<i>Retracted:</i> Long non‐coding RNA XIST binding to let‐7c‐5p contributes to rheumatoid arthritis through its effects on proliferation and differentiation of osteoblasts via regulation of STAT3

Wang, Zongqiang; Xiu, Dianhui; Jiang, Jinlan; Liu, Gui‐Feng

doi:10.1002/jcla.23496

Cited by 16 publications

(11 citation statements)

References 57 publications

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“…Studies have clarified that lncRNA NEAT1 could bind to miR-129/miR-204 (188), miR-410-3p (189), and miR-23a (190), thereby regulating cell viability, migration, and inflammation in fibroblast−like synoviocytes from RA. Furthermore, the up-regulated lncRNA ZFAS1 (155,191), HIX003209 (156), and XIST (192) and down-regulated lncRNA LINC01197 (193), GAS5 (154), and OIP5-AS1 (194) also sponged miRNAs, thereby participating in cell proliferation, differentiation, apoptosis, and inflammation in synovial tissue of RA via ZFAS1/miR-2682-5p/ADAMTS9 axis, ZFAS1/miR-296-5p/MMP-15 axis, HIX003209/miR-6089/TLR4 axis, lncRNA XIST/let-7c-5p/STAT3 axis, LINC01197/miR-150/THBS2 axis, GAS5/miR-128-3p/HDAC4 axis, and OIP5-AS1/miR-448/ PON1, respectively. The details are presented in Table S1.…”

Section: The Crosstalk Of Lncrnas Mirnas and Mrnas In Ramentioning

confidence: 99%

Functional Interactions Between lncRNAs/circRNAs and miRNAs: Insights Into Rheumatoid Arthritis

2022

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Rheumatoid arthritis (RA) is one of the most common autoimmune diseases that affect synovitis, bone, cartilage, and joint. RA leads to bone and cartilage damage and extra-articular disorders. However, the pathogenesis of RA is still unclear, and the lack of effective early diagnosis and treatment causes severe disability, and ultimately, early death. Accumulating evidence revealed that the regulatory network that includes long non-coding RNAs (lncRNAs)/circular RNAs (circRNAs), micro RNAs (miRNAs), and messenger RNAs (mRNA) plays important roles in regulating the pathological and physiological processes in RA. lncRNAs/circRNAs act as the miRNA sponge and competitively bind to miRNA to regulate the expression mRNA in synovial tissue, FLS, and PBMC, participate in the regulation of proliferation, apoptosis, invasion, and inflammatory response. Thereby providing new strategies for its diagnosis and treatment. In this review, we comprehensively summarized the regulatory mechanisms of lncRNA/circRNA-miRNA-mRNA network and the potential roles of non-coding RNAs as biomarkers and therapeutic targets for the diagnosis and treatment of RA.

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Section: The Crosstalk Of Lncrnas Mirnas and Mrnas In Ramentioning

confidence: 99%

Functional Interactions Between lncRNAs/circRNAs and miRNAs: Insights Into Rheumatoid Arthritis

2022

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“…The lncRNA XIST affects the proliferation and differentiation of osteoblasts by binding let-7c-5p to regulate signal transducer and activator of transcription 3 ( STAT3 ), thereby promoting the occurrence of RA. 60 Quercetin can inhibit TNF-α-induced inflammatory cytokine production and XIST expression in RA-FLS, and silencing XIST can inhibit the inflammatory response of TNF-α-treated cells through miR-485. 4 The interaction between lncRNA XIST and miR-126-3p regulates the NF-κB signaling pathway in RA-FLS; downregulation of XIST can inhibit the proliferation of FLS by increasing the expression of miR-126-3p/NF-κB, thereby inhibiting the occurrence and development of RA.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis Identified the Hub Genes, mRNA–miRNA–lncRNA Axis, and Signaling Pathways Involved in Rheumatoid Arthritis Pathogenesis

Yang¹,

Su²,

Xu³

et al. 2022

IJGM

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Objective Rheumatoid arthritis (RA) is a nonspecific, chronic, systemic autoimmune disease characterized by symmetric polyarticular synovitis. Bioinformatics analysis of potential biomarkers, mRNA–miRNA–lncRNA axes, and signaling pathways in the pathogenesis of RA provides potential targets and theoretical basis for further research on RA. Methods The GSE1919 and GSE77298 datasets were downloaded from the Gene Expression Omnibus database ( http://www.ncbi.nlm.nih.gov/geo ). Perl was used to perform data merging, and R was used to perform batch correction. The “limma” package of R was used to screen differentially expressed genes, and the “clusterProfiler” package was used to perform enrichment analysis of the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. Search Tool for the Retrieval of Interacting Genes/Proteins was used to construct the protein–protein interaction network, Cytoscape was used for module analysis, and R was used to screen for hub genes. GraphPad Prism was used to plot the receiver operating characteristic curve of the hub genes. Gene set enrichment analysis and competitive endogenous RNA network analysis were performed on hub genes with the greatest diagnostic values. The hub gene with the greatest diagnostic value was verified using immunohistochemical staining. Results We obtained nine hub genes ( ITGB2, VAMP8, HLA-A, PTAFR, SYK, FCER1G, HLA-DPB1, LCP2 , and ACTR2 ) and four mRNA–miRNA–lncRNA axes (ITGB2-hsa-miR-486-3p-SNHG3, ITGB2-hsa-miR-338-5p-XIST, ITGB2-hsa-miR-5581-3p-XIST, and ITGB2-hsa-miR-1226-5p-XIST) related to the pathogenesis of RA. The nine hub genes were highly expressed, and ITGB2 had the highest diagnostic value for RA. We also identified signaling pathways related to the pathogenesis of RA: Fc epsilon Rl and chemokine signaling pathways. The immunohistochemical results showed that ITGB2 expression was significantly upregulated in RA. Conclusion The hub genes, mRNA–miRNA–lncRNA axes, and signaling pathways related to RA pathogenesis identified in this study provide a new research direction for the mechanism, diagnosis, and treatment of RA.

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“…[37][38][39] In patients with rheumatoid arthritis, high expression level of let-7c led to decreased levels of TNF-α, IL-2, and IL-6, suggesting suppression of the inflammatory response. 40 Let-7c can also regulate inflammation under oxidative stress in lens epithelial cells by targeting ATG3, which prevents the formation of cataracts. 41 In the present study, we found that let-7c was a key signaling factor for macrophage polarization and inhibition of UFB activation.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Exosomes Induced by IL-1β Attenuate Urethral Stricture Through Let-7c/PAK1/NF-κB-Regulated Macrophage M2 Polarization

Chen¹,

Dong²,

Hou³

et al. 2021

JIR

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Background: Urethral stricture is a clinical challenge for both patients and clinicians. Posttraumatic urethral stricture is associated with formation of scar tissue caused by excessive inflammation. The aim of this study is exploring potential therapeutic strategies for this condition. Methods: In vivo experiments on New Zealand rabbits and in vitro experiments on THP-1 monocytes and urethral fibroblasts were performed to investigate the effects on posttraumatic urethral stricture of exosomes isolated from IL-1β-treated mesenchymal stem cells (Exo-MSCs IL−1β ) and the role of macrophage M2 polarization in this process. Additionally, related signaling and mechanism behind were explored. Results: In a New Zealand rabbit model of post-traumatic urethral stricture, injection of Exo-MSCs IL−1β significantly reduced urethral stricture and collagen fiber accumulation compared with Exo-MSCs. Addition of Exo-MSCs IL−1β to THP-1 monocytes in vitro induced M2 macrophage polarization, which, in turn, inhibited activation of urethral fibroblasts and synthesis of collagen. Mechanistically, Exo-MSCs IL−1β were found to contain high levels of the microRNA let-7c, and luciferase reporter assays showed that let-7c interacted with the 3′UTR of PAK1 mRNA. Transfection of THP-1 cells with a let-7c mimic downregulated PAK1 expression and inhibited activation of the NF-κB signaling pathway. Conclusion: These results support a role for let-7c-containing Exo-MSCs IL−1β in reducing urethral stricture via inhibition of PAK1-NF-κB signaling, M2 macrophage polarization, and differentiation of urethral myofibroblasts.

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Retracted: Long non‐coding RNA XIST binding to let‐7c‐5p contributes to rheumatoid arthritis through its effects on proliferation and differentiation of osteoblasts via regulation of STAT3

Cited by 16 publications

References 57 publications

Functional Interactions Between lncRNAs/circRNAs and miRNAs: Insights Into Rheumatoid Arthritis

Functional Interactions Between lncRNAs/circRNAs and miRNAs: Insights Into Rheumatoid Arthritis

Bioinformatics Analysis Identified the Hub Genes, mRNA–miRNA–lncRNA Axis, and Signaling Pathways Involved in Rheumatoid Arthritis Pathogenesis

Mesenchymal Stem Cell-Derived Exosomes Induced by IL-1β Attenuate Urethral Stricture Through Let-7c/PAK1/NF-κB-Regulated Macrophage M2 Polarization

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