<i>RCS1</i>, a gene involved in controlling cell size in <i>Saccharomyces cerevisiae</i>

Gil, Rosario; Zueco, Jesús; Sentandreu, Rafael; Herrero, Enrique

doi:10.1002/yea.320070102

Cited by 16 publications

(21 citation statements)

References 47 publications

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“…This proved to be a fortuitous cross-reactivity. The authors of this study have named the gene RCS] because of the effects of mutation and disruption on cell size (Gil et al, 1991). While there are many sequence differences between our ORF and the corresponding region of RCS], we believe that these are due to polymorphisms and/or sequencing errors in the previous study.…”

Section: Isolation Of the Afti Genementioning

confidence: 79%

AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae.

1995

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Using a scheme for selecting mutants of Saccharomyces cerevisiae with abnormalities of iron metabolism, we have identified a gene, AFT1, that mediates the control of iron uptake. AFT1 encodes a 78 kDa protein with a highly basic amino terminal domain and a glutamine‐rich C‐terminal domain, reminiscent of transcriptional activators. The protein also contains an amino terminal and a C‐terminal region with 10% His residues. A dominant mutant allele of this gene, termed AFT1‐1up, results in high levels of ferric reductase and ferrous iron uptake that are not repressed by exogenous iron. The increased iron uptake is associated with enhanced susceptibility to iron toxicity. These effects may be explained by the failure of iron to repress transcription of FRE1, FRE2 and FET3. FRE1 and FRE2 encode plasma membrane ferric reductases, obligatory for ferric iron assimilation, and FET3 encodes a copper‐dependent membrane‐associated oxidase required for ferrous iron uptake. Conversely, a strain with interruption of the AFT1 gene manifests low ferric reductase and ferrous iron uptake and is susceptible to iron deprivation, because of deficient expression of FRE1 and negligible expression of FRE2 and FET3. Thus, AFT1 functions to activate transcription of target genes in response to iron deprivation and thereby plays a central role in iron homeostasis.

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Section: Isolation Of the Afti Genementioning

confidence: 79%

AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae.

1995

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“…Like RIM101 pathway mutants, aft1D cells are also sensitive to alkaline pH (Serrano et al 2004) and have sporulation defects (Gil et al 1991). Furthermore, microarray studies have shown that upon alkaline pH treatment, the expression levels of iron regulon genes are induced (Lamb et al 2001) presumably to compensate for decreases in iron availability in alkaline conditions (Serrano et al 2004).…”

Section: Resultsmentioning

confidence: 99%

“…The RIM101 pH pathway is known to be involved in numerous cellular processes: alkaline pH response (Hayashi et al 2005), sporulation (Su and Mitchell 1993;Li and Mitchell 1997), ion homeostasis (Lamb et al 2001), and cell-wall assembly (Castrejon et al 2006). Similarly, Aft1 has been implicated in alkaline pH response (Serrano et al 2004), sporulation (Gil et al 1991), cell wall ( Figure 2C), and ion homeostasis ( Figure 2C). Interestingly, while the aft1D cells' sensitivity to SDS, caffeine, and LiCl can be suppressed by exogenous iron, suggesting that iron is a cofactor for key proteins required for resistance to these treatments, exogenous iron cannot suppress the sensitivity of aft1D cells to CFW or NaCl.…”

Section: Discussionmentioning

confidence: 99%

Functional Genomics Analysis of theSaccharomyces cerevisiaeIron Responsive Transcription Factor Aft1 Reveals Iron-Independent Functions

et al. 2010

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The Saccharomyces cerevisiae transcription factor Aft1 is activated in iron-deficient cells to induce the expression of iron regulon genes, which coordinate the increase of iron uptake and remodel cellular metabolism to survive low-iron conditions. In addition, Aft1 has been implicated in numerous cellular processes including cell-cycle progression and chromosome stability; however, it is unclear if all cellular effects of Aft1 are mediated through iron homeostasis. To further investigate the cellular processes affected by Aft1, we identified .70 deletion mutants that are sensitive to perturbations in AFT1 levels using genome-wide synthetic lethal and synthetic dosage lethal screens. Our genetic network reveals that Aft1 affects a diverse range of cellular processes, including the RIM101 pH pathway, cell-wall stability, DNA damage, protein transport, chromosome stability, and mitochondrial function. Surprisingly, only a subset of mutants identified are sensitive to extracellular iron fluctuations or display genetic interactions with mutants of iron regulon genes AFT2 or FET3. We demonstrate that Aft1 works in parallel with the RIM101 pH pathway and the role of Aft1 in DNA damage repair is mediated by iron. In contrast, through both directed studies and microarray transcriptional profiling, we show that the role of Aft1 in chromosome maintenance and benomyl resistance is independent of its iron regulatory role, potentially through a nontranscriptional mechanism.

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“…The transcription factor Reb1 is known to bind with high affinity to a sequence upstream of CLB2 [38], a gene whose regulation is important for completion of the normal vegetative cell cycle. The regulator Rcs1 is involved in timing the budding event of the cell cycle [39]. Additional factors identified are Ash1, Sok2, Ste12 and Tec1.…”

Section: Resultsmentioning

confidence: 99%

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et al. 2006

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This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Network module discovery

ReMoDiscovery, a module discovery algorithm and software that uses ChIP-chip data, motif information and gene-expression profiles, is presented.

Abstract 'ReMoDiscovery' is an intuitive algorithm to correlate regulatory programs with regulators and corresponding motifs to a set of co-expressed genes. It exploits in a concurrent way three independent data sources: ChIP-chip data, motif information and gene expression profiles. When compared to published module discovery algorithms, ReMoDiscovery is fast and easily tunable. We evaluated our method on yeast data, where it was shown to generate biologically meaningful findings and allowed the prediction of potential novel roles of transcriptional regulators.

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RCS1, a gene involved in controlling cell size in Saccharomyces cerevisiae

Cited by 16 publications

References 47 publications

AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae.

AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae.

Functional Genomics Analysis of theSaccharomyces cerevisiaeIron Responsive Transcription Factor Aft1 Reveals Iron-Independent Functions

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