Portulaca oleracea L. Extract Ameliorates Intestinal Inflammation by Regulating Endoplasmic Reticulum Stress and Autophagy

Abstract

Scope To investigate the role of endoplasmic reticulum stress (ERS)‐induced autophagy in inflammatory bowel disease (IBD) and the intervention mechanism of Portulaca oleracea L. (POL) extract, a medicinal herb with anti‐inflammatory, antioxidant, immune‐regulating, and antitumor properties, in vitro and in vivo. Methods and Results An IL‐10‐deficient mouse model is used for in vivo experiments; a thapsigargin (Tg)‐stimulated ERS model of human colonic mucosal epithelial cells (HIECs) is used for in vitro experiments. The levels of ERS‐autophagy‐related proteins are examined by immunofluorescence and Western blot. Cellular ultrastructure is assessed with transmission electron microscopy. POL extract promotes a healing effect on colitis by regulating ERS‐autophagy through the protein kinase R‐like endoplasmic reticulum kinase (PERK)‐eukaryotic initiation factor 2α (eIF2α)/Beclin1‐microtubule‐associated protein light chain 3II (LC3II) pathway. Conclusion Overall, the results of this study further confirm the anti‐inflammatory mechanism and protective effect of POL extract and provide a new research avenue for the clinical treatment of IBD.

The Synergetic Effect of Egyptian Portulaca oleracea L. (Purslane) and Cichorium intybus L. (Chicory) Extracts against Glucocorticoid-Induced Testicular Toxicity in Rats through Attenuation of Oxidative Reactions and Autophagy

“…Previous studies are consistent with the current findings where the inhibition of ER stress can significantly inhibit autophagy’s overactivation and ultimately reduce testicular damage [ 23 , 104 , 107 ]. Recently, Purslane water extract alleviated inflammatory bowel disease by regulating ER stress and autophagy [ 142 ].…”

Identification of marker genes associated with N6-methyladenosine and autophagy in ulcerative colitis

“…Colitis was evaluated by disease activity index (DAI) score, including body weight, stool consistency, and fecal occult blood or gross bleeding[ 14 ]. DAI was calculated by grading on a scale of 0 to 4 using the following parameters: loss of body weight (0: normal; 1: 0%-5%; 2: 5%-10%; 3: 10%-15%; 4: > 15%); stool consistency (0: normal; 2: loose stools; 4: watery diarrhea); and occult blood (0: negative; 2: positive; 4: gross bleeding).…”

Phytochemical Characteristics and Anti‐Inflammatory, Immunoregulatory, and Antioxidant Effects of Portulaca oleracea L.: A Comprehensive Review

“…In a IL-10-deficient mouse model, treatment of piroxicam-accelerated colitis IL-10-deficient (PAC IL-10 −/− ) mice with 400 mg/kg/day P. oleracea extract and proinflammatory parameters such as TNF- α , IL1 β , and nuclear factor-kappa B (NF- κ B) were reduced which confirm anti-inflammatory effects of the extract [ 72 ]. Similarly, administration of a diet supplemented with 8% purslane plus 100 mg/l CdCl 2 in water for 8 weeks inhibited inflammation via suppressing some cytokines (TNF- α , IL-6, IL-1 β , and IFN- γ ) in the colon of mice [ 73 ].…”