<i>Pr</i>-SNTX, a short-chain three-finger toxin from Papuan pigmy mulga snake, is an antagonist of muscle-type nicotinic acetylcholine receptor (α2βδε)

Yamauchi, Yoko; Kimoto, Hikari; Yang, Xiaoling; Filkin, Sergey Yu.; Utkin, Yuri N.; Kubo, Tai; Inagaki, Hidetoshi

doi:10.1080/09168451.2015.1065169

Cited by 3 publications

(7 citation statements)

References 13 publications

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“…SCNTX and Pr-SNTX are short-chain 3FTX that bind to muscle-type nAChR. There is no data regarding whether SCNTX binds to α7 nAChR, whereas Pr-SNTX has no antagonist activity on α7 nAChR at concentrations up to 10 μM [ 40 , 41 ]. Oh9-1 is an ω-bungarotoxin that lacks the positively charged amino acids conserved among α-neurotoxins, yet it binds the muscle-type nAChR with no binding to α7 nAChR up to 100 μM concentration [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

An Investigation of Three-Finger Toxin—nAChR Interactions through Rosetta Protein Docking

Gulsevin

Meiler

2020

Toxins

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Three-finger toxins (3FTX) are a group of peptides that affect multiple receptor types. One group of proteins affected by 3FTX are nicotinic acetylcholine receptors (nAChR). Structural information on how neurotoxins interact with nAChR is limited and is confined to a small group of neurotoxins. Therefore, in silico methods are valuable in understanding the interactions between 3FTX and different nAChR subtypes, but there are no established protocols to model 3FTX–nAChR interactions. We followed a homology modeling and protein docking protocol to address this issue and tested its success on three different systems. First, neurotoxin peptides co-crystallized with acetylcholine binding protein (AChBP) were re-docked to assess whether Rosetta protein–protein docking can reproduce the native poses. Second, experimental data on peptide binding to AChBP was used to test whether the docking protocol can qualitatively distinguish AChBP-binders from non-binders. Finally, we docked eight peptides with known α7 and muscle-type nAChR binding properties to test whether the protocol can explain the differential activities of the peptides at the two receptor subtypes. Overall, the docking protocol predicted the qualitative and some specific aspects of 3FTX binding to nAChR with reasonable success and shed light on unknown aspects of 3FTX binding to different receptor subtypes.

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Section: Resultsmentioning

confidence: 99%

An Investigation of Three-Finger Toxin—nAChR Interactions through Rosetta Protein Docking

Gulsevin

Meiler

2020

Toxins

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“…Pr-SNTX inhibited binding to muscle-type nAChR at nanomolar concentrations but showed no inhibitory activity in experiments with α7 nAChR up to a concentration of 10 μM [40].…”

Section: Pr-sntx May Bind To α7 Nachr With Weak Affinitymentioning

confidence: 91%

“…SCNTX and Pr-SNTX are short-chain 3FTX that bind to muscle-type nAChR. There is no data regarding whether SCNTX binds to α7 nAChR, whereas Pr-SNTX has no antagonist activity on α7 nAChR at concentrations up to 10 μM [39,40]. Oh9-1 is an ω-bungarotoxin that lacks the positively charged amino acids conserved among α-neurotoxins, yet it binds the muscle-type nAChR with no binding to α7 nAChR up to 100 μM concentration [41].…”

Section: Peptides With Diverse Properties Were Docked To α7 and Musclmentioning

confidence: 99%

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An Investigation of Three-finger Toxin – nAChR Interactions through Rosetta Protein Docking

Gulsevin¹,

Meiler²

2020

Preprint

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Three finger toxins (3FTX) are a group of peptides that affect multiple receptor types. One group of proteins affected by 3FTX are nicotinic acetylcholine receptors (nAChR). Structural information on how neurotoxins interact with nAChR is limited and are confined to a small group of neurotoxins. Therefore, in silico methods are valuable in understanding the interactions between 3FTX and different nAChR subtypes, but there are no established protocols to model 3FTX – nAChR interactions. We developed a homology modeling and protein docking protocol to address this issue and tested its success on three different systems. First, neurotoxin peptides co-crystallized with acetylcholine binding protein (AChBP) were re-docked to assess whether Rosetta protein – protein docking can reproduce the native poses. Second, experimental data on peptide binding to AChBP was used to test whether the docking protocol can qualitatively distinguish AChBP-binders from non-binders. Finally, we docked eight peptides with known α7 and muscle-type nAChR binding properties to test whether the protocol can explain the differential activities of the peptides at the two receptor subtypes. Overall, our docking protocol succeeded in predicting both qualitative and specific aspects of 3FTX binding to nAChR and shed light on some unknown aspects of 3FTX binding to different receptor subtypes.

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“…Depending on the mode of the aircraft, the cooling circuit can follow different paths. In this thermal management loop, the simulated parameters include Heat Exchanger, HEX [169], the mass fractions of different pipes (He, H 2 and Air) around the wall, and the thickness of the insulation layer. Figure 45.…”

Section: Flight Conditionmentioning

confidence: 99%

Thermal Protection System and Thermal Management for Combined-Cycle Engine: Review and Prospects

et al. 2019

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Combined-cycle engine is a potential propulsion system for hypersonic aircraft. To ensure long-term, normal operation of combined-cycle engine under the harsh environment of high thermal load, it is of great significance to study the thermal protection and management of the propulsion system. In this study, the objective and development status of thermal protection and thermal management systems for the combined-cycle propulsion system were described. The latest research progresses of thermal protection, thermal barrier coating, and thermal management system of the combined-cycle propulsion system were summarized. Moreover, the problems and shortcoming in current researches were summarized. In addition, a prospect for the future development of thermal protection and management of the combined-cycle propulsion system was presented, pointing out a direction of great value and vital research significance to thermal protection and management of the combined-cycle propulsion system.

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Pr-SNTX, a short-chain three-finger toxin from Papuan pigmy mulga snake, is an antagonist of muscle-type nicotinic acetylcholine receptor (α2βδε)

Cited by 3 publications

References 13 publications

An Investigation of Three-Finger Toxin—nAChR Interactions through Rosetta Protein Docking

An Investigation of Three-Finger Toxin—nAChR Interactions through Rosetta Protein Docking

An Investigation of Three-finger Toxin – nAChR Interactions through Rosetta Protein Docking

Thermal Protection System and Thermal Management for Combined-Cycle Engine: Review and Prospects

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