<i>rbohA</i>, a rice homologue of the mammalian <i>gp91phox</i> respiratory burst oxidase gene

Groom, Quentin; Torres, Miguel Ángel Medina; Fordham-Skelton, Anthony P.; Hammond‐Kosack, K. E.; Robinson, Nigel J.; Jones, Jonathan D. G.

doi:10.1046/j.1365-313x.1996.10030515.x

Cited by 278 publications

(148 citation statements)

References 0 publications

Supporting

Mentioning

146

Contrasting

Unclassified

Order By: Relevance

“…Both genomic DNA and cDNA from healthy leaves of A. thaliana ecotype Landsberg erecta were used as template in PCR with degenerate oligonucleotide primers, designed based on conserved regions between the Cterminus of the human gp91 phox protein (Royer-Pokora et al, 1986) and the rice gene OsrbohA (Groom et al, 1996). We identified PCR products corresponding to six different genes, named AtrbohA-F. Products from homologues D, E and F were recovered by amplification of a cDNA template, whereas products corresponding to homologues A, B and C were only amplified from genomic DNA template.…”

Section: Resultsmentioning

confidence: 99%

“…Further analysis of OsrbohA shows it is longer than initially reported. 5Ј to the methionine predicted by Groom et al (1996) lies an intron of Ͼ 6 kb that corresponds to the fourth intron of Atrboh genes. There is also a putative EF-hand motif in the third exon of OsrbohA (Groom and Torres, unpublished data).…”

Section: Resultsmentioning

confidence: 99%

“…We isolated OsrbohA, a rice gene homologous to gp91 phox (Groom et al, 1996). As Arabidopsis thaliana is widely used to study the plant defence response, we set out to document the entire A. thaliana Rboh gene family as a first step to generate mutants to assess the involvement of the Atrboh genes in the oxidative burst and in plant defence.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91^phox)

et al. 1998

Self Cite

View full text Add to dashboard Cite

SummaryAn NADPH oxidase analogous to that in mammalian phagocytes has been hypothesized to produce reactive oxygen species (ROS) in the plant defence response. A. thaliana contains at least six gp91 phox homologues, designated AtrbohA-F (A. thaliana Respiratory Burst Oxidase Homologues), which map to different positions. Transcripts of three of these genes can be detected in healthy plants by RNA gel blot analyses. The Atrboh gene products are closely related to gp91 phox and the intron locations suggest a common evolutionary origin. A putative EF-hand Ca 2⍣ -binding motif in the extended N-terminal region of the Atrboh proteins suggests a direct regulatory effect of Ca 2⍣ on the activity of the NADPH oxidase in plants.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91^phox)

et al. 1998

Self Cite

View full text Add to dashboard Cite

show abstract

“…Respiratory burst oxidase homologs (Rbohs), the plant homologs of GP91PHOX, are encoded by 10 genes in Arabidopsis thaliana (RbohA to RbohJ ) (3)(4)(5). Rbohs generate ROS in response to bacterial and fungal pathogens (3,6), and genetic disruption of specific Rbohs alters plant responses to pathogens (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

Parasitic Worms Stimulate Host NADPH Oxidases to Produce Reactive Oxygen Species That Limit Plant Cell Death and Promote Infection

et al. 2014

Self Cite

View full text Add to dashboard Cite

Plants and animals produce reactive oxygen species (ROS) in response to infection. In plants, ROS not only activate defense responses and promote cell death to limit the spread of pathogens but also restrict the amount of cell death in response to pathogen recognition. Plants also use hormones, such as salicylic acid, to mediate immune responses to infection. However, there are long-lasting biotrophic plant-pathogen interactions, such as the interaction between parasitic nematodes and plant roots during which defense responses are suppressed and root cells are reorganized to specific nurse cell systems. In plants, ROS are primarily generated by plasma membrane-localized NADPH (reduced form of nicotinamide adenine dinucleotide phosphate) oxidases, and loss of NADPH oxidase activity compromises immune responses and cell death. We found that infection of Arabidopsis thaliana by the parasitic nematode Heterodera schachtii activated the NADPH oxidases RbohD and RbohF to produce ROS, which was necessary to restrict infected plant cell death and promote nurse cell formation. RbohD-and RbohF-deficient plants exhibited larger regions of cell death in response to nematode infection, and nurse cell formation was greatly reduced. Genetic disruption of SID2, which is required for salicylic acid accumulation and immune activation in nematode-infected plants, led to the increased size of nematodes in RbohD-and RbohF-deficient plants, but did not decrease plant cell death. Thus, by stimulating NADPH oxidase-generated ROS, parasitic nematodes fine-tune the pattern of plant cell death during the destructive root invasion and may antagonize salicylic acid-induced defense responses during biotrophic life stages.

show abstract

“…Homologs of gp91 phox have been identified in several plant species [90][91][92]. The Arabidopsis genome encodes ten putative gp91 phox homologs, termed Respiratory burst oxidase homolog (Rboh) [93].…”

Section: Regulation Of Nadph Oxidasementioning

confidence: 99%

A RHOse by any other name: a comparative analysis of animal and plant Rho GTPases

et al. 2006

View full text Add to dashboard Cite

Rho GTPases are molecular switches that act as key regulators of a many cellular processes, including cell movement, morphogenesis, host defense, cell division and gene expression. Rho GTPases are found in all eukaryotic kingdoms. Plants lack clear homologs to conventional Rho GTPases found in yeast and animals; instead, they have over time developed a unique subfamily, ROPs, also known as RAC. The origin of ROP-like proteins appears to precede the appearance of land plants. This review aims to discuss the evolution of ROP/RAC and to compare plant ROP and animal Rho GTPases, focusing on similarities and differences in regulation of the GTPases and their downstream effectors.

show abstract

rbohA, a rice homologue of the mammalian gp91phox respiratory burst oxidase gene

Cited by 278 publications

References 0 publications

Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91^phox)

Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91^phox)

Parasitic Worms Stimulate Host NADPH Oxidases to Produce Reactive Oxygen Species That Limit Plant Cell Death and Promote Infection

A RHOse by any other name: a comparative analysis of animal and plant Rho GTPases

Contact Info

Product

Resources

About

rbohA, a rice homologue of the mammalian gp91phox respiratory burst oxidase gene

Cited by 278 publications

References 0 publications

Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91phox)

Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91phox)

Parasitic Worms Stimulate Host NADPH Oxidases to Produce Reactive Oxygen Species That Limit Plant Cell Death and Promote Infection

A RHOse by any other name: a comparative analysis of animal and plant Rho GTPases

Contact Info

Product

Resources

About

Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91^phox)

Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91^phox)