Retracted: Zerumbone inhibits epithelial‐mesenchymal transition and cancer stem cells properties by inhibiting the β‐catenin pathway through miR‐200c

Abstract

Colorectal cancer (CRC) is one of the most lethal and rampant human malignancies in the world. Zerumbone, a sesquiterpene isolated from subtropical ginger, has been found to exhibit an antitumor effect in various cancer types. However, the effect of Zerumbone on the biological properties of CRC, including epithelial-mesenchymal transition (EMT) and cancer stem cells (CSCs) has not been fully elucidated. Here, we investigated the inhibitory action of Zerumbone on the EMT process, CSC markers, and the β-catenin signaling pathway in the presence or absence of miR-200c. The effect of Zerumbone on HCT-116 and SW-48 cells viability was examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effects of Zerumbone on EMT-related genes, CSCs markers, cell migration, invasion, sphere-forming, and β-catenin signaling pathway were explored. To evaluate the role of miR-200c in anticancer effects by Zerumbone, miR-200c was downregulated by LNA-anti-miR-200c. Zerumbone significantly inhibited cell viability, migration, invasion, and sphere-forming potential in HCT-116 and SW-48 cell lines. Zerumbone significantly suppressed the EMT and CSC properties as well as downregulated the β-catenin. Silencing of miR200c reduced the inhibitory effects of Zerumbone on EMT and CSCs in CRC cells. These data indicated that Zerumbone may be a promising candidate for reducing the risk of CRC progression by suppressing the β-catenin pathway via miR-200c.

Role of Wnt/β-Catenin Signaling in the Chemoresistance Modulation of Colorectal Cancer

“…Liu et al [51] showed that CD146 decreased the drug resistance of colorectal cancer by functioning as a suppressor of cancer stemness through inactivating the Wnt/β-catenin cascade. It was also observed that zerumbone could suppress the stemness properties of CRC by inhibiting the β-catenin signaling pathway [52]. These studies all suggested that the Wnt/β-catenin signaling pathway plays crucial roles in fostering chemoresistance of CRC through stemness.…”

Protective Effects of Zerumbone on Colonic Tumorigenesis in Enterotoxigenic Bacteroides fragilis (ETBF)-Colonized AOM/DSS BALB/c Mice

“…A recent study by Dermani et al showed that zerumbone treatment of HCT116 and SW48 colorectal cell lines inhibited cell viability, migration, invasion and sphere-forming potential via miR-200c [32]. Silencing of miR-200c reduced the anti-cancer effects of zerumbone.…”

Small molecule inhibitors of epithelial‐mesenchymal transition for the treatment of cancer and fibrosis

“…Recently, it was reported that zerumbone inhibited the β-catenin pathway via miR-200c to block EMT and cancer stem cells. 26 In addition, nicotine upregulated FGFR3 and RB1 and promoted EMT by the downregulation of miR-99b and miR-192 in non-small-cell lung cancer cells. 50 Moreover, osthole alleviated EMT-mediated metastasis by inhibiting miR-23a-3p.…”

“…Zerumbone from Zingiber zerumbet (L.) Smith exhibited anti‐inflammatory and anticancer properties. Recently, it was reported that zerumbone inhibited the β‐catenin pathway via miR‐200c to block EMT and cancer stem cells . In addition, nicotine upregulated FGFR3 and RB1 and promoted EMT by the downregulation of miR‐99b and miR‐192 in non–small‐cell lung cancer cells .…”