<i>Retracted</i>: Silencing of keratin 1 inactivates the Notch signaling pathway to inhibit renal interstitial fibrosis and glomerular sclerosis in uremia

Chen, Wen; Wang, Zhikui; Zhang, Lei; Sun, Lina; Man, Yan-Gao; Zhang, Zhou

doi:10.1002/jcp.29087

Cited by 7 publications

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some scholars found that the expressions of Notch1 and Jagged1 were significantly upregulated in the kidney tissues of IgA nephropathy, and Notch1, HEY1, and HES1 were significantly reduced after administration of the Notch1 pathway inhibitor DAPT, thereby alleviating RIF [ 49 ]. Notch pathway inhibitors were found to weaken glomerulosclerosis and RIF by decreasing the expression of Jagged1, Notch1, NICD1, HEY1, HES1 α -SMA, and fibronectin in uremic rats [ 50 ]. Thus, inhibition of the Notch pathway can alleviate RIF to a certain extent.…”

Section: Discussionmentioning

confidence: 99%

Shen Qi Wan‐Containing Serum Alleviates Renal Interstitial Fibrosis via Restraining Notch1‐Mediated Epithelial‐Mesenchymal Transition

Chen

Zhou

Yang

et al. 2023

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Objective. Shen Qi Wan (SQW) is the most classic prescription for the clinical therapy of chronic kidney disease in China. Nevertheless, the function of SQW in renal interstitial fibrosis (RIF) has not been clearly clarified. Our purpose was to explore the protective function of SQW on RIF. Methods. After intervention with SQW-containing serum alone at increasing concentrations (2.5, 5, and 10%) or in combination with siNotch1, the transforming growth factor-beta (TGF-β)-induced HK-2 cell viability, extracellular matrix (ECM)-, epithelial-mesenchymal transition (EMT), and Notch1 pathway-associated protein expressions were assessed by cell counting kit-8, qRT-PCR, western blot, and immunofluorescence assays. Results.SQW-containing serum intensified the viability of TGF-β-mediated HK-2 cells. Besides, it augmented the collagen II and E-cadherin levels, and weakened the fibronectin, α-SMA, vimentin, N-cadherin, and collagen I levels in HK-2 cells triggered by TGF-β. Moreover, it is found that TGF-β led to the upregulation of Notch1, Jag1, HEY1, HES1, and TGF-β in HK-2 cells, which was partially offset by SQW-containing serum. Furthermore, cotreatment of SQW-containing serum and Notch1 knockdown further apparently alleviated the Notch1, vimentin, N-cadherin, collagen I, and fibronectin levels in HK-2 cells induced by TGF-β. Conclusion. Collectively, these findings elucidated that SQW-containing serum attenuated RIF via restraining EMT through the repression of the Notch1 pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Shen Qi Wan‐Containing Serum Alleviates Renal Interstitial Fibrosis via Restraining Notch1‐Mediated Epithelial‐Mesenchymal Transition

Chen

Zhou

Yang

et al. 2023

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…The observation of KRT10 expression in every tissue within the GTEx database is in agreement with numerous prior reports of expression in skin [ 55 ], breast [ 56 ], testis [ 57 ], cervix [ 58 ], thymus [ 59 ] and vagina [ 60 ]; and with the finding that expression of a transgene driven by the KRT10 promoter was observed in stomach, small intestine, cecum, colon, spleen, and pancreas [ 61 ]. While KRT1 expression is well established in skin integrity [ 55 , 62 ], colonic mucosa [ 63 ], kidney [ 64 ] and vagina [ 65 ], the GTEx data indicate that KRT1 has a much more expansive expression pattern than is suggested by the literature. These expression data also raise the question as to whether KRT10 is expressed in terminally-differentiated epithelial cells [ 66 ].…”

Section: Main Textmentioning

confidence: 99%

Update of the keratin gene family: evolution, tissue-specific expression patterns, and relevance to clinical disorders

Thompson²,

Fisk

et al. 2022

Hum Genomics

View full text Add to dashboard Cite

Intermediate filament (IntFil) genes arose during early metazoan evolution, to provide mechanical support for plasma membranes contacting/interacting with other cells and the extracellular matrix. Keratin genes comprise the largest subset of IntFil genes. Whereas the first keratin gene appeared in sponge, and three genes in arthropods, more rapid increases in keratin genes occurred in lungfish and amphibian genomes, concomitant with land animal-sea animal divergence (~ 440 to 410 million years ago). Human, mouse and zebrafish genomes contain 18, 17 and 24 non-keratin IntFil genes, respectively. Human has 27 of 28 type I “acidic” keratin genes clustered at chromosome (Chr) 17q21.2, and all 26 type II “basic” keratin genes clustered at Chr 12q13.13. Mouse has 27 of 28 type I keratin genes clustered on Chr 11, and all 26 type II clustered on Chr 15. Zebrafish has 18 type I keratin genes scattered on five chromosomes, and 3 type II keratin genes on two chromosomes. Types I and II keratin clusters—reflecting evolutionary blooms of keratin genes along one chromosomal segment—are found in all land animal genomes examined, but not fishes; such rapid gene expansions likely reflect sudden requirements for many novel paralogous proteins having divergent functions to enhance species survival following sea-to-land transition. Using data from the Genotype-Tissue Expression (GTEx) project, tissue-specific keratin expression throughout the human body was reconstructed. Clustering of gene expression patterns revealed similarities in tissue-specific expression patterns for previously described “keratin pairs” (i.e., KRT1/KRT10, KRT8/KRT18, KRT5/KRT14, KRT6/KRT16 and KRT6/KRT17 proteins). The ClinVar database currently lists 26 human disease-causing variants within the various domains of keratin proteins.

show abstract

Clinical utility of keratin 14 expression measurement in reflecting the tumor properties and prognosis in patients with renal cell carcinoma: a study with long-term follow-up

Wang,

Gao,

Wang

et al. 2024

Int Urol Nephrol

View full text Add to dashboard Cite

Retracted: Silencing of keratin 1 inactivates the Notch signaling pathway to inhibit renal interstitial fibrosis and glomerular sclerosis in uremia

Cited by 7 publications

References 39 publications

Shen Qi Wan‐Containing Serum Alleviates Renal Interstitial Fibrosis via Restraining Notch1‐Mediated Epithelial‐Mesenchymal Transition

Shen Qi Wan‐Containing Serum Alleviates Renal Interstitial Fibrosis via Restraining Notch1‐Mediated Epithelial‐Mesenchymal Transition

Update of the keratin gene family: evolution, tissue-specific expression patterns, and relevance to clinical disorders

Clinical utility of keratin 14 expression measurement in reflecting the tumor properties and prognosis in patients with renal cell carcinoma: a study with long-term follow-up

Contact Info

Product

Resources

About