<i>Respiratory and Glycolytic Activities of Human Leukocytes in Vitro</i>

McKinney, Gordon R.; Martin, Samuel P.; Rundles, R. Wayne; Green, Rosalie

doi:10.1152/jappl.1953.5.7.335

Cited by 63 publications

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“…For lymphocytes, 64-8 + 31'9 These results are in agreement with published values (Esmann, 1962;Hedeskov & Esmann, 1966;McKinney, Martin, Rundles & Green, 1952;Rauch et al, 1961). Any differences are probably due to variations in the separation techniques and in the incubation media, particularly glucose content, used.…”

Section: Resultssupporting

confidence: 90%

The effects of some drugs which induce agranulocytosis on the metabolism of separated human polymorphonuclear leucocytes and lymphocytes

McCurrach

Park

Perry

1970

British J Pharmacology

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Summary . Lymphocytes and polymorphonuclear leucocytes were separated from normal human blood by the method of Rabinowitz (1964). . The oxygen uptake, lactic acid production and glucose uptake of suspensions of these cells in vitro were measured. The values obtained agree with published data using comparable techniques. . Amidopyrine, chloramphenicol, chlorpromazine, phenylbutazone and thiouracil, in high concentrations, depressed the oxygen uptake of polymorphs, but not that of lymphocytes. With amidopyrine, chlorpromazine and thiouracil significant inhibition was produced by concentrations equal to therapeutic plasma levels. High concentrations of the drugs, except chlorpromazine, stimulated the glucose uptake of lymphocytes but not that of polymorphs. Tetracycline and sulphisoxazole were without effect on either parameter of leucocyte metabolism. . Because of erythrocyte contamination the lactic acid production of lymphocytes could not be determined. Lactate production by polymorphs was not affected by any of the drugs studied. . The significance of these results is discussed. It is suggested that there is a relationship between ability to induce agranulocytosis and effect on leucocyte metabolism in vitro.

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Section: Resultssupporting

confidence: 90%

The effects of some drugs which induce agranulocytosis on the metabolism of separated human polymorphonuclear leucocytes and lymphocytes

McCurrach

Park

Perry

1970

British J Pharmacology

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“…The effect of compound S is the same as the response noted on injury (4). It may be a pharmacological effect or may be due to some factor present in the material such as pyrogen contamination (12).…”

Section: Resultsmentioning

confidence: 96%

“…The method for collection and preparation of blood has been previously described (4). Healthy donors in the fasting state were used.…”

Section: Methodsmentioning

confidence: 99%

“…or a cation exchange resin (Dowex-50) was used to prevent coagulation. The leukocytes were concentrated by the method previously described (4) In one group of subjects, the in vitro effect of cholesterol, cortisone, hydrocortisone, desoxycorticosterone, and Reichstein's compound S on lactic acid production was measured. In another group, the effect of cortisone on the production of lactate and the utilization of glucose, oxygen, and glycogen was measured by techniques previously described (4).…”

Section: Methodsmentioning

confidence: 99%

“…One of the important cells in the antibacterial defense and in the inflammatory response is the polymorphonuclear leukocyte. This cell has a very high production of lactic acid in the presence of oxygen and this rate is not altered significantly by the concentration of oxygen (4,5). The early chemical changes in an area of inflammation such as lowering of pH are in part due to the aerobic lactic acid production by leukocytes.…”

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confidence: 99%

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The Effect of Adrenal Steroids on Aerobic Lactic Acid Formation in Human Leukocytes1

Martin¹,

Chaudhuri²,

Green³

et al. 1954

J. Clin. Invest.

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Cortisone has an influence on the course of infectious diseases, as well as on the intensity of the inflammatory response (1-3). One of the important cells in the antibacterial defense and in the inflammatory response is the polymorphonuclear leukocyte. This cell has a very high production of lactic acid in the presence of oxygen and this rate is not altered significantly by the concentration of oxygen (4,5). The early chemical changes in an area of inflammation such as lowering of pH are in part due to the aerobic lactic acid production by leukocytes.The present study was undertaken to determine the effect of active and inactive adrenal steroids on this metabolic characteristic of human leukocytes in vitro. Compound E and F were found to inhibit aerobic lactic acid production. MATERIAL AND METHODSThe method for collection and preparation of blood has been previously described (4). Healthy donors in the fasting state were used. The blood was collected from the cubital veins into a non-wetting system. All glassware was siliconed (G.E. Silicone SC 77). Heparin (0.04 mg. per ml.) or a cation exchange resin (Dowex-50) was used to prevent coagulation. The leukocytes were concentrated by the method previously described (4) in which dextran (intrinsic viscosity 0.46) is added to produce a final concentration of 1 per cent. The dextran induced rouleaux formation and rapid sedimentation of red blood cells. After removal of the supernatant plasma containing the leukocytes, the cells were concentrated by centrifugation at 50 x G for 15 minutes. The plasma was decanted and the white cells were resuspended in a sufficient volume of buffered saline (4) with 100 mg. per cent glucose to obtain a concentration of 5,000 to 25,000 cells per cu.mm. Cortisone and hydrocortisone

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Uremia and Insulin Response

Westervelt¹

1970

Arch Intern Med

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Respiratory and Glycolytic Activities of Human Leukocytes in Vitro

Cited by 63 publications

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The effects of some drugs which induce agranulocytosis on the metabolism of separated human polymorphonuclear leucocytes and lymphocytes

The effects of some drugs which induce agranulocytosis on the metabolism of separated human polymorphonuclear leucocytes and lymphocytes

The Effect of Adrenal Steroids on Aerobic Lactic Acid Formation in Human Leukocytes1

Uremia and Insulin Response

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