<i>pyewacket</i>, a new zebrafish fin pigment pattern mutant

Mellgren, Eve M.; Johnson, Stephen L.

doi:10.1111/j.1600-0749.2006.00311.x

Cited by 14 publications

(8 citation statements)

References 21 publications

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“…However, csf1r appears to be more completely down-regulated than pax7 or xdh in head NC cells of pax3 morphants. As two distinct populations of xanthophores have been described in adult zebrafish (Mellgren and Johnson, 2006), our data raise the possibility that the residual xanthophores of the head constitute a distinct population, ones that require little or no pax3 and csf1r activity.…”

Section: Discussionmentioning

confidence: 54%

Sequential actions of Pax3 and Pax7 drive xanthophore development in zebrafish neural crest

Minchin

Hughes

2008

Developmental Biology

140

144

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The Pax3/7 gene family has a fundamental and conserved role during neural crest formation. In people, PAX3 mutation causes Waardenburg syndrome, and murine Pax3 is essential for pigment formation. However, it is unclear exactly how Pax3 functions within the neural crest. Here we show that pax3 is expressed before other pax3/7 members, including duplicated pax3b, pax7 and pax7b genes, early in zebrafish neural crest development. Knockdown of Pax3 protein by antisense morpholino oligonucleotides results in defective fate specification of xanthophores, with complete ablation in the trunk. Other pigment lineages are specified and differentiate. As a consequence of xanthophore loss, expression of pax7, a marker of the xanthophore lineage, is reduced in neural crest. Morpholino knockdown of Pax7 protein shows that Pax7 itself is dispensable for xanthophore fate specification, although yellow pigmentation is reduced. Loss of xanthophores after reduction of Pax3 correlates with a delay in melanoblast differentiation followed by significant increase in melanophores, suggestive of a Pax3-driven fate switch within a chromatophore precursor or stem cell. Analysis of other neural crest derivatives reveals that, in the absence of Pax3, the enteric nervous system is ablated from its inception. Therefore, Pax3 in zebrafish is required for specification of two specific lineages of neural crest, xanthophores and enteric neurons.

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Section: Discussionmentioning

confidence: 54%

Sequential actions of Pax3 and Pax7 drive xanthophore development in zebrafish neural crest

Minchin

Hughes

2008

Developmental Biology

140

144

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“…Yet, chromatophores on the fins are of considerable interest for at least two reasons. First, some mutants exhibit a decoupling of body and fin patterns, with one developing normally despite defects in the other (Lang et al., ; Mellgren and Johnson, ; Parichy et al., ) (Figure A, B). This suggests either that body and fin micro‐environments differentially regulate chromatophore development, or that body and fins are populated by different chromatophore precursors having distinct genetic requirements.…”

Section: Stem Cells Contributing To Fin Pigment Pattern and Regenerationmentioning

confidence: 99%

Origins of adult pigmentation: diversity in pigment stem cell lineages and implications for pattern evolution

Parichy

Spiewak

2014

Pigment Cell Melanoma Res

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Summary Teleosts comprise about half of all vertebrate species and exhibit an extraordinary diversity of adult pigment patterns that function in shoaling, camouflage and mate choice and have played important roles in speciation. Here, we review recent studies that have identified several distinct neural crest lineages, with distinct genetic requirements, that give rise to adult pigment cells in fishes. These lineages include post-embryonic, peripheral nerve associated stem cells that generate black melanophores and iridescent iridophores, cells derived directly from embryonic neural crest cells that generate yellow-orange xanthophores, and bipotent stem cells that generate both melanophores and xanthophores. This complexity in adult chromatophore lineages has implications for our understanding of adult traits, melanoma, and the evolutionary diversification of pigment cell lineages and patterns.

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“…Interestingly, brighter light strengthened orange color on fish fins. In zebrafish, a mutant has been identified controlling the interaction of xanthophores and melanocytes to form the pigment pattern of the adult zebrafish fin (Mellgren and Johnson 2006). In anurans, light‐sensitive melanophores were found on the tail fin of Xenopus tadpoles (Moriya et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Effect of Light Intensity on Color Performance of False Clownfish, Amphiprion ocellaris Cuvier

Yasir¹,

Qin²

2009

J World Aquaculture Soc

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Color performance of false clownfish, Amphiprion ocellaris Cuvier, was examined under three levels of light intensity (20–50 , 600–850 , and 2700–3500 lx) for 5 wk. The experiment was conducted in nine rectangular glass aquaria (25 × 25 × 20 cm) with three replicates. Each aquarium was stocked with 36 fish, and 3 fish were randomly sampled from each aquarium every other week. Digital images were taken weekly on each individual fish after it was anesthetized in MS‐222. The color performance in hue, saturation, and brightness was quantified using image analysis. In addition to the whole‐body analysis, each fish image was divided into ventral and dorsal parts to assess the body position‐dependent effect. Furthermore, color differences between dorsal fin, anal fin, ventral fin, and caudal fin were also quantified. The whole body was brighter at low light than at medium or at high light intensity. Irrespective of light intensity, the dorsal side was more orange but less bright than the ventral side. Brighter light strengthened overall orange color on fish fins. The dorsal fin and ventral fins appeared more orange than the anal and caudal fins regardless of light intensity and exposure duration. Similar to body color, low light also led to brighter fins, especially for caudal and dorsal fins. Our results indicate that ambient light could regulate fish color performance but could not change the pigment dominance by β‐carotene. Light intensity is unlikely to change the contrast between dorsal and ventral sides, but dim light tends to make fish body brighter, and bright light strengthens orange color on fins.

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pyewacket, a new zebrafish fin pigment pattern mutant

Cited by 14 publications

References 21 publications

Sequential actions of Pax3 and Pax7 drive xanthophore development in zebrafish neural crest

Sequential actions of Pax3 and Pax7 drive xanthophore development in zebrafish neural crest

Origins of adult pigmentation: diversity in pigment stem cell lineages and implications for pattern evolution

Effect of Light Intensity on Color Performance of False Clownfish, Amphiprion ocellaris Cuvier

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