Pseudomonas as Versatile Aromatics Cell Factory

Abstract

Aromatics and their derivatives are valuable chemicals with a plethora of important applications and thus play an integral role in modern society. Their current production relies mostly on the exploitation of petroleum resources. Independency from dwindling fossil resources and rising environmental concerns are major driving forces for the transition towards the production of sustainable aromatics from renewable feedstocks or waste streams. Whole‐cell biocatalysis is a promising strategy that allows the valorization of highly abundant, low‐cost substrates. In the last decades, extensive efforts are undertaken to allow the production of a wide spectrum of different aromatics and derivatives using microbes as biocatalysts. Pseudomonads are intriguing hosts for biocatalysis, as they display unique characteristics beneficial for the production of aromatics, including a distinct tolerance and versatile metabolism. This review highlights biotechnological applications of Pseudomonas as host for the production of aromatics and derived compounds. This includes their de novo biosynthesis from renewable resources, biotransformations in single‐ and biphasic fermentation setups, metabolic funneling of lignin‐derived aromatics, and the upcycling of aromatic monomers from plastic waste streams. Additionally, this review provides insights into unique features of Pseudomonads that make them exceptional hosts for aromatics biotechnology and discusses engineering strategies.

Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways

“…Bacteria have evolved numerous strategies to alleviate chemical stress and members of the Pseudomonas clade are especially well-equipped with such traits [1]. This has likely contributed to the development of the soil bacterium Pseudomonas putida and its relatives into versatile microbial cell factories during the past few decades, enabling the biosynthesis of various compounds including secondary metabolites like rhamnolipids, terpenes, polyketides, and non-ribosomal peptides, organic acids, alcohols, and aromatics [2][3][4]. Most of these products are not natively synthesised by the host strain, thus confronting cells with novel and -in parts -harmful chemistry, as many hydrophobic and antibiotic products Essays in Biochemistry (2021) EBC20200173 https://doi.org/10.1042/EBC20200173 New-to-Pseudomonas compound: A xenobiotic to a Pseudomonas host.…”

Microbial Degradation of Naphthalene and Substituted Naphthalenes: Metabolic Diversity and Genomic Insight for Bioremediation

“…Various physical and chemical cleanup methods like pyrolysis, oxidative thermal treatment, air-sparging, land-filling, incineration, etc., which are ineffective and expensive, have led to the generation of corrosive, toxic, and recalcitrant by-products. With the increase in global environmental awareness, microbes with the ability to degrade these pollutants and their derivatives (like halo-, nitro-, alkyl-, and/or methyl-) have received an increasing attention (Fennell et al, 2004;Haritash and Kaushik, 2009;Phale et al, 2020;Sarkar et al, 2020;Schwanemann et al, 2020). The use of these indigenous microbial candidates either alone or as mixed culture (consortia) for the removal of aromatic pollutants has been advantageous in terms of environmental safety, cost, efficiency, effectiveness, and sustainability.…”

Applied biocatalysis beyond just buffers – from aqueous to unconventional media. Options and guidelines

“…[73][74][75][76] This becomes particularly interesting when Pseudomonas is used as a host for the production of aromatic compounds from second-generation lignocellulosic raw materials or for the upcycling of aromatic monomers from plastic waste streams (Table 3). 77…”