Porocercospora seminalis gen. et comb. nov., the causal organism of buffalograss false smut

Amaradasa, Bimal S.; Madrid, Hugo; Groenewald, Johannes Z.; Crous, Pedro W.; Amundsen, Keenan

doi:10.3852/13-147

Cited by 22 publications

(12 citation statements)

References 28 publications

(26 reference statements)

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“…This genus is phylogenetically closely related to Bipolaris and Curvularia and is similar to them in having tretic conidiogenesis and darkly pigmented mycelium. However, Porocercospora has conidiophores without a geniculate rachis, conidiogenous cells with inconspicuous, non-darkened conidiogenous loci and long, obclavate to cylindro-obclavate conidia ( Amaradasa et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…DNA extraction of isolates of the other species studied was carried out from colonies growing on malt extract agar (Oxoid, Basingstoke, England) with the UltraClean® Microbial DNA Isolation Kit (Mo Bio Laboratories, Inc., Solana Beach, CA, USA). Amplification and sequencing of the ITS and RNA polymerase II second largest subunit (RPB2) were performed with primers ITS5 + ITS4 ( White et al 1990 ) and 5F2 + 7cR ( O’Donnell et al 2007 ) following the protocols of Amaradasa et al (2014) . Amplification of the gpd and LSU genes were performed with primers gpd1 + gpd2 ( Berbee et al 1999 ) and LR0R + LR5 ( Vilgalys & Hester 1990 ) as described in Manamgoda et al (2012) .…”

Section: Methodsmentioning

confidence: 99%

“… 1 BRIP: Queensland Plant Pathology Herbarium, Queensland, Australia; CBS: CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands; UTHSC: Fungus Testing Laboratory, University of Texas Health Science Center, San Antonio, Texas; T ex-type strain; NT ex-neotype strain ( Manamgoda et al 2012 ). 2 Sequences generated during this study appear in bold ; other sequences originate from Manamgoda et al (2012) , da Cunha et al (2013) and Amaradasa et al (2014) . …”

Section: Tablementioning

confidence: 99%

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Novel Curvularia species from clinical specimens

Madrid¹,

Cunha²,

Gené³

et al. 2014

Pers - Int Mycol J

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The fungal genus Curvularia includes numerous plant pathogens and some emerging opportunistic pathogens of humans. In a previous study we used morphology and sequences of the nuclear ribosomal internal transcribed spacer region (ITS) and the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene to identify species within a set of 99 clinical Curvularia isolates from the USA. Seventy-two isolates could be identified while the remaining 27 isolates belonged in three unclassified clades that were tentatively labelled Curvularia sp. I, II and III. In the present study, we further assess the taxonomic placement of these isolates using sequences of ITS, gpd, the large subunit rDNA, and the second largest subunit of RNA polymerase II. DNA sequence comparisons with a set of 87 isolates representing 33 Curvularia spp. and members of the closely-related genera Bipolaris and Exserohilum revealed that Curvularia sp. I, II and III represent novel lineages in Curvularia. These lineages are morphologically different from the currently accepted species. In the phylogenetic tree, Curvularia sp. I and sp. III were each split into two distinct lineages. Morphology and phylogeny supported the proposal of five new species, to be named C. americana, C. chlamydospora, C. hominis, C. muehlenbeckiae and C. pseudolunata. The concatenated 4-locus phylogeny revealed the existence of six clades in Curvularia, which are associated with particular morphological features. They were named after representative species, namely americana, eragrostidis, hominis, lunata, spicifera and trifolii.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Tablementioning

confidence: 99%

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Novel Curvularia species from clinical specimens

Madrid¹,

Cunha²,

Gené³

et al. 2014

Pers - Int Mycol J

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“…However, several newly segregated cercosporoid genera have been also introduced, or old genera resurrected to reflect monophyletic, morphologically separated clades, e.g. Pallidocercospora, Paracercospora (Crous et al 2013a), Phaeocercospora (Crous et al 2012), Neopseudocercospora (Crous et al 2013b) and Porocercospora (Amaradasa et al 2014). In this regard, many cercosporoid genera have been revealed to represent polyphyletic taxa.…”

Section: Introductionmentioning

confidence: 99%

Is morphology in Cercospora a reliable reflection of generic affinity?

et al. 2015

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Cercospora (Mycosphaerellaceae) is a large genus of fungi comprising many important plant pathogens. In recent years DNA-based studies have revealed multiple genera of cercosporoid fungi being poly-and paraphyletic. Among these genera, the genus Cercospora has always been perceived as monophyletic. In the present study, phylogenetic inferences based on partial gene sequences of the LSU, ITS, ACT, TEF1-α and HIS loci, elucidated a cercospora-like taxon from Ammi majus to cluster in a clade apart from Cercospora s. str. In spite of numerous Cercospora spp. presently known from their DNA sequence data, this collection represents the first concrete evidence to the fact that the morphological characters previously attributed to Cercospora s. str. evolved more than once in the Mycosphaerellaceae. The genus Neocercospora is subsequently introduced to accommodate the Iranian taxon occurring on A. majus. Further collections on other hosts and from different continents are now required to establish the prevalence and relative importance of species of Neocercospora.

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“…Species of Cercospora (Mycosphaerellaceae, Capnodiales) commonly occur associated with leaf and fruit spots on a range of cultivated and wild plants worldwide (Crous & Braun 2003, Amaradasa et al 2014, Bakhshi et al 2015b. To date there have been several studies focused on these fungi in Thailand, and more than 500 cercosporoid species have been identified (Giatgong 1980, Sontirat et al 1980, Petcharat & Kanjanamaneesathian 1989, Braun et al 2006, Meeboon et al 2007a, 2007b, 2007c, Nakashima et al 2007, Phengsintham et al 2013.…”

Section: Introductionmentioning

confidence: 99%

Taxonomy and phylogeny of Cercospora spp. from Northern Thailand

et al. 2015

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The genus Cercospora represents a group of important plant pathogenic fungi with a wide geographic distribution, being commonly associated with leaf spots on a broad range of plant hosts. The goal of the present study was to conduct a morphological and molecular phylogenetic analysis of the Cercospora spp. occurring on various plants growing in Northern Thailand, an area with a tropical savannah climate, and a rich diversity of vascular plants. Sixty Cercospora isolates were collected from 29 host species (representing 16 plant families). Partial nucleotide sequence data for two gene loci (ITS and cmdA), were generated for all isolates. Results from this study indicate that members of the genus Cercospora vary regarding host specificity, with some taxa having wide host ranges, and others being host-specific. Based on cultural, morphological and phylogenetic data, four new species of Cercospora could be identified: C. glycinicola (from Glycine max), C. cyperacearum and C. cyperina (from Cyperus alternifolius), and C. musigena (from Musa sp.). The most common Cercospora sp. found in Northern Thailand was C. cf. malloti, which occurred on a wide host range. Several collections could not be resolved to species level due to the lack of reference cultures and DNA data for morphologically similar species. Further collections from other countries are needed to help resolve the taxonomy of some species complexes occurring on various plant hosts in Thailand.

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Porocercospora seminalis gen. et comb. nov., the causal organism of buffalograss false smut

Cited by 22 publications

References 28 publications

Novel <I>Curvularia</I> species from clinical specimens

Novel <I>Curvularia</I> species from clinical specimens

Is morphology in Cercospora a reliable reflection of generic affinity?

Taxonomy and phylogeny of Cercospora spp. from Northern Thailand

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