<i>polyhomeotic</i>is required for somatic cell proliferation and differentiation during ovarian follicle formation in<i>Drosophila</i>

Narbonne, Karine; Besse, Florence; Brissard-Zahraoui, Jeanine; Pret, Anne-Marie; Busson, Denise

doi:10.1242/dev.01003

Cited by 23 publications

(23 citation statements)

References 64 publications

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“…Failure to adjust these two processes leads to 16-cell cyst packaging defects that manifest as compound egg chambers. These rare phenotypes had previously only been described upon perturbation of some signaling pathways, such as Notch, or Polycomb regulation (Jackson and Blochlinger, 1997;Narbonne et al, 2004).…”

Section: Discussionmentioning

confidence: 97%

Splice variants of the SWR1-type nucleosome remodeling factor Domino have distinct functions during Drosophila melanogaster oogenesis

Börner

Becker

2016

Development

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SWR1-type nucleosome remodeling factors replace histone H2A by variants to endow chromatin locally with specialized functionality. In Drosophila melanogaster a single H2A variant, H2A.V, combines functions of mammalian H2A.Z and H2A.X in transcription regulation and the DNA damage response. A major role in H2A.V incorporation for the only SWR1-like enzyme in flies, Domino, is assumed but not well documented in vivo. It is also unclear whether the two alternatively spliced isoforms, DOM-A and DOM-B, have redundant or specialized functions. Loss of both DOM isoforms compromises oogenesis, causing female sterility. We systematically explored roles of the two DOM isoforms during oogenesis using a cell type-specific knockdown approach. Despite their ubiquitous expression, DOM-A and DOM-B have non-redundant functions in germline and soma for egg formation. We show that chromatin incorporation of H2A.V in germline and somatic cells depends on DOM-B, whereas global incorporation in endoreplicating germline nurse cells appears to be independent of DOM. By contrast, DOM-A promotes the removal of H2A.V from stage 5 nurse cells. Remarkably, therefore, the two DOM isoforms have distinct functions in cell type-specific development and H2A.V exchange.

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Section: Discussionmentioning

confidence: 97%

Splice variants of the SWR1-type nucleosome remodeling factor Domino have distinct functions during Drosophila melanogaster oogenesis

Börner

Becker

2016

Development

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“…It was recently reported that the adult Drosophila midgut undergoes rapid cell turnover, similarly to the vertebrate intestine, and the fundamental similarities between Drosophila and vertebrate intestines have been revealed (Micchelli & Perrimon 2006;Ohlstein & Sparding 2006). The reproductive system is characterized by a high cellular proliferation rate (Narbonne et al 2004). Therefore, the need for DRE in dANT promoter activity in the gut as well as in reproductive systems may be associated with the cell-proliferation of these tissues.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional regulation of the Drosophila ANT gene by the DRE/DREF system

et al. 2007

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Adenine nucleotide translocase (ANT) is a crucial component in the maintenance of cellular energy homeostasis, as well as in the formation of the mitochondrial permeability transition pores. However, the molecular mechanisms regulating the expression of the ANT gene are poorly understood. In this study, we have identified three DNA replication-related elements (DRE; 5′ ′ ′ ′-TATCGATA) in the 5′ ′ ′ ′-flanking region of the Drosophila ANT (dANT ) gene. Gel-mobility shift analyses revealed that all three of the DREs were recognized by the DRE-binding factor (DREF). The site-directed mutagenesis of these DRE sites induces a considerable reduction in the activity of the dANT gene promoter in vitro. Analyses with transgenic flies harboring a dANT-lacZ fusion gene bearing the wild-type or mutant DRE sites showed that the DRE sites were required for the expression of dANT in vivo. We determined that the over-expression or knockdown of DREF exerts a regulatory effect on the activity of the dANT promoter. In addition, we observed the collapse of mitochondrial membrane potential in the eye imaginal discs in which DREF was overexpressed. These results show that DRE/DREF is a crucial regulator of dANT gene expression, and also suggest the possibility that cross-talk may occur between the DRE/DREF system and mitochondrial functioning.

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“…This underlines the importance of tissue specificity. Indeed, many studies have shown genetically that the PcG and TrxG genes have tissue-specific roles (Breen, 1999;Chanas and Maschat, 2005;Janody et al, 2004;Narbonne et al, 2004). It may well be that each type of adult stem cell in Drosophila uses a different set of PRE/TREs.…”

Section: Switching Upon Differentiation: Insights From Fliesmentioning

confidence: 99%

Polycomb/Trithorax response elements and epigenetic memory of cell identity

2007

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DEVELOPMENT 223Polycomb/Trithorax group response elements (PRE/TREs) are fascinating chromosomal pieces. Just a few hundred base pairs long, these elements can remember and maintain the active or silent transcriptional state of their associated genes for many cell generations, long after the initial determining activators and repressors have disappeared. Recently, substantial progress has been made towards understanding the nuts and bolts of PRE/TRE function at the molecular level and in experimentally mapping PRE/TRE sites across whole genomes. Here we examine the insights, controversies and new questions that have been generated by this recent flood of data. IntroductionDuring the 1990s, studies of the regulation of homeotic genes in the Drosophila Bithorax complex (BX-C) uncovered very different behaviour for two classes of cis-regulatory DNA element: initiator elements and maintenance elements (or Polycomb/Trithorax group response elements, PRE/TREs) (Busturia et al., 1989;Chan et al., 1994;Chiang et al., 1995;Simon et al., 1993;Simon et al., 1990) (reviewed by Maeda and Karch, 2006). One can think of these two types of elements as 'shift workers' that use very different strategies to regulate the expression patterns of the same genes at different stages of embryonic development. In the first three hours of development, the initiator elements are in control: the output of each homeotic gene depends on the local concentrations of segmentation gene products (these are activators and repressors that are present in different concentrations at different positions of the embryo). However, a few hours after these homeotic gene patterns have been established, the segmentation gene products decay, and thus the positional information they provide is lost. The transcriptional history of each gene is subsequently maintained throughout the rest of development, and into adulthood, by the ubiquitously expressed Polycomb group (PcG) and Trithorax group proteins (TrxG), which work antagonistically via the PRE/TRE elements to maintain active (TrxG) or silenced (PcG) transcriptional states (Moehrle and Paro, 1994). Although the effects of mutations in the PcG and TrxG genes are seen only after the segmentation gene products decay, the PcG and TrxG proteins themselves appear to associate with PRE/TREs much earlier, so that PRE/TREs are 'preloaded' with PcG and TrxG proteins, ready to maintain the transcriptional states that are set by the transiently acting segmentation gene products (Orlando et al., 1998).The maintenance of transcriptional memory at PRE/TREs is 'epigenetic'. This term has suffered much overuse and abuse in recent years, but we use here the classical definition given by Ptashne and Gann (Ptashne and Gann, 2002) (p100): "a change in the state of expression of a gene that does not involve a mutation, but that is nevertheless inherited (after cell division) in the absence of the signal (or event) that initiated that change". In the case of PRE/TREs, the information required to turn gene activity off or on after ea...

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polyhomeoticis required for somatic cell proliferation and differentiation during ovarian follicle formation inDrosophila

Cited by 23 publications

References 64 publications

Splice variants of the SWR1-type nucleosome remodeling factor Domino have distinct functions during Drosophila melanogaster oogenesis

Splice variants of the SWR1-type nucleosome remodeling factor Domino have distinct functions during Drosophila melanogaster oogenesis

Transcriptional regulation of the Drosophila ANT gene by the DRE/DREF system

Polycomb/Trithorax response elements and epigenetic memory of cell identity

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