Rehmannia glutinosapolysaccharide induces toll-like receptor 4 dependent spleen dendritic cell maturation and anti-cancer immunity

Abstract

polysaccharide (RGP) has shown an activation of immune cells . However, the immune stimulatory effect of RGP in a mouse is not well studied. In this study, we examined the effect of RGP on dendritic cell (DC) activation and anticancer immunity . Treatments of RGP in C56BL/6 mice induced increased levels of co-stimulatory molecule expression and pro-inflammatory cytokine production in spleen DCs dependent on toll-like receptor 4 (TLR4), and those DCs promoted interferon-gamma (IFNγ) production in CD4 and CD8 T cells. RGP also enhanced ovalbumin (OVA) antigen (Ag)-specific immune activation in tumor-bearing mice, including Ag presentation in DCs, OT-I and OT-II T-cell proliferation, migration of OT-I and OT-II T cells into the B16-OVA tumor, OVA-specific IFNγ production, and the specific killing of OVA-coated splenocytes, which consequently inhibited B16-OVA tumor growth dependent on TLR4 and CD8 T cells. Finally, the combination of RGP and self-Ag treatment efficiently inhibited CT26 carcinoma and B16 melanoma tumor growth in BLAB/c and C57BL/6 mice, respectively. These data demonstrate that RGP could be a useful adjuvant molecule for immunotherapy against cancer.

Cancer immunotherapy using a polysaccharide from Codium fragile in a murine model

“…Therefore, CFP may target not only CR3, but also other receptors, and contribute to DC activation. Fucoidan and ascophyllan bind to scavenger receptor-A (SR-A) and TLR4, respectively, 5,20,27 the CFP may target other pattern recognition receptors (PRRs). Further studies will investigate the properties of CFP in binding to these receptors on DCs using receptor knockout (KO) mice.…”

“…As described in previous studies, tumor-infiltrated cells were stimulated with 2 μg/mL Ag for 6 h and treated with 1 μg/mL Monensin Solution (Biolegend, San Diego, California, US; #420701) over the final 3 h. 5 Cells were harvested and stained with surface Abs for 15 min, followed by incubation in fixation buffer (Biolegend, San Diego, California, US; #420801) and permeabilization with Cytofix/Cytoperm buffer (eBioscience, San Diego, California, US; #426803). Cells were incubated with anti-cytokine Abs in Perm/Wash buffer (eBioscience, San Diego, California, US; #421002) for 30 min.…”

“…The immune system has the ability to recall Ags and may contribute, as a memory response, to the prevention of secondary exposures to the same Ags. 5 As many patients with cancer have metastasis and relapse after tumor removal, cancer Ag-specific immune activation may serve as a strategy for preventing cancer metastasis. Therapeutic cancer vaccines rely on Ag-specific immune responses, including activation of cytotoxic T lymphocytes (CTLs) and helper T (Th) cells, as well as production of antibodies (Abs) by memory cells to prevent cancer regeneration.…”

Lipopolysaccharide-coated CuS nanoparticles promoted anti-cancer and anti-metastatic effect by immuno-photothermal therapy

“…It has been well studied that treatment of stimulatory molecules promotes the alteration of DC numbers in the spleen and LN [ 24 , 25 , 27 , 41 ]. In case of LPS, it can induce full activation of DCs with the decreases in the DC numbers in the spleen and LN [ 24 , 25 ].…”

Puffing of Rehmannia glutinosa enhances anti-oxidant capacity and down-regulates IL-6 production in RAW 264.7 cells

“…The roots of Rehmannia glutinosa (RG), generally known as Jihwang in Korea, are widely used as an herb in traditional oriental medicine. Previous studies have shown that RG has anti-cancer (Xu et al, 2017a(Xu et al, , 2017b), blood-glucose regulatory (Zhang et al, 2004), anti-inflammatory (Wang et al, 2015), antioxidant (Zhang et al, 2004), and immunoregulatory (Kim et al, 1998) effects. Among the three forms in which RG is consumed, i.e., raw, dried, and steamed, the most commonly consumed form is 9-timesteamed RG (NSRG), which is prepared by repetition of alcohol soaking, steaming, and drying (Hong et al, 1993).…”