<i>Pseudomonas</i>Plasmids

Thomas, Christopher M.; Haines, Anthony S.; Кошелева, И. А.; Boronin, A. M.

doi:10.1002/9783527622009.ch11

Cited by 4 publications

(2 citation statements)

References 185 publications

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“…An in-depth bioinformatic analysis of the sequenced Pseudomonas plasmids allowed identification of their replication modules, REP (Figure 2, Tables S3 and S4). REP are basic genetic cassettes of plasmids that enable their autonomous replication in host cells, commonly used for classification of these replicons [59,60,61].…”

Section: Resultsmentioning

confidence: 99%

Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids

Romaniuk

Styczyński

Decewicz

et al. 2019

Genes

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Pseudomonas spp. are widely distributed in various environments around the world. They are also common in the Antarctic regions. To date, almost 200 plasmids of Pseudomonas spp. have been sequenced, but only 12 of them were isolated from psychrotolerant strains. In this study, 15 novel plasmids of cold-active Pseudomonas spp. originating from the King George Island (Antarctica) were characterized using a combined, structural and functional approach, including thorough genomic analyses, functional analyses of selected genetic modules, and identification of active transposable elements localized within the plasmids and comparative genomics. The analyses performed in this study increased the understanding of the horizontal transfer of plasmids found within Pseudomonas populations inhabiting Antarctic soils. It was shown that the majority of the studied plasmids are narrow-host-range replicons, whose transfer across taxonomic boundaries may be limited. Moreover, structural and functional analyses enabled identification and characterization of various accessory genetic modules, including genes encoding major pilin protein (PilA), that enhance biofilm formation, as well as active transposable elements. Furthermore, comparative genomic analyses revealed that the studied plasmids of Antarctic Pseudomonas spp. are unique, as they are highly dissimilar to the other known plasmids of Pseudomonas spp.

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Section: Resultsmentioning

confidence: 99%

Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids

Romaniuk

Styczyński

Decewicz

et al. 2019

Genes

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“…The shikimate pathway is the leading pathway for the synthesis of numerous aromatic compounds and phenazine antibiotics. The genetically engineered strains for the shikimate pathway have mainly been reported to be Escherichia coli [ 9 ], Bacillus subtilis [ 10 ], Corynebacterium glutamicum [ 11 ], Pseudomonas [ 7 , 12 ] and yeast [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Uncovering the Role of PhzC as DAHP Synthase in Shikimate Pathway of Pseudomonas chlororaphis HT66

Wang

Bilal

et al. 2022

Biology

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DAHP synthase catalyzes the first step in the shikimate pathway, deriving the biosynthesis of aromatic amino acids (Trp, Phe and Tyr), phenazine-1-carboxamide, folic acid, and ubiquinone in Pseudomonas chlororaphis. In this study, we identified and characterized one DAHP synthase encoding gene phzC, which differs from the reported DAHP synthase encoding genes aroF, aroG and aroH in E. coli. PhzC accounts for approximately 90% of the total DAHP synthase activities in P. chlororaphis HT66 and plays the most critical role in four DAHP synthases in the shikimate pathway. Inactivation of phzC resulted in the reduction of PCN production by more than 90%, while the absence of genes aroF, aroG and aroH reduced PCN yield by less than 15%, and the production of PCN was restored after the complementation of gene phzC. Moreover, the results showed that phzC in P. chlororaphis HT66 is not sensitive to feedback inhibition. This study demonstrated that gene phzC is essential for PCN biosynthesis. The expression level of both phzC and phzE genes are not inhibited in feedback by PCN production due to the absence of a loop region required for allosteric control reaction. This study highlighted the importance of PhzC and applying P. chlororaphis for shikimate pathway-derived high-value biological production.

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