<i>Quorum sensing et quorum quenching</i> : Comment bloquer la communication des bactéries pour inhiber leur virulence ?

Mion, Sonia; Rémy, Benjamin; Plener, Laure; Chabrière, Éric; Daudé, David

doi:10.1051/medsci/2018310

Cited by 10 publications

(3 citation statements)

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“…It relies on the production and detection of small molecules known as autoinducers. QS enables the adaptation of bacterial behaviors in a cell density-dependent manner and allows bacteria to act cooperatively and induce different physiological functions (Mion et al, 2019). Among the mechanisms activated by QS, bioluminescence was the first described in Vibrio sp.…”

Section: Introductionmentioning

confidence: 99%

Lactonase-mediated inhibition of quorum sensing largely alters phenotypes, proteome, and antimicrobial activities in Burkholderia thailandensis E264

Gonzales

Plener

Armengaud

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionBurkholderia thailandensis is a study model for Burkholderia pseudomallei, a highly virulent pathogen, known to be the causative agent of melioidosis and a potential bioterrorism agent. These two bacteria use an (acyl-homoserine lactone) AHL-mediated quorum sensing (QS) system to regulate different behaviors including biofilm formation, secondary metabolite productions, and motility.MethodsUsing an enzyme-based quorum quenching (QQ) strategy, with the lactonase SsoPox having the best activity on B. thailandensis AHLs, we evaluated the importance of QS in B. thailandensis by combining proteomic and phenotypic analyses.ResultsWe demonstrated that QS disruption largely affects overall bacterial behavior including motility, proteolytic activity, and antimicrobial molecule production. We further showed that QQ treatment drastically decreases B. thailandensis bactericidal activity against two bacteria (Chromobacterium violaceum and Staphylococcus aureus), while a spectacular increase in antifungal activity was observed against fungi and yeast (Aspergillus niger, Fusarium graminearum and Saccharomyces cerevisiae).DiscussionThis study provides evidence that QS is of prime interest when it comes to understanding the virulence of Burkholderia species and developing alternative treatments.

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Section: Introductionmentioning

confidence: 99%

Lactonase-mediated inhibition of quorum sensing largely alters phenotypes, proteome, and antimicrobial activities in Burkholderia thailandensis E264

Gonzales

Plener

Armengaud

et al. 2023

Front. Cell. Infect. Microbiol.

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“…Depending on bacteria species, QS regulates the production of virulence factors, motilities, and/or biofilm formation. Thus, the disruption of QS signaling, also termed quorum quenching (QQ), appears as interesting adjuvant strategies in the fight against bacterial infections [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

African Plant-Based Natural Products with Antivirulence Activities to the Rescue of Antibiotics

et al. 2020

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The worldwide emergence of antibiotic-resistant bacteria and the thread of widespread superbug infections have led researchers to constantly look for novel effective antimicrobial agents. Within the past two decades, there has been an increase in studies attempting to discover molecules with innovative properties against pathogenic bacteria, notably by disrupting mechanisms of bacterial virulence and/or biofilm formation which are both regulated by the cell-to-cell communication mechanism called ‘quorum sensing’ (QS). Certainly, targeting the virulence of bacteria and their capacity to form biofilms, without affecting their viability, may contribute to reduce their pathogenicity, allowing sufficient time for an immune response to infection and a reduction in the use of antibiotics. African plants, through their huge biodiversity, present a considerable reservoir of secondary metabolites with a very broad spectrum of biological activities, a potential source of natural products targeting such non-microbicidal mechanisms. The present paper aims to provide an overview on two main aspects: (i) succinct presentation of bacterial virulence and biofilm formation as well as their entanglement through QS mechanisms and (ii) detailed reports on African plant extracts and isolated compounds with antivirulence properties against particular pathogenic bacteria.

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“…Quorum sensing (QS) is a communication system that many bacteria aggregates used to regulate their aggregate size via small molecules called autoinducers [1,2]. The process of interfering with QS through degradation of signals is termed as quorum quenching (QQ).…”

Section: Introductionmentioning

confidence: 99%

Activity Improvement and Vital Amino Acid Identification on the Marine-Derived Quorum Quenching Enzyme MomL by Protein Engineering

et al. 2019

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MomL is a marine-derived quorum-quenching (QQ) lactonase which can degrade various N-acyl homoserine lactones (AHLs). Intentional modification of MomL may lead to a highly efficient QQ enzyme with broad application potential. In this study, we used a rapid and efficient method combining error-prone polymerase chain reaction (epPCR), high-throughput screening and site-directed mutagenesis to identify highly active MomL mutants. In this way, we obtained two candidate mutants, MomLI144V and MomLV149A. These two mutants exhibited enhanced activities and blocked the production of pathogenic factors of Pectobacterium carotovorum subsp. carotovorum (Pcc). Besides, seven amino acids which are vital for MomL enzyme activity were identified. Substitutions of these amino acids (E238G/K205E/L254R) in MomL led to almost complete loss of its QQ activity. We then tested the effect of MomL and its mutants on Pcc-infected Chinese cabbage. The results indicated that MomL and its mutants (MomLL254R, MomLI144V, MomLV149A) significantly decreased the pathogenicity of Pcc. This study provides an efficient method for QQ enzyme modification and gives us new clues for further investigation on the catalytic mechanism of QQ lactonase.

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Quorum sensing et quorum quenching : Comment bloquer la communication des bactéries pour inhiber leur virulence ?

Abstract: Quorum sensing et quorum quenching : Comment bloquer la communication des bactéries pour inhiber leur virulence ?. médecine/sciences, EDP Sciences, 2019, 35 (1), pp.

Cited by 10 publications

References 43 publications

Lactonase-mediated inhibition of quorum sensing largely alters phenotypes, proteome, and antimicrobial activities in Burkholderia thailandensis E264

Lactonase-mediated inhibition of quorum sensing largely alters phenotypes, proteome, and antimicrobial activities in Burkholderia thailandensis E264

African Plant-Based Natural Products with Antivirulence Activities to the Rescue of Antibiotics

Activity Improvement and Vital Amino Acid Identification on the Marine-Derived Quorum Quenching Enzyme MomL by Protein Engineering

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