Retracted: Effect of microRNA‐128 on cisplatin resistance of glioma SHG‐44 cells by targeting JAG1

Abstract

This current study intends to investigate the effect of microRNA-128 (miR-128) on cisplatin (DDP) resistance in glioma SHG-44 cells. SHG-44/DDP cells were transfected with miR-128 antisense oligonucleotide (ASO) and assigned into blank, resistance, NC, anti-miR-128, miR-128 mimic, si-JAG1, and anti-miR-128 + si-JAG1 groups. qRT-PCR and Western blotting were employed for determining expression of miR-128, JAG1, Bax and Bcl-2. MTT assay, Giemsa staining, and flow cytometry were applied to detect DDP resistance, cellular morphology, and cell cycle, respectively. JAG1 is targeted and negatively regulated by miR-128. In in vitro experiments, compared with the blank group, the rest groups exhibited declined miR-28 and Bax expression, lowered cell inhibition rate and apoptosis rate, but elevated JAG1 and Bcl-2 expression with cells arrested in the S phase. Compared with the resistance group, the anti-miR-128 group showed decreasedBax expression along with a lowered cell inhibition rate and apoptosis rate, but increased JAG1 and Bcl-2 expression with reduced cells arrested in the S phase; while the miR-128 mimic group showed an opposite trend; the si-JAG1 group showed decreased Bcl-2 expression and reduced cells in the S phase. In in vivo experiments, compared with the resistance group, the tumor growth rate, tumor volume, and weight as well as JAG1 expression accelerated in the anti-miR-128 group; whereas the miR-128 mimic and si-JAG1 groups exhibited an opposite trend. Our findings demonstrated that miR-128 ASO transfection might down-regulate the expression of miR-128 in SHG-44/DDP and up-regulate the DDP resistance in SHG-44/DDP cells, providing a potential treatment target for glioma.

<p>Long noncoding RNA DANCR contributes to docetaxel resistance in prostate cancer through targeting the miR-34a-5p/JAG1 pathway</p>

“…Moreover, upregulation of JAG1 oncogene is observed in nilotinib and imatinib-resistant myeloblastic leukemia cells 29. miR-26b and miR-128 contribute to the resistance of nasopharyngeal carcinoma or glioma to cisplatin by downregulating JAG1 30,31. Consistent with previous studies, we observed that knockdown of JAG1 enhanced the chemosensitivity of DTX-tolerated PC cells to DTX, and the biological function of JAG1 was rescued by miR-34a-5p inhibitor.…”

Long Non-coding RNA Maternally Expressed 3 Increases the Expression of Neuron-Specific Genes by Targeting miR-128-3p in All-Trans Retinoic Acid-Induced Neurogenic Differentiation From Amniotic Epithelial Cells

“…In cells transfected with pre-miR-128 and pRL-JAG1-WT, luciferase activity significantly decreased relative to that in cells cotransfected with the control precursor or mutated versions of the target (Figure 2D). These results demonstrate that miR-128 directly suppresses the expression of JAG1 through targeting seed sequences in the 3 -UTR of JAG1, in agreement with a previous report (Yi et al, 2018). To test the role of JAG1 in neurogenic differentiation, a small interfering RNA (siRNA) of JAG1 was designed, synthesized, and verified (Figure 2E).…”

Revisiting Platinum-Based Anticancer Drugs to Overcome Gliomas

“…However, resistance to chemotherapy occurs inside cancer cells, reducing treatment efficiency, and platinum-based chemotherapy is no exception [ 110 ]. Overcoming drug resistance by revealing the molecular mechanisms that mediate cisplatin resistance will have important clinical value in improving the treatment outcome of brain tumors [ 111 , 112 ]. Many research have been conducted on the mechanism of cisplatin resistance acquisition in brain tumor cells.…”