Prunus spinosaFresh Fruit Juice: Antioxidant Activity in Cell-free and Cellular Systems

Abstract

The antioxidant activity was assessed of fresh juice from Prunus spinosa L. fruit (Rosaceae) growing wild in Urbino (central Italy) by using different cell-free in vitro analytical methods: 5-lipoxygenase test, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, and oxygen radical absorbance capacity (ORAC). Trolox was used as the reference antioxidant compound. In the 5-lipoxygenase and DPPH tests the fresh fruit juice of P. spinosa showed good antioxidant activity when compared with Trolox, while the ORAC value was 36.0 μmol eq. Trolox /g of fruit. These values are in accord with data reported in the literature for small fruits such as Vaccinium, Rubus and Ribes. The antioxidant capacity in cell-free systems of P. spinosa juice has been compared with its cytoprotective-bona fide antioxidant activity in cultured human promonocytes (U937 cells) exposed to hydrogen peroxide. The antioxidant activity of red berries has been correlated with their anthocyanin content. The results of this study indicate that the three most representative anthocyanins in P.spinosa fruit juice (cyanidin-3-rutinoside, peonidin-3-rutinoside and cyanidin-3-glucoside) are likely to play an important role in its antioxidant properties.

Evaluation of sensorial, phytochemical and biological properties of new isotonic beverages enriched with lemon and berries during shelf life

“…The açaí beverages (AA and LA) did not display any activity, being maqui samples the most active (3.07 ± 0.09 mmol L −1 Trolox for AM, and 2.86 ± 0.21 mmol L −1 Trolox for LM), also agreeing with previous reports of maqui extracts and maqui–lemon blends . A good antioxidant activity was also found in blackthorn juices and fruits …”

Wild Prunus Fruit Species as a Rich Source of Bioactive Compounds

“…Wild cherry fruits possessed lowest antioxidant activity (Table ). Various in vitro models showed that antioxidants form Prunus extract have potent cytoprotective effect, which should be promoted in phytochemical research (Fraternale and others ).…”

Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts

“…To the best of our knowledge, this is the first report of anthocyanins in blackthorn fruit acetone extract. Fraternale et al [46] previously identified cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside chloride and peonidin-3-O-rutinoside chloride in blackthorn fruits from Italy. A similar anthocyanin profile was determined by Veličković et al [11].…”