<i>Ptk2b</i> deletion improves mice folliculogenesis and fecundity via inhibiting follicle loss mediated by Erk pathway

Cong, Yanyan; Wu, Haibo; Bian, Xiaolan; Xie, Qin; Lyu, Qifeng; Cui, Junqi; Suo, Lun; Kuang, Yanping

doi:10.1002/jcp.29914

Cited by 5 publications

(5 citation statements)

References 49 publications

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“…The physiological role of the FOXO3a in the primordial follicle is to prevent oocytes from premature activation. However, when Bax and Ptk2b were knocked out, the reserve of primordial follicles in the ovary increased and the fertility of mice was prolonged 35,36 . We also found that when Sp1 was knocked out in GCs, the depletion of primordial follicles was reduced.…”

Section: Discussionmentioning

confidence: 64%

“…However, when Bax and Ptk2b were knocked out, the reserve of primordial follicles in the ovary increased and the fertility of mice was prolonged. 35,36 We also found that when Sp1 was knocked out in GCs, the depletion of primordial follicles was reduced. However, more growing follicles in the aging GC-Sp1 −/− mice ovary based on this study indicated that the surviving primordial follicles developed normally and that the fertility was prolonged.…”

Section: Discussionmentioning

confidence: 66%

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SP1 impacts the primordial to primary follicle transition by regulating cholesterol metabolism in granulosa cells

et al. 2023

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The primordial to primary follicle transition (PPT) in the ovary is critical to maintain sustainable reproductive resources in female mammals. However, it is unclear how granulosa cells (GCs) of the primary follicle participate in regulating PPT. This study focused on exploring the role of transcription factor Sp1 (SP1) in regulating PPT based on the fact that SP1 is pivotal for pregranulosa cell proliferation before primordial follicle formation. The results showed that mice fertility was prolonged when Sp1 was specifically depleted from GCs (GC-Sp1 −/− ).Besides, the PPT in GC-Sp1 −/− mice was reduced, resulting in more primordial follicles being preserved. Single-cell RNA-seq also indicated that the level of cholesterol metabolism was downregulated in GC-Sp1 −/− mice. Additionally, the PPT was promoted by either overexpression of ferredoxin-1 (FDX1), one of the key genes in mediating cholesterol metabolism or supplementing cholesterol for cultured fetal ovaries. Collectively, SP1 in GCs participates in the metabolism of cholesterol partially by regulating the transcription of Fdx1 during the PPT.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 66%

SP1 impacts the primordial to primary follicle transition by regulating cholesterol metabolism in granulosa cells

et al. 2023

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“…Protein-tyrosine kinase 2 beta ( PTK2B ) is a member of the focal adhesion kinase family and plays a key role in oocyte maturation and in vitro fertilization [ 41 , 42 ]. Sharma et al found that PTK2B is expressed in zebrafish oocytes and affects zebrafish reproduction [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…Meng et al found that PTK2B can mediate the process of early embryonic development in mice [ 42 ]. It was reported that PTK2B regulates female fertility by regulating the process of ovarian and follicle formation, and when it is inhibited, oocyte maturation and ovarian development are affected [ 41 ]. In addition, PTK2B was found to be involved in the process of human sperm capacitation [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of the Ovaries of Taihe Black-Bone Silky Fowls at Different Egg-Laying Stages

Xiang

Huang

Wang³

et al. 2022

Genes

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The poor egg-laying performance and short peak egg-laying period restrict the economic benefits of enterprises relating to the Taihe black-bone silky fowl. Ovaries are the main organ for egg production in poultry. Unlike that of mammals, the spawning mechanism of poultry has rarely been reported. As a prominent local breed in China, the reproductive performance of Taihe black-bone silky fowls is in urgent need of development and exploitation. To further explore the egg-laying regulation mechanism in the different periods of Taihe black-bone silky fowls, the ovarian tissues from 12 chickens were randomly selected for transcriptome analysis, and 4 chickens in each of the three periods (i.e., the pre-laying period (102 days old, Pre), peak laying period (203 days old, Peak), and late laying period (394 days old, Late)). A total of 12 gene libraries were constructed, and a total of 9897 differential expression genes (DEGs) were identified from three comparisons; the late vs. peak stage had 509 DEGs, the pre vs. late stage had 5467 DEGs, and the pre vs. peak stage had 3921 DEGs (pre-stage: pre-egg-laying period (102 days old), peak-stage: peak egg-laying period (203 days old), and late-stage: late egg-laying period (394 days old)). In each of the two comparisons, 174, 84, and 2752 differentially co-expressed genes were obtained, respectively, and 43 differentially co-expressed genes were obtained in the three comparisons. Through the analysis of the differential genes, we identified some important genes and pathways that would affect reproductive performance and ovarian development. The differential genes were LPAR3, AvBD1, SMOC1, IGFBP1, ADCY8, GDF9, PTK2B, PGR, and CD44, and the important signaling pathways included proteolysis, extracellular matrices, vascular smooth muscle contraction, the NOD-like receptor signaling pathway and the phagosome. Through the analysis of the FPKM (Fragments Per Kilobase of exon model per Million mapped fragments) values of the genes, we screened three peak egg-laying period-specific expressed genes: IHH, INHA, and CYP19A1. The twelve genes and five signaling pathways mentioned above have rarely been reported in poultry ovary studies, and our study provides a scientific basis for the improvement of the reproductive performance in Taihe black-bone silky fowls.

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“…Besides, other PFR-improving strategies beyond inhibition of mTOR activators, such as activating the SIRT1/FOXO1 signaling through hADSC-secreted HGF and bFGF (19) or up-regulating erk phosphorylation through Ptk2b knockout (20), could not specifically target ovaries as well and carry the risk of tumorigenesis (21,22).…”

Section: Introductionmentioning

confidence: 99%

Inhibiting bridge integrator 2 phosphorylation leads to improved oocyte quality, ovarian health and fertility in aging and after chemotherapy in mice

et al. 2021

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Female ovary is the earliest degenerated organ and it faces distinct medical disadvantages that impair primordial follicle reserve and oocyte quality. Herein, we found that bridge integrator 2 (Bin2) was predominant within mouse ovaries and oocytes, and global-knockout of Bin2 improved both female fertility and oocyte quality with healthy physiology in mice. Ovarian quantitative proteomics and phosphomics showed that Bin2 knockout specifically decreased only p-RPS6 of mTOR pathway; meanwhile, it increased nicotinamide nucleotide transhydrogenase (NNT), the free-radical detoxifier, over 6-fold. Mechanically, phosphorylation at Thr423 & Ser424 translocated membrane Bin2 into cytoplasm to phosphorylate RPS6, while p-RPS6 bound 42-95 bp NNT UTR to inhibit NNT translation. We then synthesized a peptide (BPP) to mimic Bin2 inhibition, and found that 3-week BPP injection improved primordial follicle reserve and oocyte quality in aging or chemotherapeutics-treated mice. In all, Bin2 inhibition improve both primordial follicle reserve and oocyte quality without discernible side effects.

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Ptk2b deletion improves mice folliculogenesis and fecundity via inhibiting follicle loss mediated by Erk pathway

Cited by 5 publications

References 49 publications

SP1 impacts the primordial to primary follicle transition by regulating cholesterol metabolism in granulosa cells

SP1 impacts the primordial to primary follicle transition by regulating cholesterol metabolism in granulosa cells

Transcriptome Analysis of the Ovaries of Taihe Black-Bone Silky Fowls at Different Egg-Laying Stages

Inhibiting bridge integrator 2 phosphorylation leads to improved oocyte quality, ovarian health and fertility in aging and after chemotherapy in mice

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