<i>Pseudomonas syringae</i> effector AvrE associates with plant membrane nanodomains and binds phosphatidylinositides i<i>n vitro</i>

X, Xin; Kinch, Lisa N.; Cai, Baochang; Bc, Paasch; Kvitko, Brian H.; Nv, Grishin; Sy, He

doi:10.1101/2021.07.08.451616

Cited by 3 publications

(1 citation statement)

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“…A protein pull-down assay using transgenic Arabidopsis expressing DEX::His-YFP-AvrE coupled with mass spectrometry identified several families of plant proteins that interact with AvrE (Xin et al, 2021). Interestingly, members of the type one protein phosphatase (TOPP) family, including TOPP1, TOPP2, and TOPP5, were detected in the complex (Xin et al, 2021). TOPPs are highly conserved across the eukaryotic kingdom (Uhrig et al, 2013), and there are nine members in the Arabidopsis genome (Figure 1A).…”

Section: Water-soaking-inducing Avre Interacts With Arabidopsis Topp ...mentioning

confidence: 99%

Bacterial effectors manipulate plant abscisic acid signaling for creation of an aqueous apoplast

Ding

Cai

et al. 2022

Cell Host & Microbe

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Section: Water-soaking-inducing Avre Interacts With Arabidopsis Topp ...mentioning

confidence: 99%

Bacterial effectors manipulate plant abscisic acid signaling for creation of an aqueous apoplast

Ding

Cai

et al. 2022

Cell Host & Microbe

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Bacterial pathogens deliver water- and solute-permeable channels to plant cells

Nomura,

Andreazza,

Cheng

et al. 2023

Nature

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Many animal- and plant-pathogenic bacteria use a type III secretion system to deliver effector proteins into host cells1,2. Elucidation of how these effector proteins function in host cells is critical for understanding infectious diseases in animals and plants3–5. The widely conserved AvrE-family effectors, including DspE in Erwinia amylovora and AvrE in Pseudomonas syringae, have a central role in the pathogenesis of diverse phytopathogenic bacteria6. These conserved effectors are involved in the induction of ‘water soaking’ and host cell death that are conducive to bacterial multiplication in infected tissues. However, the exact biochemical functions of AvrE-family effectors have been recalcitrant to mechanistic understanding for three decades. Here we show that AvrE-family effectors fold into a β-barrel structure that resembles bacterial porins. Expression of AvrE and DspE in Xenopus oocytes results in inward and outward currents, permeability to water and osmolarity-dependent oocyte swelling and bursting. Liposome reconstitution confirmed that the DspE channel alone is sufficient to allow the passage of small molecules such as fluorescein dye. Targeted screening of chemical blockers based on the predicted pore size (15–20 Å) of the DspE channel identified polyamidoamine dendrimers as inhibitors of the DspE/AvrE channels. Notably, polyamidoamines broadly inhibit AvrE and DspE virulence activities in Xenopus oocytes and during E. amylovora and P. syringae infections. Thus, we have unravelled the biochemical function of a centrally important family of bacterial effectors with broad conceptual and practical implications in the study of bacterial pathogenesis.

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Bacterial pathogens deliver water/solute-permeable channels as a virulence strategy

Nomura

Andreazza

Cheng

et al. 2023

Preprint

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Many animal and plant pathogenic bacteria utilize a type III secretion system to deliver effector proteins into the host cell. Elucidation of how these effector proteins function in the host cell is critical for understanding infectious diseases in animals and plants. The widely conserved AvrE/DspE-family effectors play a central role in the pathogenesis of diverse phytopathogenic bacteria. These conserved effectors are involved in the induction of water-soaking and host cell death that are conducive to bacterial multiplication in infected tissues. However, the exact biochemical functions of AvrE/DspE-family effectors have been recalcitrant to mechanistic understanding for three decades. Here we show that AvrE/DspE-family effectors fold into a β-barrel structure that resembles bacterial porins. Expression of AvrE and DspE in Xenopus oocytes results in (i) inward and outward currents, (ii) permeability to water and (iii) osmolarity-dependent oocyte swelling and bursting. Liposome reconstitution confirmed that the DspE channel alone is sufficient to allow the passage of small molecules such as fluorescein dye. Targeted screening of chemical blockers based on the predicted pore size (15-20 Å) of the DspE channel identified polyamidoamine (PAMAM) dendrimers as inhibitors of the DspE/AvrE channels. Remarkably, PAMAMs broadly inhibit AvrE/DspE virulence activities in Xenopus oocytes and during Erwinia amylovora and Pseudomonas syringae infections. Thus, we have unraveled the enigmatic function of a centrally important family of bacterial effectors with significant conceptual and practical implications in the study of bacterial pathogenesis.

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Pseudomonas syringae effector AvrE associates with plant membrane nanodomains and binds phosphatidylinositides in vitro

Cited by 3 publications

References 47 publications

Bacterial effectors manipulate plant abscisic acid signaling for creation of an aqueous apoplast

Bacterial effectors manipulate plant abscisic acid signaling for creation of an aqueous apoplast

Bacterial pathogens deliver water- and solute-permeable channels to plant cells

Bacterial pathogens deliver water/solute-permeable channels as a virulence strategy

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