<i>Raphanus sativus</i> L. seed extracts induce apoptosis and reduce migration of oral squamous cell carcinoma KB and KB<sup>CD133+</sup>cells by downregulation of β-catenin

Ahn, Kyuhyeon; Ji, Hyeongjoon; Kim, Hye-Eun; Cho, Hyejoung; Sun, Qiaochu; Shi, Shuhan; He, Yingkun; Kim, Byung-Gook; Kim, Ok-Su

doi:10.1080/01635581.2019.1684527

Cited by 8 publications

(6 citation statements)

References 34 publications

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“…ITCs are known to inhibit C6 glioma cell invasion and migration by inhibiting MMP‐9 expression mediated by FAK/JNK 44,45 According to Pawlik et al ., 46 sulforaphene led to an increase in Bax protein, whereas Bcl‐2 levels decreased. Our findings were similar to a previous study reporting that R. sativus L. seed extracts had the potential to trigger apoptosis and minimize oral squamous KB cell carcinoma by lowering MMP‐2 and MMP‐9 levels 47 . The molecular mechanisms leading to cancer cell death were also similar to those of R. sativus ‐induced apoptosis involving Bcl‐2 family gene interaction along with Caspase‐3 activation 38 …”

Section: Resultssupporting

confidence: 90%

“…Our findings were similar to a previous study reporting that R. sativus L. seed extracts had the potential to trigger apoptosis and minimize oral squamous KB cell carcinoma by lowering MMP-2 and MMP-9 levels. 47 The molecular mechanisms leading to cancer cell death were also similar to those of R. sativus-induced apoptosis involving Bcl-2 family gene interaction along with Caspase-3 activation. 38 www.soci.org V Luang-In et al…”

Section: Protein Expression Of Mmp-2 and Mmp-9 And Gene Expression Inmentioning

confidence: 85%

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Effect of cold plasma and elicitors on bioactive contents, antioxidant activity and cytotoxicity of Thai rat‐tailed radish microgreens

et al. 2020

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BACKGROUND: Raphanus sativus var. caudatus or Thai rat-tailed radish (RTR) contains glucosinolates and isothiocyanates with chemopreventive effects; however, only mature plants have been investigated to date. Thus, the present study aimed to determine isothiocyanates, phenolic compounds and flavonoid compounds, antioxidant activity, cytotoxicity, and antiproliferative activity of RTR microgreens grown from seeds treated with cold plasma (21 kV for 5 min), organic elicitor (160 mmol L −1 NaCl, 10 mmol L −1 CaCl 2 or 176 mmol L −1 sucrose) or both in combination. Seeds were germinated on vermiculite and sprayed with deionized water or elicitor for 7 days before harvest. RESULTS: Cold plasma had insignificant effect on growth, whereas NaCl and CaCl 2 increased fresh weight. Plasma with CaCl 2 led to the highest total isothiocyanate (ITC) content [1.99 g kg −1 dry weight (DW)] in RTR microgreens containing raphasatin as the only ITC detected. Plasma treatment gave the highest total phenolic content (7.56 mg gallic acid equivalents g −1 DW), antioxidant activity from a 2,2-diphenyl-1-picrylhydrazyl assay (7.70 mg trolox equivalents g −1 DW) and ferric reducing antioxidant power assay (21.72 mg Fe 2+ g −1 DW). Microgreen extracts from plasma showed an IC 50 value of 29.28 and 13.83 ∼g mL −1 towards MCF-7 and HepG2, respectively, with inhibitory properties on matrix metalloproteinase (MMP)-2 and MMP-9 proteins. Plasma enhanced Bax and Caspase-3 gene expression but reduced Bcl-2 and MMP-9 expression, indicating activation of apoptosis. CONCLUSION: Cold plasma shows promise as an innovative tool to enhance bioactive compounds with chemopreventive benefits in microgreens.

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Section: Resultssupporting

confidence: 90%

Section: Protein Expression Of Mmp-2 and Mmp-9 And Gene Expression Inmentioning

confidence: 85%

Effect of cold plasma and elicitors on bioactive contents, antioxidant activity and cytotoxicity of Thai rat‐tailed radish microgreens

et al. 2020

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“…The study confirmed potential use in folk medicine for control against urolithiasis [149]. Raphani semen (RS), the radish seed, plays a huge traditional medicinal role in China, India and South Korea as a laxative, for constipation and diarrhea prevention, coughs and distending pain, with antitumor, carminative, and anti-inflammatory effects and reduction in oral squamous cell carcinoma [150][151][152][153]. In summary, these studies demonstrate that R. raphanistrum subsp.…”

Section: Daikon Radish (Raphanus Raphanistrum Subsp Sativus)supporting

confidence: 53%

Antimicrobial Effects of Plant-Based Supplements on Gut Microbial Diversity in Small Ruminants

Daniel,

Njue,

Sanad

2023

Pathogens

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Every year in the United States, approximately 48 million people are affected by bacterial illnesses that are transmitted through food, leading to 3000 fatalities. These illnesses typically stem from food animals and their by-products, which may harbor dangerous pathogens like Salmonella enterica, Listeria monocytogenes, enterohemorrhagic Escherichia coli O157:H7, and Campylobacter jejuni. Factors that contribute to contamination include manure used as a soil amendment, exposure to polluted irrigation water, and contact with animals. To improve food safety, researchers are studying pre-slaughter intervention methods to eliminate bacterial contamination in live animals. While small ruminants are vital to global agriculture and income generation for small farms, traditional feeding practices involve supplements and antibiotics to boost performance, which contributes to antibiotic resistance. Hence, researchers are looking for friendly bacterial strains that enhance both animal and human health without impacting livestock productivity. The global trend is to minimize the use of antibiotics as feed supplements, with many countries prohibiting or limiting their use. The aim of this review is to provide a comprehensive insight on the antioxidant capabilities, therapeutic attributes, and applications of bioactive compounds derived from sweet potato tops (SPTs), rice bran (RB) and radish tops (RTs). This overview provides an insight on plant parts that are abundant in antioxidant and prebiotic effects and could be used as value-added products in animal feed and pharmaceutical applications. This review was based on previous findings that supplementation of basal diets with natural supplements represents a multifaceted intervention that will become highly important over time. By remarkably reducing the burden of foodborne pathogens, they apply to multiple species, are cheap, do not require withdrawal periods, and can be applied at any time in food animal production.

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“… •In addition, the p-GSK-3b level and p-GSK-3b/t-GSK-3b ratio were significantly decreased dose-dependently, leading to induced apoptosis. [ 3 ] 23 Orobanche crenata Methanol extract •Antioxidant •Cytotoxic •Induces apoptosis An in vitro study using hepatocellular carcinoma (HepG2), human prostate cancer (PC3), human breast adenocarcinoma (MCF-7), and human colon carcinoma (HCT-116) •The extract exhibited potent antioxidant activity. •The extract revealed a remarkable cytotoxic effect on HepG2, PC3, MCF-7, and HCT-116 cells with IC50 values of 30.3, 111, 89.6, and 28.6 mg/mL, respectively.…”

Section: Introductionmentioning

confidence: 99%

Natural products targeting inflammation-related metabolic disorders: A comprehensive review

Nainu

Frediansyah²,

Mamada

et al. 2023

Heliyon

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Raphanus sativus L. seed extracts induce apoptosis and reduce migration of oral squamous cell carcinoma KB and KB^CD133+cells by downregulation of β-catenin

Cited by 8 publications

References 34 publications

Effect of cold plasma and elicitors on bioactive contents, antioxidant activity and cytotoxicity of Thai rat‐tailed radish microgreens

Effect of cold plasma and elicitors on bioactive contents, antioxidant activity and cytotoxicity of Thai rat‐tailed radish microgreens

Antimicrobial Effects of Plant-Based Supplements on Gut Microbial Diversity in Small Ruminants

Natural products targeting inflammation-related metabolic disorders: A comprehensive review

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Raphanus sativus L. seed extracts induce apoptosis and reduce migration of oral squamous cell carcinoma KB and KBCD133+cells by downregulation of β-catenin

Cited by 8 publications

References 34 publications

Effect of cold plasma and elicitors on bioactive contents, antioxidant activity and cytotoxicity of Thai rat‐tailed radish microgreens

Effect of cold plasma and elicitors on bioactive contents, antioxidant activity and cytotoxicity of Thai rat‐tailed radish microgreens

Antimicrobial Effects of Plant-Based Supplements on Gut Microbial Diversity in Small Ruminants

Natural products targeting inflammation-related metabolic disorders: A comprehensive review

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Product

Resources

About

Raphanus sativus L. seed extracts induce apoptosis and reduce migration of oral squamous cell carcinoma KB and KB^CD133+cells by downregulation of β-catenin