Pseudomonas Septicemia in Neutropenic Dogs I. Treatment with Granulocyte Transfusions

Abstract

A canine model wan developed for the study of Pseudomonar septicemia in the leukopenic host.

Chemiluminescence of Human and Canine Polymorphonuclear Leukocytes in the Absence of Phagocytosis

“…The donor was treated with a single intravenous dose of cyclophosphamide (40 mg/kg) and 3 days later the peripheral blood cells were used in a CL experiment with NaF. We have previously used cyclophosphamide in dogs, as a method of producing transient bone marrow suppression (23), and have found that 3 days after cyclophosphamide treatment, mononuclear leukocyte concentration is markedly depressed whereas PMNs, although depressed in total numbers, remain viable. A donor was evaluated in this study that had 99% PMNs and 1% mononuclear cells.…”

Disseminated Candidiasis in Leukopenic Dogs

“…Modest reductions in initial blood clearance rates in leukopenic dogs make definitive conclusions regarding the pathophysiologic participation of granulocytes and/or the macrophage system diffi-cult to separate at present. However, it has been demonstrated in the cyclophosphamide treated dog that granulocytopenia is crucial in predisposing to spontaneous or induced infections, and that granulocyte transfusions alone are beneficial (9,10). Studies of replacement therapy (e.g., granulocyte transfusions) will be of interest in the present model.…”

“…Mongrel dogs weighrng between 15-20 kg and immunized against hepatitis and distemper were observed for two weeks prior to use. Leukopenia was produced by a single iv injection of cyclophosphamide (50 mg per kg body weight; courtesy of Mead Johnson Laboratories, Evansville, IN) as previously described (9). Dogs were fasted overnight prior to cyclophosphamide treatment and were given saline subcutaneously as needed for vomiting and dehydration.…”

Supportive Therapie der Knochenmarksinsuffizienz Technische, immunologische und klinische Entwicklungen

“…Untersuchungen an granulocytopenischen Tieren lieBen erkennen, dab Transfusionen yon Granulocyten den Verlauf inokulierter Septikfimien zu findern ver-m6gen [25,33,34,89]. In einer Studie an Hunden mit strahleninduzierter Granulocytopenie wurde der Wert prophylaktisch gegebener Granulocytentransfusionen als Zusatzbehandlung zu Gentamycin bei inokulierter Pseudomonas-Pneumonien demonstriert [221.…”