ras oncogene‐induced transformation of a rat seminal vesicle epithelial cell line produces a marked increase of adenylate cyclase and protein kinase C activities

Abstract

Cells transformedby Kirsten murine sarcoma virus (Ki-MSV) have basal adenylate cyclase activity (AC) higher than control cells and comparable level of forskohn-stimulated AC activity. Moreover, a htgher protem kinase C (PKC) activtty was found to be present in the transformed cells. The molecular mechanism underlying the Increase of AC activtty was mvesttgated. Our findmgs strongly suggest that this biochemtcal event is due to a marked decrease of the LX, negative control of the enzyme. even though the a, of transformed cells appears to possess fully functional domains Interacting wtth both the effector enzyme and the agonist-acttvated receptor.

Sodium butyrate/retinoic acid costimulation induces apoptosis-independent growth arrest and cell differentiation in normal and ras-transformed seminal vesicle epithelial cells unresponsive to retinoic acid

“…The SVC1 cells have been derived from rat seminal vesicle epithelium (Tajana et al 1984), while the Ki-SVC1 cells were obtained by transformation of the SVC1 cells with the Kirsten ras-containing virus (Spina et al 1993). Both cell types had a doubling time of about 20 h (Spina et al 1993) and were cultured as monolayers at 37 C in an humidified atmosphere of 5% CO 2 -95% air in Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% fetal calf serum, 2 mM glutamine, 100 U/ml penicillin, and 100 µg/ml streptomycin (standard culture medium).…”

“…Both cell types had a doubling time of about 20 h (Spina et al 1993) and were cultured as monolayers at 37 C in an humidified atmosphere of 5% CO 2 -95% air in Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% fetal calf serum, 2 mM glutamine, 100 U/ml penicillin, and 100 µg/ml streptomycin (standard culture medium). The medium was changed every two days and, when required, the cells were treated with all-trans RA (Sigma, St Louis, MO, USA), or NaB (Sigma) or a combination of RA and NaB, using appropriate dilutions in DMEM of the original stock solutions in dimethyl sulfoxide (DMSO), and without changing the medium from the beginning of treatment to the end of the experiments.…”

“…Following their establishment as an immortalized cell line, the SVC1 cells have been kept in culture for the past 15 years with a total of about 300 passages. During this time a typical differentiation feature of these cells, namely their ability to synthesize and secrete into the medium the protein SV-IV under androgen control, progressively decreased until it disappeared completely (Ostrowski et al 1979, Mansson et al 1981, Tajana et al 1984, Spina et al 1993.…”

“…The main morphological, biological, and biochemical characteristics of these cells have been described previously (Tajana et al 1984, Spina et al 1993. Following their establishment as an immortalized cell line, the SVC1 cells have been kept in culture for the past 15 years with a total of about 300 passages.…”

Porin fromPseudomonas aeruginosaInduces Apoptosis in an Epithelial Cell Line Derived from Rat Seminal Vesicles

“…On the basis of these data and considerations, and taking into account the scarce information available on the cytotoxic effects of very low concentrations of LPS or porin on specific target cells, we were prompted to investigate the possible involvement of these substances in the induction of apoptosis in a cell line (SVC1) derived from the rat seminal vesicle secretory epithelium (36,37,49).…”