Pseudomonas syringaepv.tomatoDC3000 induces defense responses in diverse maize inbred lines

Abstract

Many phytopathogens translocate virulence (effector) proteins into plant cells to circumvent host immune responses during infection. One such pathogen isPseudomonas syringaepv.tomatoDC3000, which secretes at least twenty-nine effectors into host cells, of which a subset elicits host defense responses in crop plant species. However, it is unknown whetherP. syringaepv.tomatoDC3000 activates immune responses in diverse maize inbreds. Here, we screened a diverse maize germplasm collection for effector-dependent recognition of this bacterial pathogen. As a control, we infiltratedPseudomonas syringaeDC3000(D36E), a derivative ofP. syringaepv.tomatoDC3000 that lacks all endogenous effectors. In our evaluations, we observed a variety of responses toP. syringaepv.tomatoDC3000 in maize and scored the phenotypes as either no observable response (N) or as one of three responses: weak chlorosis (WC), chlorosis (C) with minimal cell death, and hypersensitive reaction (HR)-like cell death. Of the twenty-six maize inbreds screened, 13 were scored as N, 2 as WC, 2 as C, and 9 as HR-like cell death. Importantly, no maize line responded toP. syringaeDC3000(D36E), demonstrating the responses observed are likely dependent upon recognition of one or morePseudomonaseffectors. Importantly, maize inbreds that recognizeP. syringaepv.tomatoDC3000 accumulated detectable hydrogen peroxide as well as an increase in transcript expression of a subset of maize defense genes. Collectively, our results will likely stimulate new research aimed at identifying the cognate maize disease resistance proteins that recognize the activities of one or more bacterial effectors.

Genome-informed trophic classification and functional characterization of virulence proteins from the maize tar spot pathogenPhyllachora maydis

“…tomato DC3000 is an effective heterologous expression system for delivering non-bacterial effectors into host cells (Fabro et al, 2011; Sohn et al, 2007). Recent work by Jaiswal and Helm (2023) showed P. syringae pv. tomato DC3000 induces defense responses in diverse maize inbreds, including hydrogen peroxide accumulation and an increase in transcript expression of maize defense genes.…”