<i>Ralstonia pickettii</i>in environmental biotechnology: potential and applications

Ryan, Michael P.; Pembroke, J. Tony; Adley, Catherine C.

doi:10.1111/j.1365-2672.2007.03361.x

Cited by 115 publications

(57 citation statements)

References 82 publications

(148 reference statements)

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“…It is an aerobic, non-fermentative, Gram-negative bacterium and ubiquitous in aqueous environments, soil, and plants. It has also been used in biotechnology applications (Ryan et al 2007) such as metabolizing the explosive RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) and degradation of acrylamide (Kitahara et al 2012). In a recent study, Arshi et al (2013) investigated that Aspergillus niger decolorized MB by 22.44% whereas T. lignorum can decolorized MB by 48.3%.…”

Section: Discussionmentioning

confidence: 99%

Isolation, screening and molecular identification of novel bacterial strain removing methylene blue from water solutions

Kilany

2017

Appl Water Sci

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The potentially deleterious effects of methylene blue (MB) on human health drove the interest in its removal promptly. Bioremediation is an effective and eco friendly for removing MB. Soil bacteria were isolated and examined for their potential to remove MB. The most potent bacterial candidate was characterized and identified using 16S rRNA sequence technique. The evolutionary history of the isolate was conducted by maximum likelihood method. Some physiochemical parameters were optimized for maximum decolorization. Decolorization mechanism and microbial toxicity study of MB (100 mg/l) and by-products were investigated. Participation of heat killed bacteria in color adsorption have been investigated too. The bacterial isolate was identified as Stenotrophomonas maltophilia strain Kilany_MB 16S ribosomal RNA gene with 99% sequence similarity. The sequence was submitted to NCBI (Accession number = KU533726). Phylogeny depicted the phylogenetic relationships between 16S ribosomal RNA gene, partial sequence (1442 bp), of the isolated strain and other strains related to Stenotrophomonas maltophilia in the GenBank database. The optimal conditions were investigated to be pH 5 at 30°C, after 24 h using 5 mg/l MB showing optimum decolorization percentage (61.3%). Microbial toxicity study demonstrated relative reduction in the toxicity of MB decolorized products on test bacteria. Mechanism of color removal was proved by both biosorption and biodegradation, where heat-killed and live cells showed 43 and 52% of decolorization, respectively, as a maximum value after 24-h incubation. It was demonstrated that the mechanism of color removal is by adsorption. Therefore, good performance of S maltophilia in MB color removal reinforces the exploitation of these bacteria in environmental clean-up and restoration of the ecosystem.

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Section: Discussionmentioning

confidence: 99%

Isolation, screening and molecular identification of novel bacterial strain removing methylene blue from water solutions

Kilany

2017

Appl Water Sci

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“…The sequences obtained from the DGGE bands showed that Betaproteobacteria, e.g. Ralstonia sp., which are capable of polyphenol degradation, were present (Steinle et al 1998, Ryan et al 2007.…”

Section: Bacterial Community Compositionmentioning

confidence: 99%

Community composition of bacterial biofilms on two submerged macrophytes and an artificial substrate in a pre-alpine lake

Hempel¹,

Grossart²,

Gross³

2009

Aquat. Microb. Ecol.

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We compared the heterotrophic community composition of bacterial biofilms on the submerged macrophytes Myriophyllum spicatum and Potamogeton perfoliatus and on an artificial surface in Lower Lake Constance (Germany) on spatial (plant age) and temporal scales using denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH). M. spicatum contains polyphenolic allelochemicals that inhibit algae, cyanobacteria, and heterotrophic bacteria, and possibly influence the community composition, whereas P. perfoliatus does not. In 2005, the community composition of bacterial biofilms on apices and leaves of M. spicatum differed significantly. In 2006, the biofilm communities on the apices or leaves of M. spicatum and P. perfoliatus and the artificial surface did not differ significantly, although all except one apex sample of M. spicatum formed a distinct cluster based on DGGE banding patterns. On all surfaces, members of the CytophagaFlavobacter-Bacteroidetes (CFB) group (16 to 22%), Alphaproteobacteria (19%), and Betaproteobacteria (7 to 31%) were abundant; Actinobacteria and Planctomycetes occurred less frequently. Sequences of DNA fragments excised from DGGE gels were mainly affiliated with yet uncultured clones originating from various freshwater habitats. Several sequences were from bacteria capable of degrading phenolic and aromatic compounds. The chemical composition of the 2 plant species and of the different parts of M. spicatum differed up to an order of magnitude. Differences in the biofilm community composition mainly depended on environmental factors (water level, conductivity, temperature, pH) and the plant chemical composition, especially the carbon and total phenolic content. Our results suggest that the biofilm community on M. spicatum apices is related to specific bacterial functions in this microenvironment.

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“…Metabolic degradation is less common, with only a few microorganisms being able to use dioxane as the sole source for carbon and energy (18,(30)(31)(32). Cometabolic pathways include several monooxygenase enzymes required for dioxane degradation, including the methane (MMO), propane (PrMO), phenol (PHE), tetrahydrofuran (THFMO), and toluene (TOL, T4MO, or RMO), monooxygenases (19,20,28,33,34). THFMO was proven to catalyze THF as well as dioxane biodegradation in Pseudonocardia sp.…”

mentioning

confidence: 99%

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Gedalanga

Pornwongthong

Mora³

et al. 2014

Appl Environ Microbiol

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Bacterial multicomponent monooxygenase gene targets in Pseudonocardia dioxanivorans CB1190 were evaluated for their use as biomarkers to identify the potential for 1,4-dioxane biodegradation in pure cultures and environmental samples. Our studies using laboratory pure cultures and industrial activated sludge samples suggest that the presence of genes associated with dioxane monooxygenase, propane monooxygenase, alcohol dehydrogenase, and aldehyde dehydrogenase are promising indicators of 1,4-dioxane biotransformation; however, gene abundance was insufficient to predict actual biodegradation. A time course gene expression analysis of dioxane and propane monooxygenases in Pseudonocardia dioxanivorans CB1190 and mixed communities in wastewater samples revealed important associations with the rates of 1,4-dioxane removal. In addition, transcripts of alcohol dehydrogenase and aldehyde dehydrogenase genes were upregulated during biodegradation, although only the aldehyde dehydrogenase was significantly correlated with 1,4-dioxane concentrations. Expression of the propane monooxygenase demonstrated a time-dependent relationship with 1,4-dioxane biodegradation in P. dioxanivorans CB1190, with increased expression occurring after over 50% of the 1,4-dioxane had been removed. While the fraction of P. dioxanivorans CB1190-like bacteria among the total bacterial population significantly increased with decrease in 1,4-dioxane concentrations in wastewater treatment samples undergoing active biodegradation, the abundance and expression of monooxygenase-based biomarkers were better predictors of 1,4-dioxane degradation than taxonomic 16S rRNA genes. This study illustrates that specific bacterial monooxygenase and dehydrogenase gene targets together can serve as effective biomarkers for 1,4-dioxane biodegradation in the environment.

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Ralstonia pickettiiin environmental biotechnology: potential and applications

Cited by 115 publications

References 82 publications

Isolation, screening and molecular identification of novel bacterial strain removing methylene blue from water solutions

Isolation, screening and molecular identification of novel bacterial strain removing methylene blue from water solutions

Community composition of bacterial biofilms on two submerged macrophytes and an artificial substrate in a pre-alpine lake

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

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