variant was found. Both patients and donors were predominantly CcEe and CCee, with a prevalence of 55.3% and 24.9% for patients or 49.3% and 31.3% for donors, respectively. It revealed that about 80% of Chinese could receive Rh-matched RBCs easily. Conclusion: A simple RHCE genotyping technique is safe enough for Rh-matched transfusion of β-thalassemia patients in Chinese Han.

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“…Exons 2 of RHD and RHCE * C have an identical sequence, so it is not possible to design allele -specifi c primers for identification of RHCE * C (except in D − samples), though it is relatively straightforward to design them for RHCE * c [128,318,321] . Exons 2 of RHD and RHCE * C have an identical sequence, so it is not possible to design allele -specifi c primers for identification of RHCE * C (except in D − samples), though it is relatively straightforward to design them for RHCE * c [128,318,321] .…”

Section: Predicting C/c Phenotype From Dnamentioning

confidence: 99%