Pseudomonas putida KT2440 is naturally endowed to withstand industrial‐scale stress conditions

Abstract

Pseudomonas putida is recognized as a very promising strain for industrial application due to its high redox capacity and frequently observed tolerance towards organic solvents. In this research, we studied the metabolic and transcriptional response of P. putida KT2440 exposed to large-scale heterogeneous mixing conditions in the form of repeated glucose shortage. Cellular responses were mimicked in an experimental setup comprising a stirred tank reactor and a connected plug flow reactor. We deciphered that a stringent response-like transcriptional regulation programme is frequently induced, which seems to be linked to the intracellular pool of 3-hydroxyalkanoates (3-HA) that are known to serve as precursors for polyhydroxyalkanoates (PHA). To be precise, P. putida is endowed with a survival strategy likely to access cellular PHA, amino acids and glycogen in few seconds under glucose starvation to obtain ATP from respiration, thereby replenishing the reduced ATP levels and the adenylate energy charge. Notably, cells only need 0.4% of glucose uptake to build those 3-HA-based energy buffers. Concomitantly, genes that are related to amino acid catabolism and b-oxidation are upregulated during the transient absence of glucose. Furthermore, we provide a detailed list of transcriptional short-and long-term responses that increase the cellular maintenance by about 17% under the industrial-like conditions tested.

Evaluation of chromosomal insertion loci in the Pseudomonas putida KT2440 genome for predictable biosystems design

“…Pseudomonas putida has become a desirable target organism for the production of industrial products due to several attributes of its native physiology ( Ankenbauer et al., 2020 ). P. putida has a naturally diverse catabolism, with the capacity to metabolize aliphatic, aromatic and heterocyclic compounds in addition to glucose ( Schmid et al., 2001 ), lending to the potential to metabolize carbon from complex feedstocks for commercial chemical production.…”

Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and Recovery

“…During HAA production with 1.25 kg, about twice the mass of foamed liquid was separated compared to RL production. As a generally higher foaming capability for RLs was determined previously [ 20 , 47 ], it is assumed that other components in the culture broth promote foam formation. Mainly cells, lysed cells, and secreted proteins are known to increase the foaming of a culture broth [ 34 , 73 ].…”

“…Such installations cannot avoid inhomogeneities in the reactor headspace and external pipelines. Here, again, P. putida stands out as a robust microbial cell factory, e.g., capable of enduring glucose limitations and temperature variations [ 47 , 48 ].…”

Transcriptional profiling of the stringent response mutant strain E. coli SR reveals enhanced robustness to large‐scale conditions

“…The basic technical setup has been characterized previously (Löffler et al, 2016;Simen et al, 2017). Minor modifications to the original setup have been made and are described elsewhere (Ankenbauer et al, 2020).…”