<i>Porphyromonas gingivalis</i>: the gift of community involvement

Fletcher, Hansel M.

doi:10.1111/omi.12218

Cited by 4 publications

(2 citation statements)

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“…Periodontitis is a leading cause of tooth loss in adults as it destroys the connective tissue and alveolar bone (Pihlstrom et al., 2005). Porphyromonas gingivalis , a member of the “red complex,” is a gram‐negative etiologic agent for severe adult periodontitis (Fletcher, 2018). Recent studies have revealed a correlation between P. gingivalis and certain systemic diseases, including rheumatoid arthritis, Alzheimer's disease, and cardiovascular disease (Dominy et al., 2019; González‐Febles & Sanz, 2021; Klarström Engström et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

A GntR family transcription factor in Porphyromonas gingivalis regulates bacterial growth, acylpeptidyl oligopeptidase, and gingipains activity

Yang

Tan

Lei

et al. 2022

Molecular Oral Microbiology

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Porphyromonas gingivalis is a keystone pathogen for periodontitis. The function of the GntR family transcription factor is poorly studied in P. gingivalis. Numerous processes govern bacterial growth. The survival and pathogenicity of P. gingivalis depend heavily on its capacity to acquire amino acids as nutritional sources. In this investigation, a GntR transcription factor, pg1007, was identified in P. gingivalis, the deletion of which significantly inhibited bacterial growth. The mutant strain also exhibited an increased extracellular activity of gingipains and acylpeptidyl oligopeptidase (AOP). Global gene expression profiling revealed that the expression levels of 59 genes were significantly altered in the Δpg1007 mutant, with an upregulation in gene expression for AOP, ABC transporters, and some membrane proteins. In addition, His‐PG1007 protein was purified as a recombinant protein from Escherichia coli, and the conserved DNA sequence bound by it was determined using electrophoretic mobility shift assays and DNase I footprinting assays. Consequently, this study demonstrated that pg1007 is a crucial transcription factor in P. gingivalis and regulates the bacterial growth and activity of gingipains and AOP. These findings may enhance our understanding of the regulation of bacterial proliferation and protease activity in P. gingivalis.

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