Retracted: microRNA‐29b inhibits cell growth and promotes sensitivity to oxaliplatin in colon cancer by targeting FOLR1

Abstract

The present study was aimed to explore the functional role of microRNA (miR)-29b in colon cancer, as well as underlying mechanisms. Expressions of miR-29b and folate receptor 1 (FOLR1) were measured in both human colon tumor samples and cell lines.Colon cancer cell lines SW480 and SW620 were transfected with miR-29b mimic, antisense oligonucleotides (ASO)-miR-29b, small interfering (siRNA) against FOLR1 (si-FOLR1), or corresponding negative controls (NCs), and then were incubated with or without oxaliplatin (L-OHP). Thereafter, cell viability, cytotoxicity, cell apoptosis, and expression of FOLR1, ATP Binding Cassette Subfamily G Member 2 (ABCG2) and p-glycoprotein (p-gp) were analyzed. We found that miR-29b was significantly decreased, while FOLR1 was statistically elevated in colon cancer samples and cell lines compared to the nontumor samples and nontumourigenic immortalized human colon epithelial cell line FHC. Overexpression of miR-29b markedly inhibited cell viability, promoted sensitivity to L-OHP, stimulated cell apoptosis (all p < .05), and decreased the levels of ABCG2 and p-gp in cancer cells, whereas suppression of miR-29b showed contrary results. Moreover, we observed that FOLR1 was a direct target of miR-29b and was negatively regulated by miR-29b. In addition, the findings revealed that the effects of FOLR1 inhibition on cell viability, sensitivity to L-OHP, cell apoptosis, and the levels of ABCG2 and p-gp were similar to overexpression of miR-29b. Taken together, our study suggests that miR-29b inhibits cell growth and promotes sensitivity to L-OHP in colon cancer by targeting FOLR1. Highlights 1. miR-29b is decreased, while FOLR1 is elevated in cancer samples and cell lines; 2. miR-29b inhibits cell growth and promotes sensitivity to L-OHP in cancer cells;

Expression profile of microRNA-29b and its action on matrix metalloproteinase 2 (MMP-2) in the cervical carcinogenic process

“…The decrease in microRNA-29b expression has also been reported in other types of tumors (Sur et al 2019, He et al 2020, Fu et al 2020, however, only the study by Li et al 2017, had the expression of this microRNA in cervical samples was evaluated. Despite not considering all histopathological levels of cervical carcinogenic progression, they observed that the expression of microRNA-29b was suppressed when compared to normal tissues, corroborating our results.…”

Flaming the fight against cancer cells: the role of microRNA-93

“…MiR-132 is capable of significant reduction in the viability and proliferation of renal carcinoma cells [35]. These studies highlight this fact that identification of miRs and their subsequent targeting is a promising strategy in treatment of cancer as a life-threatening condition [36].…”

High Expression of Folate Receptor Alpha (FOLR1) is Associated With Aggressive Tumor Behavior, Poor Response to Chemoradiotherapy, and Worse Survival in Rectal Cancer

“… 30 , 31 Fu et al also demonstrated that FOLR1 expression promotes chemotherapy resistance. 32 Downregulation of FOLR1 significantly inhibited cell viability, enhanced sensitivity to oxaliplatin, and promoted cell apoptosis. The mechanism of chemotherapy resistance through stabilizing MDM2 in cooperation with chaperone protein prohibitin 2 was noted in FOLR1 expressing gastric cancer cells.…”