Retracted: Long non‐coding RNA PGM5‐AS1 promotes epithelial‐mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR‐140‐5p‐mediated FBN1 inhibition

Abstract

Osteosarcoma is an uncommon tumor occurring in bone, accompanied by elevated incidence and reduced rate of healing. Epithelial-to-mesenchymal transition (EMT) serves as a conceptual paradigm to explain the invasion and metastasis of osteosarcoma and other cancers. Hence, developing effective therapeutic strategy to treat the EMT of osteosarcoma is essential. Here, we identified the molecular mechanism of long noncoding RNA (lncRNA) PGM5-AS1 in EMT and progression of osteosarcoma. Microarray-based analysis was employed to screen the osteosarcoma-related differentially expressed lncRNAs. The levels of PGM5-AS1 as well as microRNA-140-5p (miR-140-5p) and fibrillin-1 (FBN1) in osteosarcoma tissues and cells were determined. Dual-luciferase reporter gene assay, RNA pull-down assay, and RNA immunoprecipitation assay were conducted to validate the relationship among PGM5-AS1, miR-140-5p, and FBN1. Expression of PGM5-AS1, miR-140-5p, and FBN1 was altered by overexpression, shRNA, mimic, or inhibitors in order to investigate how they regulated migration, invasion, and EMT of osteosarcoma cells in vitro. Loss-and gain-of-function approaches were employed in nude mice to detect their roles in tumorigenesis in vivo. Osteosarcoma tissues and cells exhibited low expression of miR-140-5p, but high expression of PGM5-AS1 and FBN1. PGM5-AS1 competitively bound to miR-140-5p to upregulate FBN1. Furthermore, hindering PGM5-AS1 and FBN1 or overexpressing miR-140-5p dampened migration, invasion, and EMT of osteosarcoma cells in vitro. Furthermore, silencing PGM5-AS1 or FBN1, or overexpressing miR-140-5p markedly inhibited tumorigenesis in nude mice in vivo. Taken together, PGM5-AS1 depletion causes FBN1 reduction to retard osteosarcoma processes by negatively modulating miR-140-5p.

Long non-coding RNA KCNQ1 overlapping transcript 1 promotes the progression of esophageal squamous cell carcinoma by adsorbing microRNA-133b

“…Long non-coding RNAs (lncRNAs) are evolutionarily conserved RNA molecules longer than 200 bases that have no or limited protein-coding functions ( 6 , 7 ). Recent studies have shown that lncRNAs play an important role in the tumorigenesis and development of various cancers ( 8 , 9 ). LncRNAs are frequently dysregulated in EC tissues and participate in cancer progression ( 10 , 11 ).…”

lncRNA TUSC7 inhibits osteosarcoma progression through the miR‑181a/RASSF6 axis

“…lncRNAs act as carcinogenic factors or tumor suppressor factors by regulating different mRNAs by sponging them ( 32 , 33 ). Several lncRNAs have been reported to be involved in OS progression ( 18 , 34 , 35 ). For example, as oncogenes, lncRNA PGM5-AS1 promotes epithelial-mesenchymal transition, invasion and metastasis of OS cells by impairing miR-140-5p-mediated fibrillin-1 inhibition ( 34 ).…”

“…Several lncRNAs have been reported to be involved in OS progression ( 18 , 34 , 35 ). For example, as oncogenes, lncRNA PGM5-AS1 promotes epithelial-mesenchymal transition, invasion and metastasis of OS cells by impairing miR-140-5p-mediated fibrillin-1 inhibition ( 34 ). On the other hand, lncRNAs can act as tumor suppressors.…”

Long noncoding RNA plasmacytoma variant translocation gene 1 promotes epithelial‐mesenchymal transition in osteosarcoma

“…The expression of lncRNA PGM5‐AS1 is upregulated in osteosarcoma. It regulates the expression of miR‐140‐5p and FBN1 through sponge adsorption, thereby promoting tumor EMT, invasion, and metastasis 13 . LncRNA CCAT2, a marker of poor prognosis in patients with osteosarcoma, was found to promote EMT in osteosarcoma cells 14 .…”

“…It regulates the expression of miR-140-5p and FBN1 through sponge adsorption, thereby promoting tumor EMT, invasion, and metastasis. 13 LncRNA CCAT2, a marker of poor prognosis in patients with osteosarcoma, was found to promote EMT in osteosarcoma cells. 14 Therefore, we also studied the relationship between lncRNA PVT1 and EMT in osteosarcoma cells.…”